<strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>

A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion o...

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Main Authors: Zahra Hajializadeh, Mahdieh Asadi, Kamal Ahmadi, Asieh Balvasi
Format: Article
Language:English
Published: Acarological Society of Iran 2019-04-01
Series:Persian Journal of Acarology
Subjects:
Online Access:https://www.biotaxa.org/pja/article/view/43363
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author Zahra Hajializadeh
Mahdieh Asadi
Kamal Ahmadi
Asieh Balvasi
author_facet Zahra Hajializadeh
Mahdieh Asadi
Kamal Ahmadi
Asieh Balvasi
author_sort Zahra Hajializadeh
collection DOAJ
description A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.
format Article
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institution Kabale University
issn 2251-8169
language English
publishDate 2019-04-01
publisher Acarological Society of Iran
record_format Article
series Persian Journal of Acarology
spelling doaj-art-1af0de207d2d48e19d61c487ddac03532025-08-20T03:48:23ZengAcarological Society of IranPersian Journal of Acarology2251-81692019-04-018210.22073/pja.v8i2.43363<strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>Zahra Hajializadeh0Mahdieh Asadi1Kamal Ahmadi2Asieh Balvasi3Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, IranDepartment of plant protection, college of Agriculture, shahid Bahonar University of Kerman, IranDepartment of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Plant Protection, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.https://www.biotaxa.org/pja/article/view/43363Genetic diversityhaplotypeMesostigmatamitochondrial genomemolecular characterization.
spellingShingle Zahra Hajializadeh
Mahdieh Asadi
Kamal Ahmadi
Asieh Balvasi
<strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
Persian Journal of Acarology
Genetic diversity
haplotype
Mesostigmata
mitochondrial genome
molecular characterization.
title <strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
title_full <strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
title_fullStr <strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
title_full_unstemmed <strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
title_short <strong><em>Varroa destructor</em></strong><strong> (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI</strong>
title_sort strong em varroa destructor em strong strong acari varroidea populations from southern iran belong to haplotype k of the mitochondrial coi strong
topic Genetic diversity
haplotype
Mesostigmata
mitochondrial genome
molecular characterization.
url https://www.biotaxa.org/pja/article/view/43363
work_keys_str_mv AT zahrahajializadeh strongemvarroadestructoremstrongstrongacarivarroideapopulationsfromsoutherniranbelongtohaplotypekofthemitochondrialcoistrong
AT mahdiehasadi strongemvarroadestructoremstrongstrongacarivarroideapopulationsfromsoutherniranbelongtohaplotypekofthemitochondrialcoistrong
AT kamalahmadi strongemvarroadestructoremstrongstrongacarivarroideapopulationsfromsoutherniranbelongtohaplotypekofthemitochondrialcoistrong
AT asiehbalvasi strongemvarroadestructoremstrongstrongacarivarroideapopulationsfromsoutherniranbelongtohaplotypekofthemitochondrialcoistrong