Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice
Abstract The healthy progression and termination of pregnancy depend critically upon embryo development, acquisition of uterine receptivity, implantation, and decidualization during the peri-implantation period. Sumoylation is a post-translational modification that affects the stability, transcripti...
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Nature Portfolio
2025-07-01
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| author | Ezgi Golal Cemre Nur Balci Nuray Acar |
| author_facet | Ezgi Golal Cemre Nur Balci Nuray Acar |
| author_sort | Ezgi Golal |
| collection | DOAJ |
| description | Abstract The healthy progression and termination of pregnancy depend critically upon embryo development, acquisition of uterine receptivity, implantation, and decidualization during the peri-implantation period. Sumoylation is a post-translational modification that affects the stability, transcriptional activity, and cellular localization of proteins. In our study, we investigated the localization and expression of SUMO1 (small ubiquitin-like modifier), SENP1 (sentrin-specific protease 1), and UBC9 (SUMO-conjugated enzyme UBC9), members of the sumoylation mechanism, in the mouse uterus and implantation sites during the peri-implantation period. Localization and expressions of SUMO1, SENP1, and UBC9 were determined by immunohistochemistry and Western blot, respectively. Estrogen (E2) and progesterone (P4) levels were measured by ELISA. SUMO1 was at the highest level in the luminal and gland epithelium and stroma on the 5th day of pregnancy compared to other pregnancy days. SENP1 was high on the 4th but low on the 5th day of pregnancy. On the 6th and 8th days of pregnancy, expressions of SUMO1 and SENP1 were decreased, while the amount of UBC9 was similar to other days of pregnancy. According to ELISA, E2 reached its highest level on the 4th day of pregnancy, and P4 reached its highest level on the 8th day of pregnancy. SUMO1, SENP1, and UBC9 are expressed at different levels in the uterus and implantation sites during early pregnancy. Our findings suggest that the sumoylation mechanism may play a role in the implantation and decidualization processes of mice. |
| format | Article |
| id | doaj-art-1ad9dfd2395145a1afe30994e9d3fb12 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-1ad9dfd2395145a1afe30994e9d3fb122025-08-20T04:01:36ZengNature PortfolioScientific Reports2045-23222025-07-0115111610.1038/s41598-025-07677-0Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in miceEzgi Golal0Cemre Nur Balci1Nuray Acar2Department of Histology and Embryology, Faculty of Medicine, Akdeniz UniversityDepartment of Histology and Embryology, Faculty of Medicine, Zonguldak Bulent Ecevit UniversityDepartment of Histology and Embryology, Faculty of Medicine, Akdeniz UniversityAbstract The healthy progression and termination of pregnancy depend critically upon embryo development, acquisition of uterine receptivity, implantation, and decidualization during the peri-implantation period. Sumoylation is a post-translational modification that affects the stability, transcriptional activity, and cellular localization of proteins. In our study, we investigated the localization and expression of SUMO1 (small ubiquitin-like modifier), SENP1 (sentrin-specific protease 1), and UBC9 (SUMO-conjugated enzyme UBC9), members of the sumoylation mechanism, in the mouse uterus and implantation sites during the peri-implantation period. Localization and expressions of SUMO1, SENP1, and UBC9 were determined by immunohistochemistry and Western blot, respectively. Estrogen (E2) and progesterone (P4) levels were measured by ELISA. SUMO1 was at the highest level in the luminal and gland epithelium and stroma on the 5th day of pregnancy compared to other pregnancy days. SENP1 was high on the 4th but low on the 5th day of pregnancy. On the 6th and 8th days of pregnancy, expressions of SUMO1 and SENP1 were decreased, while the amount of UBC9 was similar to other days of pregnancy. According to ELISA, E2 reached its highest level on the 4th day of pregnancy, and P4 reached its highest level on the 8th day of pregnancy. SUMO1, SENP1, and UBC9 are expressed at different levels in the uterus and implantation sites during early pregnancy. Our findings suggest that the sumoylation mechanism may play a role in the implantation and decidualization processes of mice.https://doi.org/10.1038/s41598-025-07677-0MouseSUMO1SENP1UBC9Uterus |
| spellingShingle | Ezgi Golal Cemre Nur Balci Nuray Acar Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice Scientific Reports Mouse SUMO1 SENP1 UBC9 Uterus |
| title | Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice |
| title_full | Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice |
| title_fullStr | Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice |
| title_full_unstemmed | Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice |
| title_short | Dynamic changes in sumoylation related proteins SUMO1, SENP1, and UBC9 during the peri implantation period in mice |
| title_sort | dynamic changes in sumoylation related proteins sumo1 senp1 and ubc9 during the peri implantation period in mice |
| topic | Mouse SUMO1 SENP1 UBC9 Uterus |
| url | https://doi.org/10.1038/s41598-025-07677-0 |
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