Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging
Abstract Chemiluminescence (CL) is a self-illuminating phenomenon fueled by chemical energy instead of extra excited light, which features superiority in sensitivity, signal-to-background ratios, and imaging depth. Strategies to synthesize a CL emission unimolecular skeleton in the second near-infra...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55503-4 |
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| author | Zhongxiang Chen Qian Li Ying Wu Jianyong Liu Luntao Liu Lichao Su Rongrong Wu Jibin Song |
| author_facet | Zhongxiang Chen Qian Li Ying Wu Jianyong Liu Luntao Liu Lichao Su Rongrong Wu Jibin Song |
| author_sort | Zhongxiang Chen |
| collection | DOAJ |
| description | Abstract Chemiluminescence (CL) is a self-illuminating phenomenon fueled by chemical energy instead of extra excited light, which features superiority in sensitivity, signal-to-background ratios, and imaging depth. Strategies to synthesize a CL emission unimolecular skeleton in the second near-infrared window (NIR-II) and a unimolecular probe with direct duplex NIR-II [CL/fluorescence (FL)] emission are lacking. Here, we employ modular synthesis routes to construct a series of directly activated NIR-II CL emission unimolecular probes with a maximum emission wavelength of up to 1060 nm, and use them for real-time and continuous detection of the superoxide anion generated in acetaminophen induced liver injury in a female mice model under both NIR-II CL and NIR-II FL imaging channels. Thus, this study establishes a directly activatable NIR-II CL emission unimolecular skeleton, validating the scalability of this duplex NIR-II CL/FL imaging platform in bioactive molecule detection and disease diagnosis. |
| format | Article |
| id | doaj-art-1ab39356311a42309bfd540f11e26077 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-1ab39356311a42309bfd540f11e260772025-01-05T12:36:54ZengNature PortfolioNature Communications2041-17232025-01-0116111210.1038/s41467-024-55503-4Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex ImagingZhongxiang Chen0Qian Li1Ying Wu2Jianyong Liu3Luntao Liu4Lichao Su5Rongrong Wu6Jibin Song7State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical TechnologyMOE Key Laboratory for Analytical Science of Food Safety and Biology Institution, College of Chemistry, Fuzhou UniversityState Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical TechnologyMOE Key Laboratory for Analytical Science of Food Safety and Biology Institution, College of Chemistry, Fuzhou UniversityMOE Key Laboratory for Analytical Science of Food Safety and Biology Institution, College of Chemistry, Fuzhou UniversityMOE Key Laboratory for Analytical Science of Food Safety and Biology Institution, College of Chemistry, Fuzhou UniversityMOE Key Laboratory for Analytical Science of Food Safety and Biology Institution, College of Chemistry, Fuzhou UniversityState Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical TechnologyAbstract Chemiluminescence (CL) is a self-illuminating phenomenon fueled by chemical energy instead of extra excited light, which features superiority in sensitivity, signal-to-background ratios, and imaging depth. Strategies to synthesize a CL emission unimolecular skeleton in the second near-infrared window (NIR-II) and a unimolecular probe with direct duplex NIR-II [CL/fluorescence (FL)] emission are lacking. Here, we employ modular synthesis routes to construct a series of directly activated NIR-II CL emission unimolecular probes with a maximum emission wavelength of up to 1060 nm, and use them for real-time and continuous detection of the superoxide anion generated in acetaminophen induced liver injury in a female mice model under both NIR-II CL and NIR-II FL imaging channels. Thus, this study establishes a directly activatable NIR-II CL emission unimolecular skeleton, validating the scalability of this duplex NIR-II CL/FL imaging platform in bioactive molecule detection and disease diagnosis.https://doi.org/10.1038/s41467-024-55503-4 |
| spellingShingle | Zhongxiang Chen Qian Li Ying Wu Jianyong Liu Luntao Liu Lichao Su Rongrong Wu Jibin Song Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging Nature Communications |
| title | Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging |
| title_full | Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging |
| title_fullStr | Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging |
| title_full_unstemmed | Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging |
| title_short | Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging |
| title_sort | molecular engineering of direct activated nir ii chemiluminescence platform for in vivo chemiluminescence fluorescence duplex imaging |
| url | https://doi.org/10.1038/s41467-024-55503-4 |
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