Polysaccharides from Marine Bacteria and Their Anti-SARS-CoV-2 Activity
This study investigated the anti-SARS-CoV-2 activity of Polysaccharides (PSs) from three species of marine bacteria (<i>Alteromonas nigrifaciens</i> KMM 156, <i>Cobetia amphilecti</i> KMM 3890, and <i>Idiomarina abyssalis</i> KMM 227<sup>T</sup>). The...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/16/5/102 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study investigated the anti-SARS-CoV-2 activity of Polysaccharides (PSs) from three species of marine bacteria (<i>Alteromonas nigrifaciens</i> KMM 156, <i>Cobetia amphilecti</i> KMM 3890, and <i>Idiomarina abyssalis</i> KMM 227<sup>T</sup>). The chemical structure of PSs from marine bacteria is characterized using <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including 2D NMR experiments. PS from <i>A. nigrifaciens</i> KMM 156 consists of tetrasaccharide repeating units containing two L-rhamnose residues and one residue each of 2-acetamido-2-deoxy-D-glucose and an ether of D-glucose with (<i>R</i>)-lactic acid, 3-<i>O</i>-[(<i>R</i>)-1-carboxyethyl]-D-glucose. PS from <i>C. amphilecti</i> KMM 3890 is constructed from branched trisaccharide repeating units consisting of D-glucose, D-mannose, and sulfated 3-deoxy-D-<i>manno</i>-oct-2-ulosonic acid. A unique PS from deep-sea marine bacterium <i>I. abyssalis</i> KMM 227<sup>T</sup> consists of branched pentasaccharide repeating units and is characterized by the presence of a rare bacterial polysaccharide component 2-<i>O</i>-sulfate-3-<i>N</i>-(4-hydroxybutanoyl)-3,6-dideoxy-D-glucose. The activity of PSs against SARS-CoV-2 was assessed by inhibition of the virus cytopathogenic effect (<i>CI</i>) in the methylthiazolyl tetrazolium (MTT) test and using a real-time reverse transcription polymerase chain reaction (RT-PCR-RV). Results of the study demonstrate that PSs, which differ in chemical structure, exhibited anti-SARS-CoV-2 activity differences. This is confirmed both in the test of inhibition of the virus <i>CI</i> and in the reduction in the SARS-CoV-2 virus RNA level. PSs from <i>A. nigrifaciens</i> KMM 156 exhibited the strongest anti-SARS-CoV-2 effect, effectively inhibiting the stages of attachment and penetration of SARS-CoV-2 into the cells. |
|---|---|
| ISSN: | 2036-7481 |