An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells
Soluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut. We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation of T cells. Peripheral bl...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/879843 |
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| author | Ashok Kumar Kumawat Nils Nyhlin Anna Wickbom Curt Tysk Johan Bohr Olof Hultgren Elisabeth Hultgren Hörnquist |
| author_facet | Ashok Kumar Kumawat Nils Nyhlin Anna Wickbom Curt Tysk Johan Bohr Olof Hultgren Elisabeth Hultgren Hörnquist |
| author_sort | Ashok Kumar Kumawat |
| collection | DOAJ |
| description | Soluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut. We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation of T cells. Peripheral blood CD4+ T cells from healthy donors were polyclonally activated in the presence of conditioned medium (CM) generated from denuded biopsies (DNB) or isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients compared to noninflamed controls, to determine proliferation and secretion of cytokines involved in T-cell differentiation. Compared to controls, we observed significantly increased production of the proinflammatory cytokines IFN-γ, IL-17A, IL-6, and IL-1β and the anti-inflammatory cytokines IL-4 and IL-10 in the presence of CC-DNB-CM. The most pronounced effect of CC-LPMC-CM on peripheral CD4+ T cells was a trend towards increased production of IL-17A and IL-10. A trend towards reduced inhibition of T-cell proliferation was noted in the presence of CC-DNB-CM. In conclusion, our in vitro model reveals implications of soluble factors from CC colonic mucosa on peripheral T cells, enhancing their production of both pro- and anti-inflammatory cytokines. |
| format | Article |
| id | doaj-art-1aba2df565f344faae12bed5a9a5de51 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1aba2df565f344faae12bed5a9a5de512025-08-20T03:54:24ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/879843879843An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T CellsAshok Kumar Kumawat0Nils Nyhlin1Anna Wickbom2Curt Tysk3Johan Bohr4Olof Hultgren5Elisabeth Hultgren Hörnquist6School of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenSchool of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenSchool of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenSchool of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenSchool of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenDepartment of Microbiology and Immunology, Örebro University Hospital, 70185 Örebro, SwedenSchool of Health and Medical Sciences, Örebro University, 70182 Örebro, SwedenSoluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut. We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation of T cells. Peripheral blood CD4+ T cells from healthy donors were polyclonally activated in the presence of conditioned medium (CM) generated from denuded biopsies (DNB) or isolated lamina propria mononuclear cells (LPMCs) from mucosal biopsies from CC patients compared to noninflamed controls, to determine proliferation and secretion of cytokines involved in T-cell differentiation. Compared to controls, we observed significantly increased production of the proinflammatory cytokines IFN-γ, IL-17A, IL-6, and IL-1β and the anti-inflammatory cytokines IL-4 and IL-10 in the presence of CC-DNB-CM. The most pronounced effect of CC-LPMC-CM on peripheral CD4+ T cells was a trend towards increased production of IL-17A and IL-10. A trend towards reduced inhibition of T-cell proliferation was noted in the presence of CC-DNB-CM. In conclusion, our in vitro model reveals implications of soluble factors from CC colonic mucosa on peripheral T cells, enhancing their production of both pro- and anti-inflammatory cytokines.http://dx.doi.org/10.1155/2014/879843 |
| spellingShingle | Ashok Kumar Kumawat Nils Nyhlin Anna Wickbom Curt Tysk Johan Bohr Olof Hultgren Elisabeth Hultgren Hörnquist An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells Mediators of Inflammation |
| title | An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells |
| title_full | An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells |
| title_fullStr | An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells |
| title_full_unstemmed | An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells |
| title_short | An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4+ T Cells |
| title_sort | in vitro model to evaluate the impact of the soluble factors from the colonic mucosa of collagenous colitis patients on t cells enhanced production of il 17a and il 10 from peripheral cd4 t cells |
| url | http://dx.doi.org/10.1155/2014/879843 |
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