Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study

Abstract Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) is associated with the transient activation of a systemic inflammatory response. Fibronectin (FN), an endogenous inflammatory mediator, is a key component of the extracellular matrix. This study aimed to detect changes...

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Main Authors: Anna Lemańska-Perek, Dorota Krzyżanowska-Gołąb, Grzegorz Wysoczański, Barbara Barteczko-Grajek, Waldemar Goździk, Barbara Adamik
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-80765-9
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author Anna Lemańska-Perek
Dorota Krzyżanowska-Gołąb
Grzegorz Wysoczański
Barbara Barteczko-Grajek
Waldemar Goździk
Barbara Adamik
author_facet Anna Lemańska-Perek
Dorota Krzyżanowska-Gołąb
Grzegorz Wysoczański
Barbara Barteczko-Grajek
Waldemar Goździk
Barbara Adamik
author_sort Anna Lemańska-Perek
collection DOAJ
description Abstract Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) is associated with the transient activation of a systemic inflammatory response. Fibronectin (FN), an endogenous inflammatory mediator, is a key component of the extracellular matrix. This study aimed to detect changes in cellular and plasma FN levels, as well as its potential fragmentation or FN-fibrin complex formation, in 40 patients undergoing CABG with CPB. Our results indicate that CPB was associated with changes in the levels of cellular and plasma FN and with intensified FN fragmentation. Moreover, FN-fibrin complexes were detected in all patients, indicating activation of the coagulation process during CPB. In a multivariate regression analysis, a history of arterial hypertension and CPB duration influenced plasma FN levels at 6 h (β = -0.458, p = 0.001; -0.375, p = 0.008, respectively) and 12 h (β = -0.293, p = 0.026; -0.554, p = 0.000) after surgery. Alterations in FN concentration, intensified FN degradation, and the presence of FN-fibrin complexes after surgery may suggest that these changes are related to the remodelling of the extracellular matrix resulting from cardiac surgery and the associated repair processes. The results indicate that FN has clinical potential as a marker of repair processes.
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spelling doaj-art-1a6bdaa96cfc44ada07b7b39c87b738b2025-08-20T02:43:24ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-80765-9Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational studyAnna Lemańska-Perek0Dorota Krzyżanowska-Gołąb1Grzegorz Wysoczański2Barbara Barteczko-Grajek3Waldemar Goździk4Barbara Adamik5Department of Chemistry and Immunochemistry, Wroclaw Medical UniversityDepartment of Chemistry and Immunochemistry, Wroclaw Medical UniversityUniversity Clinical HospitalClinical Department of Anesthesiology and Intensive Therapy, Wroclaw Medical UniversityClinical Department of Anesthesiology and Intensive Therapy, Wroclaw Medical UniversityClinical Department of Anesthesiology and Intensive Therapy, Wroclaw Medical UniversityAbstract Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) is associated with the transient activation of a systemic inflammatory response. Fibronectin (FN), an endogenous inflammatory mediator, is a key component of the extracellular matrix. This study aimed to detect changes in cellular and plasma FN levels, as well as its potential fragmentation or FN-fibrin complex formation, in 40 patients undergoing CABG with CPB. Our results indicate that CPB was associated with changes in the levels of cellular and plasma FN and with intensified FN fragmentation. Moreover, FN-fibrin complexes were detected in all patients, indicating activation of the coagulation process during CPB. In a multivariate regression analysis, a history of arterial hypertension and CPB duration influenced plasma FN levels at 6 h (β = -0.458, p = 0.001; -0.375, p = 0.008, respectively) and 12 h (β = -0.293, p = 0.026; -0.554, p = 0.000) after surgery. Alterations in FN concentration, intensified FN degradation, and the presence of FN-fibrin complexes after surgery may suggest that these changes are related to the remodelling of the extracellular matrix resulting from cardiac surgery and the associated repair processes. The results indicate that FN has clinical potential as a marker of repair processes.https://doi.org/10.1038/s41598-024-80765-9FibronectinEDA-FNFN-fibrin complexesCardiopulmonary bypassInflammation
spellingShingle Anna Lemańska-Perek
Dorota Krzyżanowska-Gołąb
Grzegorz Wysoczański
Barbara Barteczko-Grajek
Waldemar Goździk
Barbara Adamik
Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
Scientific Reports
Fibronectin
EDA-FN
FN-fibrin complexes
Cardiopulmonary bypass
Inflammation
title Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
title_full Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
title_fullStr Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
title_full_unstemmed Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
title_short Changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass – a prospective, observational study
title_sort changes in various forms of fibronectin in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass a prospective observational study
topic Fibronectin
EDA-FN
FN-fibrin complexes
Cardiopulmonary bypass
Inflammation
url https://doi.org/10.1038/s41598-024-80765-9
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