Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice

Rice often suffers from pests, diseases, and weeds throughout its life cycle. The management of pests, diseases, and weeds with agrochemicals increases production costs and results in pesticide residues. Developing new germplasm and varieties with multiple resistances is the most economical, sustain...

Full description

Saved in:
Bibliographic Details
Main Authors: Xinyan Li, Lüshui Weng, Youlun Xiao, Jinjiang Li, Lihua Deng, Qing Liu, Weiwei Kang, Yaping Duan, Daji Yang, Guoying Xiao
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Rice Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1672630825000447
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849233523980894208
author Xinyan Li
Lüshui Weng
Youlun Xiao
Jinjiang Li
Lihua Deng
Qing Liu
Weiwei Kang
Yaping Duan
Daji Yang
Guoying Xiao
author_facet Xinyan Li
Lüshui Weng
Youlun Xiao
Jinjiang Li
Lihua Deng
Qing Liu
Weiwei Kang
Yaping Duan
Daji Yang
Guoying Xiao
author_sort Xinyan Li
collection DOAJ
description Rice often suffers from pests, diseases, and weeds throughout its life cycle. The management of pests, diseases, and weeds with agrochemicals increases production costs and results in pesticide residues. Developing new germplasm and varieties with multiple resistances is the most economical, sustainable, and efficient way to solve these problems. In this study, marker-assisted backcrossing was used to pyramid multiple resistance genes, resulting in the development of a penta-resistance restorer line, B2A1920. This line was evaluated for its agronomic traits and resistance to diseases, pests, and herbicides. It was confirmed that B2A1920 contains the herbicide resistance gene Bar, the lepidopteran pest resistance gene Cry2Aa, the bacterial blight (BB) resistance gene Xa23, the blast resistance gene Pi2, and the brown planthopper (BPH) resistance genes Bph14 and Bph15. These genes confer resistance to glufosinate, lepidopteran pests, BB, blast, and BPH, respectively, while the line also exhibits elite agronomic traits. Its hybrids, Gui A × B2A1920, Quan 9311A × B2A1920, Yexiang A × B2A1920, Xianglong A × B2A1920, Tiantai A × B2A1920, and Shen 9A × B2A1920, likewise showed penta-resistance and superior agronomic performance. These results indicate the successful development of a cytoplasmic male sterile restorer line with high combining ability and penta-resistance.
format Article
id doaj-art-1abe7b04a06d4146bb3ed4606acd6a1d
institution Kabale University
issn 1672-6308
language English
publishDate 2025-07-01
publisher Elsevier
record_format Article
series Rice Science
spelling doaj-art-1abe7b04a06d4146bb3ed4606acd6a1d2025-08-20T05:06:04ZengElsevierRice Science1672-63082025-07-0132451252410.1016/j.rsci.2025.02.003Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid RiceXinyan Li0Lüshui Weng1Youlun Xiao2Jinjiang Li3Lihua Deng4Qing Liu5Weiwei Kang6Yaping Duan7Daji Yang8Guoying Xiao9Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaHunan Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China; Corresponding author.Rice often suffers from pests, diseases, and weeds throughout its life cycle. The management of pests, diseases, and weeds with agrochemicals increases production costs and results in pesticide residues. Developing new germplasm and varieties with multiple resistances is the most economical, sustainable, and efficient way to solve these problems. In this study, marker-assisted backcrossing was used to pyramid multiple resistance genes, resulting in the development of a penta-resistance restorer line, B2A1920. This line was evaluated for its agronomic traits and resistance to diseases, pests, and herbicides. It was confirmed that B2A1920 contains the herbicide resistance gene Bar, the lepidopteran pest resistance gene Cry2Aa, the bacterial blight (BB) resistance gene Xa23, the blast resistance gene Pi2, and the brown planthopper (BPH) resistance genes Bph14 and Bph15. These genes confer resistance to glufosinate, lepidopteran pests, BB, blast, and BPH, respectively, while the line also exhibits elite agronomic traits. Its hybrids, Gui A × B2A1920, Quan 9311A × B2A1920, Yexiang A × B2A1920, Xianglong A × B2A1920, Tiantai A × B2A1920, and Shen 9A × B2A1920, likewise showed penta-resistance and superior agronomic performance. These results indicate the successful development of a cytoplasmic male sterile restorer line with high combining ability and penta-resistance.http://www.sciencedirect.com/science/article/pii/S1672630825000447hybrid riceresistancenon-selective herbiciderice blastbacterial blightlepidopteran pest
spellingShingle Xinyan Li
Lüshui Weng
Youlun Xiao
Jinjiang Li
Lihua Deng
Qing Liu
Weiwei Kang
Yaping Duan
Daji Yang
Guoying Xiao
Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
Rice Science
hybrid rice
resistance
non-selective herbicide
rice blast
bacterial blight
lepidopteran pest
title Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
title_full Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
title_fullStr Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
title_full_unstemmed Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
title_short Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice
title_sort characteristic analysis of penta resistance restorer line for hybrid rice
topic hybrid rice
resistance
non-selective herbicide
rice blast
bacterial blight
lepidopteran pest
url http://www.sciencedirect.com/science/article/pii/S1672630825000447
work_keys_str_mv AT xinyanli characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT lushuiweng characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT youlunxiao characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT jinjiangli characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT lihuadeng characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT qingliu characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT weiweikang characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT yapingduan characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT dajiyang characteristicanalysisofpentaresistancerestorerlineforhybridrice
AT guoyingxiao characteristicanalysisofpentaresistancerestorerlineforhybridrice