Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study

The aim of this paper is the histopathological assessment of the effects of a newly synthesized nanomaterial based on calcium aluminate ALBO-CA on the pulp of rat teeth. In 18 Wistar rats, 54 Class I cavities and exposed pulp of maxillary molars were treated with the tested materials: two experiment...

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Main Authors: Janković Ognjenka, Paraš Smiljana, Adamović Tijana, Latinović Ljiljana Tadić, Arbutina Radmila, Đukić Igor, Marin Saša, Bulajić Marko, Vukoje Karolina, Jokanović Vukoman, Pavlić Verica
Format: Article
Language:English
Published: Sciendo 2025-06-01
Series:Acta Veterinaria
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Online Access:https://doi.org/10.2478/acve-2025-0017
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author Janković Ognjenka
Paraš Smiljana
Adamović Tijana
Latinović Ljiljana Tadić
Arbutina Radmila
Đukić Igor
Marin Saša
Bulajić Marko
Vukoje Karolina
Jokanović Vukoman
Pavlić Verica
author_facet Janković Ognjenka
Paraš Smiljana
Adamović Tijana
Latinović Ljiljana Tadić
Arbutina Radmila
Đukić Igor
Marin Saša
Bulajić Marko
Vukoje Karolina
Jokanović Vukoman
Pavlić Verica
author_sort Janković Ognjenka
collection DOAJ
description The aim of this paper is the histopathological assessment of the effects of a newly synthesized nanomaterial based on calcium aluminate ALBO-CA on the pulp of rat teeth. In 18 Wistar rats, 54 Class I cavities and exposed pulp of maxillary molars were treated with the tested materials: two experimental materials ALBO-CA (18 teeth), ALBO-CS (18 teeth), and MTA control (18 teeth), and cavities were restored with glass ionomer. The histopathological analysis included the following parameters: presence of pulp inflammation, degree of dentin bridge formation, and presence of bacteria in the pulp. Complete absence of pulp inflammation was noted in 12 (66.67 %) teeth with ALBO-CA, 10 (55.56 %) teeth with ALBO-CS, and 11 (60.95 %) teeth with MTA cement. A statistically significant difference in the results of pulp inflammatory response was found only when comparing the presence of a small number of polymorphonuclear leukocytes between ALBO-CS and MTA cement (Kruskal Wallis H test p=7.8255). A fully formed dentine bridge was recorded only after the application of ALBO-CA with a statistically significant difference compared to ALBO-CS and MTA (F test p=0.519, S-test p=0.656, Man-Whitney test p=2.802, Chi-square test p=4.747). Thirty days after the direct pulp capping with ALBO-CA, ALBO-CS, and MTA cements, bacteria were absent in rat teeth and surrounding tissue. Newly synthesized calcium aluminate ALBOCA showed good reparative abilities and possible use in direct pulp capping therapy.
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spelling doaj-art-1afa220d4b664e49a1216e31b45eb86c2025-08-20T02:37:13ZengSciendoActa Veterinaria1820-74482025-06-0175221223010.2478/acve-2025-0017Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo StudyJanković Ognjenka0Paraš Smiljana1Adamović Tijana2Latinović Ljiljana Tadić3Arbutina Radmila4Đukić Igor5Marin Saša6Bulajić Marko7Vukoje Karolina8Jokanović Vukoman9Pavlić Verica101University of Banja Luka, Faculty of Medicine, Department of Dental Diseases and Endodontics, the Republic of Srpska, Bosnia and Herzegovina2University of Banja Luka, Faculty of Science and Mathematics, Department of Cell Biology, Bosnia and Herzegovina3University of Banja Luka, Faculty of Medicine, Department of Periodontology and Oral Medicine, the Republic of Srpska, Bosnia and Herzegovina4Clinical Center, Institute of Pathology, Banja Luka, Bosnia and Herzegovina1University of Banja Luka, Faculty of Medicine, Department of Dental Diseases and Endodontics, the Republic of Srpska, Bosnia and Herzegovina5University of Banja Luka, Faculty of Medicine, Department of Oral Surgery, the Republic of Srpska, Bosnia and Herzegovina5University of Banja Luka, Faculty of Medicine, Department of Oral Surgery, the Republic of Srpska, Bosnia and Herzegovina5University of Banja Luka, Faculty of Medicine, Department of Oral Surgery, the Republic of Srpska, Bosnia and Herzegovina6University of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia7Institute of Nuclear Sciences “Vinča”, Belgrade, Serbia.3University of Banja Luka, Faculty of Medicine, Department of Periodontology and Oral Medicine, the Republic of Srpska, Bosnia and HerzegovinaThe aim of this paper is the histopathological assessment of the effects of a newly synthesized nanomaterial based on calcium aluminate ALBO-CA on the pulp of rat teeth. In 18 Wistar rats, 54 Class I cavities and exposed pulp of maxillary molars were treated with the tested materials: two experimental materials ALBO-CA (18 teeth), ALBO-CS (18 teeth), and MTA control (18 teeth), and cavities were restored with glass ionomer. The histopathological analysis included the following parameters: presence of pulp inflammation, degree of dentin bridge formation, and presence of bacteria in the pulp. Complete absence of pulp inflammation was noted in 12 (66.67 %) teeth with ALBO-CA, 10 (55.56 %) teeth with ALBO-CS, and 11 (60.95 %) teeth with MTA cement. A statistically significant difference in the results of pulp inflammatory response was found only when comparing the presence of a small number of polymorphonuclear leukocytes between ALBO-CS and MTA cement (Kruskal Wallis H test p=7.8255). A fully formed dentine bridge was recorded only after the application of ALBO-CA with a statistically significant difference compared to ALBO-CS and MTA (F test p=0.519, S-test p=0.656, Man-Whitney test p=2.802, Chi-square test p=4.747). Thirty days after the direct pulp capping with ALBO-CA, ALBO-CS, and MTA cements, bacteria were absent in rat teeth and surrounding tissue. Newly synthesized calcium aluminate ALBOCA showed good reparative abilities and possible use in direct pulp capping therapy.https://doi.org/10.2478/acve-2025-0017calcium aluminatescalcium silicatesdirect pulp cappingnanomaterials
spellingShingle Janković Ognjenka
Paraš Smiljana
Adamović Tijana
Latinović Ljiljana Tadić
Arbutina Radmila
Đukić Igor
Marin Saša
Bulajić Marko
Vukoje Karolina
Jokanović Vukoman
Pavlić Verica
Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
Acta Veterinaria
calcium aluminates
calcium silicates
direct pulp capping
nanomaterials
title Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
title_full Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
title_fullStr Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
title_full_unstemmed Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
title_short Application of Calcium Aluminate-Based Materials for Direct Pulp Capping – In Vivo Study
title_sort application of calcium aluminate based materials for direct pulp capping in vivo study
topic calcium aluminates
calcium silicates
direct pulp capping
nanomaterials
url https://doi.org/10.2478/acve-2025-0017
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