Physically modification treatments improved high internal phase emulsions stabilized by walnut protein

Walnut protein (WP)-based emulsions are notable for their sustainability and biocompatibility. However, their emulsifying capability is often hindered by a densely packed structure. This study investigates the impact of physical modifications, specifically high-speed shearing, heat treatment, and ul...

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Main Authors: Min Yang, Yunkun Zhu, Minwei Zhang, Youning Zhang, Zhongkai Zhao, Liang Wang, Jie Yang, Jun Liu, Yanan Qin
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525003554
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author Min Yang
Yunkun Zhu
Minwei Zhang
Youning Zhang
Zhongkai Zhao
Liang Wang
Jie Yang
Jun Liu
Yanan Qin
author_facet Min Yang
Yunkun Zhu
Minwei Zhang
Youning Zhang
Zhongkai Zhao
Liang Wang
Jie Yang
Jun Liu
Yanan Qin
author_sort Min Yang
collection DOAJ
description Walnut protein (WP)-based emulsions are notable for their sustainability and biocompatibility. However, their emulsifying capability is often hindered by a densely packed structure. This study investigates the impact of physical modifications, specifically high-speed shearing, heat treatment, and ultrasonic treatment, on the structure of WP and examines the subsequent effects on the physicochemical properties and stability of the resulting high internal phase emulsions (HIPEs). The three modification methods reduced the average WP particle diameter by 55.38 %, 48.31 %, and 73.66 %, respectively, the corresponding D4,3 values of HIPEs decreased by 41.31 %, 36.77 %, and 50.43 %. Additionally, the gel-like properties and stability of the HIPEs from modified WP significantly improved. Notably, ultrasonic treatment exhibited the most substantial influence, as cavitation effectively unfolded the WP structure, enhancing its adsorption at the interface of HIPEs droplets and with altered rheological properties. These findings would offer valuable insights for WP modification and its potential applications in HIPEs.
format Article
id doaj-art-1a7796f485a64eadb8d5d488fdf07235
institution DOAJ
issn 2590-1575
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Food Chemistry: X
spelling doaj-art-1a7796f485a64eadb8d5d488fdf072352025-08-20T03:10:00ZengElsevierFood Chemistry: X2590-15752025-04-012710250810.1016/j.fochx.2025.102508Physically modification treatments improved high internal phase emulsions stabilized by walnut proteinMin Yang0Yunkun Zhu1Minwei Zhang2Youning Zhang3Zhongkai Zhao4Liang Wang5Jie Yang6Jun Liu7Yanan Qin8Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing 100083, China; Corresponding authors.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China; Corresponding authors.Walnut protein (WP)-based emulsions are notable for their sustainability and biocompatibility. However, their emulsifying capability is often hindered by a densely packed structure. This study investigates the impact of physical modifications, specifically high-speed shearing, heat treatment, and ultrasonic treatment, on the structure of WP and examines the subsequent effects on the physicochemical properties and stability of the resulting high internal phase emulsions (HIPEs). The three modification methods reduced the average WP particle diameter by 55.38 %, 48.31 %, and 73.66 %, respectively, the corresponding D4,3 values of HIPEs decreased by 41.31 %, 36.77 %, and 50.43 %. Additionally, the gel-like properties and stability of the HIPEs from modified WP significantly improved. Notably, ultrasonic treatment exhibited the most substantial influence, as cavitation effectively unfolded the WP structure, enhancing its adsorption at the interface of HIPEs droplets and with altered rheological properties. These findings would offer valuable insights for WP modification and its potential applications in HIPEs.http://www.sciencedirect.com/science/article/pii/S2590157525003554Walnut proteinProtein structureHigh internal phase emulsionStability
spellingShingle Min Yang
Yunkun Zhu
Minwei Zhang
Youning Zhang
Zhongkai Zhao
Liang Wang
Jie Yang
Jun Liu
Yanan Qin
Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
Food Chemistry: X
Walnut protein
Protein structure
High internal phase emulsion
Stability
title Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
title_full Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
title_fullStr Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
title_full_unstemmed Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
title_short Physically modification treatments improved high internal phase emulsions stabilized by walnut protein
title_sort physically modification treatments improved high internal phase emulsions stabilized by walnut protein
topic Walnut protein
Protein structure
High internal phase emulsion
Stability
url http://www.sciencedirect.com/science/article/pii/S2590157525003554
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