Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis
Purpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. Methods. Seventy-five female Lewis rats were...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2007/93857 |
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| author | Victor E. Reviglio Ruben H. Sambuelli Alejandra Olmedo Micaela Falco Jose Echenique Terrence P. O'Brien Irene C. Kuo |
| author_facet | Victor E. Reviglio Ruben H. Sambuelli Alejandra Olmedo Micaela Falco Jose Echenique Terrence P. O'Brien Irene C. Kuo |
| author_sort | Victor E. Reviglio |
| collection | DOAJ |
| description | Purpose. To describe the presence of secretory leukocyte protease
inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune
reaction in a rat model of
Staphylococcus aureus endophthalmitis.
Methods. Seventy-five female Lewis rats were divided into three groups: the endophthalmitis
group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the
vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not
injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and
Western blot studies for SLPI.
Results. In S. aureus endophthalmitis eyes, there was strong
immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas
also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By
immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was
identified in all samples from the endophthalmitis group by traditional culture methods.
Conclusions. To our knowledge, this report is the first to demonstrate the presence of SLPI
in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus
endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by
inflammatory cells in the anterior and posterior segments may contribute to the host defense response against
infectious endophthalmitis. |
| format | Article |
| id | doaj-art-1a7dc893613240df9bfadd02860fdbfd |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1a7dc893613240df9bfadd02860fdbfd2025-02-03T01:29:55ZengWileyMediators of Inflammation0962-93511466-18612007-01-01200710.1155/2007/9385793857Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus EndophthalmitisVictor E. Reviglio0Ruben H. Sambuelli1Alejandra Olmedo2Micaela Falco3Jose Echenique4Terrence P. O'Brien5Irene C. Kuo6Cornea and External Eye Diseases Lab, School of Medicine, Catholic University of Cordoba, Cordoba 5021, ArgentinaCornea and External Eye Diseases Lab, School of Medicine, Catholic University of Cordoba, Cordoba 5021, ArgentinaCornea and External Eye Diseases Lab, School of Medicine, Catholic University of Cordoba, Cordoba 5021, ArgentinaCornea and External Eye Diseases Lab, School of Medicine, Catholic University of Cordoba, Cordoba 5021, ArgentinaDepartment of Biochemistry and Molecular Biology, School of Medicine, National University of Cordoba, Cordoba 5022, ArgentinaCornea and External Diseases, Bascom Palmer, School of Medicine, University of Miami, Miami, FL 33136, USACornea Department, The Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD 21287, USAPurpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. Methods. Seventy-five female Lewis rats were divided into three groups: the endophthalmitis group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and Western blot studies for SLPI. Results. In S. aureus endophthalmitis eyes, there was strong immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was identified in all samples from the endophthalmitis group by traditional culture methods. Conclusions. To our knowledge, this report is the first to demonstrate the presence of SLPI in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by inflammatory cells in the anterior and posterior segments may contribute to the host defense response against infectious endophthalmitis.http://dx.doi.org/10.1155/2007/93857 |
| spellingShingle | Victor E. Reviglio Ruben H. Sambuelli Alejandra Olmedo Micaela Falco Jose Echenique Terrence P. O'Brien Irene C. Kuo Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis Mediators of Inflammation |
| title | Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis |
| title_full | Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis |
| title_fullStr | Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis |
| title_full_unstemmed | Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis |
| title_short | Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis |
| title_sort | secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in staphylococcus aureus endophthalmitis |
| url | http://dx.doi.org/10.1155/2007/93857 |
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