Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells
The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R)-ginsenoside Rg3 (20(R)-Rg3) and 20(S)-ginsenoside Rg3 (20(S)-Rg3) were prepared by the method of chemical degradation and column chromatography, and the structu...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/848571 |
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| author | Gang Li Xiao-xiao Zhang Lin Lin Xiao-ning Liu Cheng-jun Ma Ji Li Chi-bo Wang |
| author_facet | Gang Li Xiao-xiao Zhang Lin Lin Xiao-ning Liu Cheng-jun Ma Ji Li Chi-bo Wang |
| author_sort | Gang Li |
| collection | DOAJ |
| description | The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R)-ginsenoside Rg3 (20(R)-Rg3) and 20(S)-ginsenoside Rg3 (20(S)-Rg3) were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy. MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h). We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h. The results indicated that both 20 (R)-Rg3 and 20 (S)-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells. Moreover, 20(R)-Rg3 exhibited better antioxidant activity than 20(S)-Rg3 in vitro. These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection. |
| format | Article |
| id | doaj-art-1af0827cfe4a487aae1a61d6f03fd822 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-1af0827cfe4a487aae1a61d6f03fd8222025-02-03T05:59:30ZengWileyJournal of Chemistry2090-90632090-90712014-01-01201410.1155/2014/848571848571Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH CellsGang Li0Xiao-xiao Zhang1Lin Lin2Xiao-ning Liu3Cheng-jun Ma4Ji Li5Chi-bo Wang6Life School of Yantai University, Yantai, Shandong 264005, ChinaLife School of Yantai University, Yantai, Shandong 264005, ChinaLife School of Yantai University, Yantai, Shandong 264005, ChinaLife School of Yantai University, Yantai, Shandong 264005, ChinaLife School of Yantai University, Yantai, Shandong 264005, ChinaLife School of Yantai University, Yantai, Shandong 264005, ChinaDepartment of Neurosurgery, Yantaishan Hospital, Yantai, Shandong 264000, ChinaThe aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R)-ginsenoside Rg3 (20(R)-Rg3) and 20(S)-ginsenoside Rg3 (20(S)-Rg3) were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy. MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h). We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h. The results indicated that both 20 (R)-Rg3 and 20 (S)-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells. Moreover, 20(R)-Rg3 exhibited better antioxidant activity than 20(S)-Rg3 in vitro. These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection.http://dx.doi.org/10.1155/2014/848571 |
| spellingShingle | Gang Li Xiao-xiao Zhang Lin Lin Xiao-ning Liu Cheng-jun Ma Ji Li Chi-bo Wang Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells Journal of Chemistry |
| title | Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells |
| title_full | Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells |
| title_fullStr | Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells |
| title_full_unstemmed | Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells |
| title_short | Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells |
| title_sort | preparation of ginsenoside rg3 and protection against h2o2 induced oxidative stress in human neuroblastoma sk n sh cells |
| url | http://dx.doi.org/10.1155/2014/848571 |
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