IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray
Objectives Research on the specific role of immunoglobulin G (IgG) glycosylation in SLE development and progression is limited, especially regarding changes in IgG glycosylation profiles among different SLE subtypes. In this study, we aimed to characterise the glycosylation profile of serum IgG in p...
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BMJ Publishing Group
2025-04-01
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| Series: | Lupus Science and Medicine |
| Online Access: | https://lupus.bmj.com/content/12/1/e001413.full |
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| author | Shangzhu Zhang Xiaofeng Zeng Qian Wang Jiuliang Zhao Xinping Tian Mengtao Li Yan Zhao Dong Xu Chaojun Hu Yang Wu Minhui Wang |
| author_facet | Shangzhu Zhang Xiaofeng Zeng Qian Wang Jiuliang Zhao Xinping Tian Mengtao Li Yan Zhao Dong Xu Chaojun Hu Yang Wu Minhui Wang |
| author_sort | Shangzhu Zhang |
| collection | DOAJ |
| description | Objectives Research on the specific role of immunoglobulin G (IgG) glycosylation in SLE development and progression is limited, especially regarding changes in IgG glycosylation profiles among different SLE subtypes. In this study, we aimed to characterise the glycosylation profile of serum IgG in patients with SLE.Methods Lectin microarrays with 56 lectins were used to analyse serum IgG glycosylation in 194 patients with SLE, 100 disease controls (40 primary Sjögren’s syndrome (pSS), 60 rheumatoid arthritis (RA)) and 100 healthy controls (HCs). Differences between SLE and control groups, as well as SLE subgroups, were validated by lectin blotting. Altered IgG glycosylation patterns were identified and further confirmed. Receiver operating characteristic (ROC) analysis evaluated the diagnostic value of these glycosylation changes in SLE and its subgroups, including neuropsychiatric SLE (NPSLE), lupus nephritis (LN), pulmonary arterial hypertension, immune thrombocytopaenia and SLE without major organ involvement (WMOI).Results Compared to DC and HC groups, the IgG glycan level of Galβ3GalNAc (binding Jacalin (11.3%) and Maclura pomifera lectin (14.4%)) was significantly increased, whereas most IgG glycan levels were significantly decreased, including core fucose, high mannose, GlcNAc, GalNAc and Galβ4GlcNAc in the SLE group (all p<0.05).The IgG glycan levels were elevated in GalNAc and galactose patterns in the NPSLE group compared to the WMOI group, as well as higher Galβ3GalNAc and galactose patterns in NPSLE and LN compared to HCs.Moreover, ROC curve analysis showed PNA levels might have moderate potential for discriminating SLE from pSS.Conclusions Patients with SLE show disease-specific alterations in serum IgG glycosylation, and aberrant Galβ3GalNAc, galactose and GalNAc glycosylation may have diagnostic value for SLE and NPSLE. Abnormal IgG glycans may provide new insights into their roles in SLE pathogenesis and progression. |
| format | Article |
| id | doaj-art-1af034f7839f4e38ad4da4e815c99c34 |
| institution | DOAJ |
| issn | 2053-8790 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Lupus Science and Medicine |
| spelling | doaj-art-1af034f7839f4e38ad4da4e815c99c342025-08-20T03:04:59ZengBMJ Publishing GroupLupus Science and Medicine2053-87902025-04-0112110.1136/lupus-2024-001413IgG glycosylation profiling of systemic lupus erythematosus using lectin microarrayShangzhu Zhang0Xiaofeng Zeng1Qian Wang2Jiuliang Zhao3Xinping Tian4Mengtao Li5Yan Zhao6Dong Xu7Chaojun Hu8Yang Wu9Minhui Wang10Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Beijing, China1 Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, State Key Laboratory of Complex Severe and Rare Diseases, The Ministry of Education Key Laboratory, Beijing, ChinaDepartment of Hepatobiliary Surgery, Northern Jiangsu People`s Hospital Affliated to Yangzhou University, Yangzhou City, Jiangsu Province, ChinaDepartment of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China1 Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, ChinaChinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaUT Southwestern Medical Center, Dallas, TX, USADepartment of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Beijing, ChinaChinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China1Institute of Molecular and Cell Biology (IMCB), A*-STAR, Singapore, SingaporeDepartment of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Beijing, ChinaObjectives Research on the specific role of immunoglobulin G (IgG) glycosylation in SLE development and progression is limited, especially regarding changes in IgG glycosylation profiles among different SLE subtypes. In this study, we aimed to characterise the glycosylation profile of serum IgG in patients with SLE.Methods Lectin microarrays with 56 lectins were used to analyse serum IgG glycosylation in 194 patients with SLE, 100 disease controls (40 primary Sjögren’s syndrome (pSS), 60 rheumatoid arthritis (RA)) and 100 healthy controls (HCs). Differences between SLE and control groups, as well as SLE subgroups, were validated by lectin blotting. Altered IgG glycosylation patterns were identified and further confirmed. Receiver operating characteristic (ROC) analysis evaluated the diagnostic value of these glycosylation changes in SLE and its subgroups, including neuropsychiatric SLE (NPSLE), lupus nephritis (LN), pulmonary arterial hypertension, immune thrombocytopaenia and SLE without major organ involvement (WMOI).Results Compared to DC and HC groups, the IgG glycan level of Galβ3GalNAc (binding Jacalin (11.3%) and Maclura pomifera lectin (14.4%)) was significantly increased, whereas most IgG glycan levels were significantly decreased, including core fucose, high mannose, GlcNAc, GalNAc and Galβ4GlcNAc in the SLE group (all p<0.05).The IgG glycan levels were elevated in GalNAc and galactose patterns in the NPSLE group compared to the WMOI group, as well as higher Galβ3GalNAc and galactose patterns in NPSLE and LN compared to HCs.Moreover, ROC curve analysis showed PNA levels might have moderate potential for discriminating SLE from pSS.Conclusions Patients with SLE show disease-specific alterations in serum IgG glycosylation, and aberrant Galβ3GalNAc, galactose and GalNAc glycosylation may have diagnostic value for SLE and NPSLE. Abnormal IgG glycans may provide new insights into their roles in SLE pathogenesis and progression.https://lupus.bmj.com/content/12/1/e001413.full |
| spellingShingle | Shangzhu Zhang Xiaofeng Zeng Qian Wang Jiuliang Zhao Xinping Tian Mengtao Li Yan Zhao Dong Xu Chaojun Hu Yang Wu Minhui Wang IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray Lupus Science and Medicine |
| title | IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| title_full | IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| title_fullStr | IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| title_full_unstemmed | IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| title_short | IgG glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| title_sort | igg glycosylation profiling of systemic lupus erythematosus using lectin microarray |
| url | https://lupus.bmj.com/content/12/1/e001413.full |
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