Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro

Miracle fruit (Synsepalum dulcificum) has been well known and studied for its unique taste-modifying ability. In this study, the monosaccharide composition, molecular weight (Mw), and in vitro bioactivities (antioxidant, α-glucosidase inhibition) of polysaccharides from the seeds (MFP-S) and leaves...

Full description

Saved in:
Bibliographic Details
Main Authors: Huajun Jian, Fang Qiao, Jie Chen, Nongyue He
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2017/8948639
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832552897615757312
author Huajun Jian
Fang Qiao
Jie Chen
Nongyue He
author_facet Huajun Jian
Fang Qiao
Jie Chen
Nongyue He
author_sort Huajun Jian
collection DOAJ
description Miracle fruit (Synsepalum dulcificum) has been well known and studied for its unique taste-modifying ability. In this study, the monosaccharide composition, molecular weight (Mw), and in vitro bioactivities (antioxidant, α-glucosidase inhibition) of polysaccharides from the seeds (MFP-S) and leaves (MFP-L) of miracle fruit were investigated. The results showed that MFP-S was a homogeneous polysaccharide (Mw 2804 Da) with glucose. MFP-L displayed three fractions (92093, 1496, and 237 Da) consisting of rhamnose, arabinose, galactose, glucose, and xylose. Moreover, the antioxidant and α-glucosidase inhibition of MFP-L were significantly greater than those of MFP-S. The α-glucosidase inhibition of MFP-L was remarkably better than the positive control, acarbose (an antidiabetes drug). More specifically, the 50% inhibitory concentration (IC50) values of α-glucosidase activities for MFP-S, MFP-L, and acarbose were 33, 0.01, and 1 mg mL−1, separately. Therefore, MFP-L can be developed as a functional factor with both antioxidant and antidiabetes activities in food applications.
format Article
id doaj-art-1a947a28d6f140d78d19ea81977488f7
institution Kabale University
issn 2090-9063
2090-9071
language English
publishDate 2017-01-01
publisher Wiley
record_format Article
series Journal of Chemistry
spelling doaj-art-1a947a28d6f140d78d19ea81977488f72025-02-03T05:57:27ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/89486398948639Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In VitroHuajun Jian0Fang Qiao1Jie Chen2Nongyue He3School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, ChinaSchool of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu 210096, ChinaMiracle fruit (Synsepalum dulcificum) has been well known and studied for its unique taste-modifying ability. In this study, the monosaccharide composition, molecular weight (Mw), and in vitro bioactivities (antioxidant, α-glucosidase inhibition) of polysaccharides from the seeds (MFP-S) and leaves (MFP-L) of miracle fruit were investigated. The results showed that MFP-S was a homogeneous polysaccharide (Mw 2804 Da) with glucose. MFP-L displayed three fractions (92093, 1496, and 237 Da) consisting of rhamnose, arabinose, galactose, glucose, and xylose. Moreover, the antioxidant and α-glucosidase inhibition of MFP-L were significantly greater than those of MFP-S. The α-glucosidase inhibition of MFP-L was remarkably better than the positive control, acarbose (an antidiabetes drug). More specifically, the 50% inhibitory concentration (IC50) values of α-glucosidase activities for MFP-S, MFP-L, and acarbose were 33, 0.01, and 1 mg mL−1, separately. Therefore, MFP-L can be developed as a functional factor with both antioxidant and antidiabetes activities in food applications.http://dx.doi.org/10.1155/2017/8948639
spellingShingle Huajun Jian
Fang Qiao
Jie Chen
Nongyue He
Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
Journal of Chemistry
title Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
title_full Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
title_fullStr Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
title_full_unstemmed Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
title_short Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro
title_sort physicochemical characterisation of polysaccharides from the seeds and leaves of miracle fruit synsepalum dulcificum and their antioxidant and α glucosidase inhibitory activities in vitro
url http://dx.doi.org/10.1155/2017/8948639
work_keys_str_mv AT huajunjian physicochemicalcharacterisationofpolysaccharidesfromtheseedsandleavesofmiraclefruitsynsepalumdulcificumandtheirantioxidantandaglucosidaseinhibitoryactivitiesinvitro
AT fangqiao physicochemicalcharacterisationofpolysaccharidesfromtheseedsandleavesofmiraclefruitsynsepalumdulcificumandtheirantioxidantandaglucosidaseinhibitoryactivitiesinvitro
AT jiechen physicochemicalcharacterisationofpolysaccharidesfromtheseedsandleavesofmiraclefruitsynsepalumdulcificumandtheirantioxidantandaglucosidaseinhibitoryactivitiesinvitro
AT nongyuehe physicochemicalcharacterisationofpolysaccharidesfromtheseedsandleavesofmiraclefruitsynsepalumdulcificumandtheirantioxidantandaglucosidaseinhibitoryactivitiesinvitro