Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation
The present study was designed to investigate the impact of exercise training on lipogenic gene expression in liver and lipid partitioning following the ingestion of a high fructose load. Female rats were exercise-trained for 8 wk or kept sedentary before being submitted to a fasting/refeeding proto...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2012/181687 |
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| author | Siham Yasari Denis Prud'homme Frédérique Tesson Marek Jankowski Jolanta Gutkowska Emile Levy Jean-Marc Lavoie |
| author_facet | Siham Yasari Denis Prud'homme Frédérique Tesson Marek Jankowski Jolanta Gutkowska Emile Levy Jean-Marc Lavoie |
| author_sort | Siham Yasari |
| collection | DOAJ |
| description | The present study was designed to investigate the impact of exercise training on lipogenic gene expression in liver and lipid partitioning following the ingestion of a high fructose load. Female rats were exercise-trained for 8 wk or kept sedentary before being submitted to a fasting/refeeding protocol. Rats were further subdivided as follow: rats were fasted for 24 h, refed a standard diet for 24 h, starved for another 24 h, and refed with a standard or a high-fructose diet 24 h before sacrifice. Fructose refeeding was associated with an increase in hepatic lipid content, endocannabinoid receptor 1, sterol regulatory element-binding protein1c, and stearoyl-CoA desaturase1 gene expression in both Sed and TR rats. However, desaturation indexes measured in liver (C16 : 1/C16 : 0 and C18 : 1/C18 : 0) and plasma (C18 : 1/C18 : 0) were higher (P<0.01) in TR than in Sed rats following fructose refeeding. It is concluded that exercise training does not significantly affect fat accumulation and the molecular expression of genes involved in lipogenesis after fasting and fructose refeeding but does modify the partitioning of lipids so as to provide more unsaturated fatty acids in liver without affecting liver fat content. |
| format | Article |
| id | doaj-art-1ae58d5c100d452e80a1856b87931574 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-1ae58d5c100d452e80a1856b879315742025-02-03T05:51:41ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322012-01-01201210.1155/2012/181687181687Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat AccumulationSiham Yasari0Denis Prud'homme1Frédérique Tesson2Marek Jankowski3Jolanta Gutkowska4Emile Levy5Jean-Marc Lavoie6Department of Kinesiology, University of Montreal, C.P. 6128, Succursale Centre-Ville, Montréal , QC, H3C 3J7, CanadaBehavioural and Metabolic Research Unit, Montfort Hospital, University of Ottawa, Ottawa, ON, K1K 0T2, CanadaLaboratory of Genetics of Cardiac Diseases, University of Ottawa Heart Institute, Ottawa, ON, K1Y 4W7, CanadaResearch Center, Cardiovascular Biochemistry Laboratory, CHUM-Hôtel-Dieu, University of Montreal, Montreal, QC, H2W 1T8, CanadaResearch Center, Cardiovascular Biochemistry Laboratory, CHUM-Hôtel-Dieu, University of Montreal, Montreal, QC, H2W 1T8, CanadaResearch Center, Sainte-Justine Hospital, University of Montreal, Montreal, QC, H3T 1C5, CanadaDepartment of Kinesiology, University of Montreal, C.P. 6128, Succursale Centre-Ville, Montréal , QC, H3C 3J7, CanadaThe present study was designed to investigate the impact of exercise training on lipogenic gene expression in liver and lipid partitioning following the ingestion of a high fructose load. Female rats were exercise-trained for 8 wk or kept sedentary before being submitted to a fasting/refeeding protocol. Rats were further subdivided as follow: rats were fasted for 24 h, refed a standard diet for 24 h, starved for another 24 h, and refed with a standard or a high-fructose diet 24 h before sacrifice. Fructose refeeding was associated with an increase in hepatic lipid content, endocannabinoid receptor 1, sterol regulatory element-binding protein1c, and stearoyl-CoA desaturase1 gene expression in both Sed and TR rats. However, desaturation indexes measured in liver (C16 : 1/C16 : 0 and C18 : 1/C18 : 0) and plasma (C18 : 1/C18 : 0) were higher (P<0.01) in TR than in Sed rats following fructose refeeding. It is concluded that exercise training does not significantly affect fat accumulation and the molecular expression of genes involved in lipogenesis after fasting and fructose refeeding but does modify the partitioning of lipids so as to provide more unsaturated fatty acids in liver without affecting liver fat content.http://dx.doi.org/10.1155/2012/181687 |
| spellingShingle | Siham Yasari Denis Prud'homme Frédérique Tesson Marek Jankowski Jolanta Gutkowska Emile Levy Jean-Marc Lavoie Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation Journal of Nutrition and Metabolism |
| title | Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation |
| title_full | Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation |
| title_fullStr | Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation |
| title_full_unstemmed | Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation |
| title_short | Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation |
| title_sort | effects of exercise training on molecular markers of lipogenesis and lipid partitioning in fructose induced liver fat accumulation |
| url | http://dx.doi.org/10.1155/2012/181687 |
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