Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids
Background: Vanilla planifolia and V. pompona exhibit low seed germination rates. Although their fruits are obtained through manual self-pollination, the effect of intra- and interspecific cross-pollination on their fruits and seeds remains unknown. Hybrids organisms are considered potential altern...
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Sociedad Botánica de México, A. C.
2025-06-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3679 |
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| author | José Martín Barreda-Castillo Emerson Ricardo Pansarin Juan L. Monribot-Villanueva Miguel Ángel Lozano-Rodríguez José A. Guerrero-Analco Rebeca Alicia Menchaca-García |
| author_facet | José Martín Barreda-Castillo Emerson Ricardo Pansarin Juan L. Monribot-Villanueva Miguel Ángel Lozano-Rodríguez José A. Guerrero-Analco Rebeca Alicia Menchaca-García |
| author_sort | José Martín Barreda-Castillo |
| collection | DOAJ |
| description |
Background: Vanilla planifolia and V. pompona exhibit low seed germination rates. Although their fruits are obtained through manual self-pollination, the effect of intra- and interspecific cross-pollination on their fruits and seeds remains unknown. Hybrids organisms are considered potential alternatives to traditional cultivation.
Questions: How does cross-pollination affect germination in V. planifolia and V. pompona? Do hybrids seeds exhibit greater germination than their parental species?
Studied species: Vanilla planifolia, V. pompona, V. planifolia × V. pompona, V. pompona × V. planifolia (Orchidaceae)
Study site and dates: Mesa de Guadalupe, Alto Lucero, and La Concepción, Jilotepec, Veracruz, Mexico, in April 2024.
Methods: Manual pollinations (self-pollination, geitonogamy, and metaxenia/xenia) were performed, and fruits from natural pollination were collected. Hybrids were only self-pollinated. Fruits and seeds were characterized, and seeds were planted in vitro under a 16:8 hours light-dark photoperiod, and total darkness.
Results: Higher germination values occurred in natural pollination (67.83 % in V. planifolia and 44.66 % in V. pompona) and metaxenia/xenia (63.2 and 51.66 %, respectively), along with larger fruits and seeds. Geitonogamy exhibited intermediate results (24.46 and 22.79 %, respectively), while self-pollination resulted in the lowest values (4.82 % in V. planifolia and none in V. pompona). Hybrids produced larger fruits, but their seeds did not germinate. Light exposure enhanced germination.
Conclusions: Intra- and interspecific cross pollination promotes germination in vanilla. Hybrid fruits could represent a commercial alternative to traditional vanilla crop.
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| format | Article |
| id | doaj-art-1a6a3e785f5f4ea8829e89061d0d3e1a |
| institution | OA Journals |
| issn | 2007-4298 2007-4476 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj-art-1a6a3e785f5f4ea8829e89061d0d3e1a2025-08-20T02:38:01ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762025-06-01103310.17129/botsci.3679Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybridsJosé Martín Barreda-Castillo0https://orcid.org/0000-0002-1698-7669Emerson Ricardo Pansarin1https://orcid.org/0000-0002-0355-8363Juan L. Monribot-Villanueva2https://orcid.org/0000-0003-2394-0524Miguel Ángel Lozano-Rodríguez3https://orcid.org/0000-0003-0666-1822José A. Guerrero-Analco4https://orcid.org/0000-0003-0998-757XRebeca Alicia Menchaca-García5https://orcid.org/0000-0002-4241-8304Red de Estudios Moleculares Avanzados, Instituto de Ecología (INECOL) A.C., Xalapa, VeracruzDepartamento de Biologia, Faculdade de Filosofia, Ciências e Letras, Universidade de São Paulo, Ribeirão Preto, São PauloRed de Estudios Moleculares Avanzados, Instituto de Ecología (INECOL) A.C., Xalapa, VeracruzFacultad de Ciencias Agrícolas, Universidad Veracruzana, Xalapa, VeracruzRed de Estudios Moleculares Avanzados, Instituto de Ecología (INECOL) A.C., Xalapa, VeracruzCentro de Investigaciones Tropicales (CITRO), Universidad Veracruzana, Xalapa, Veracruz Background: Vanilla planifolia and V. pompona exhibit low seed germination rates. Although their fruits are obtained through manual self-pollination, the effect of intra- and interspecific cross-pollination on their fruits and seeds remains unknown. Hybrids organisms are considered potential alternatives to traditional cultivation. Questions: How does cross-pollination affect germination in V. planifolia and V. pompona? Do hybrids seeds exhibit greater germination than their parental species? Studied species: Vanilla planifolia, V. pompona, V. planifolia × V. pompona, V. pompona × V. planifolia (Orchidaceae) Study site and dates: Mesa de Guadalupe, Alto Lucero, and La Concepción, Jilotepec, Veracruz, Mexico, in April 2024. Methods: Manual pollinations (self-pollination, geitonogamy, and metaxenia/xenia) were performed, and fruits from natural pollination were collected. Hybrids were only self-pollinated. Fruits and seeds were characterized, and seeds were planted in vitro under a 16:8 hours light-dark photoperiod, and total darkness. Results: Higher germination values occurred in natural pollination (67.83 % in V. planifolia and 44.66 % in V. pompona) and metaxenia/xenia (63.2 and 51.66 %, respectively), along with larger fruits and seeds. Geitonogamy exhibited intermediate results (24.46 and 22.79 %, respectively), while self-pollination resulted in the lowest values (4.82 % in V. planifolia and none in V. pompona). Hybrids produced larger fruits, but their seeds did not germinate. Light exposure enhanced germination. Conclusions: Intra- and interspecific cross pollination promotes germination in vanilla. Hybrid fruits could represent a commercial alternative to traditional vanilla crop. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3679GeitonogamyMetaxeniaNatural pollinationSelf-pollinationxenia |
| spellingShingle | José Martín Barreda-Castillo Emerson Ricardo Pansarin Juan L. Monribot-Villanueva Miguel Ángel Lozano-Rodríguez José A. Guerrero-Analco Rebeca Alicia Menchaca-García Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids Botanical Sciences Geitonogamy Metaxenia Natural pollination Self-pollination xenia |
| title | Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids |
| title_full | Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids |
| title_fullStr | Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids |
| title_full_unstemmed | Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids |
| title_short | Effect of pollen source on germination of Vanilla planifolia, V. pompona (Orchidaceae), and their interspecific hybrids |
| title_sort | effect of pollen source on germination of vanilla planifolia v pompona orchidaceae and their interspecific hybrids |
| topic | Geitonogamy Metaxenia Natural pollination Self-pollination xenia |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3679 |
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