Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance
Cyclin‐dependent kinase 1 (CDK1) plays a significant role in certain malignancies. However, it remains unclear whether CDK1 plays a role in esophageal squamous cell carcinoma (ESCC). The aim of this study was to analyze the expression and clinical value of CDK1 in ESCC. CDK1 protein in 151 ESCC tiss...
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| Format: | Article |
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Wiley
2021-11-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13306 |
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| author | Han‐Jie Zhang Gang Chen Shang‐Wei Chen Zong‐Wang Fu Hua‐Fu Zhou Zhen‐Bo Feng Jun‐Xian Mo Chang‐Bo Li Jun Liu |
| author_facet | Han‐Jie Zhang Gang Chen Shang‐Wei Chen Zong‐Wang Fu Hua‐Fu Zhou Zhen‐Bo Feng Jun‐Xian Mo Chang‐Bo Li Jun Liu |
| author_sort | Han‐Jie Zhang |
| collection | DOAJ |
| description | Cyclin‐dependent kinase 1 (CDK1) plays a significant role in certain malignancies. However, it remains unclear whether CDK1 plays a role in esophageal squamous cell carcinoma (ESCC). The aim of this study was to analyze the expression and clinical value of CDK1 in ESCC. CDK1 protein in 151 ESCC tissues and 138 normal esophageal tissues was detected by immunohistochemistry. RNA‐seq of eight pairs of ESCC and adjacent esophageal specimens was performed to evaluate the levels of CDK1 mRNA. Microarray and external RNA‐seq data from 664 cases of ESCC and 1733 cases of control tissues were used to verify the difference in CDK1 expression between the two groups. A comprehensive analysis of all data was performed to evaluate the difference in CDK1 between ESCC tissues and control tissues. Further, functional enrichment analyses were performed based on differentially expressed genes (DEGs) of ESCC and co‐expressed genes (CEGs) of CDK1. In addition, a lncRNA‐miRNA‐CDK1 network was constructed. The expression of CDK1 protein was obviously increased in ESCC tissues (3.540 ± 2.923 vs. 1.040 ± 1.632, P < 0.001). RNA‐seq indicated that the mRNA level of CDK1 was also highly expressed in ESCC tissues (5.261 ± 0.703 vs. 2.229 ± 1.161, P < 0.0001). Comprehensive analysis revealed consistent up‐regulation of CDK1 (SMD = 1.41; 95% CI 1.00–1.83). Further, functional enrichment analyses revealed that the functions of these genes were mainly concentrated in the cell cycle. A triple regulatory network of PVT1‐hsa‐miR‐145‐5p/hsa‐miR‐30c‐5p‐CDK1 was constructed using in silico analysis. In summary, overexpression of CDK1 is closely related to ESCC tumorigenesis. |
| format | Article |
| id | doaj-art-1a6741172cf742ea8efa58965a0cc344 |
| institution | DOAJ |
| issn | 2211-5463 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-1a6741172cf742ea8efa58965a0cc3442025-08-20T02:58:19ZengWileyFEBS Open Bio2211-54632021-11-0111113126314110.1002/2211-5463.13306Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significanceHan‐Jie Zhang0Gang Chen1Shang‐Wei Chen2Zong‐Wang Fu3Hua‐Fu Zhou4Zhen‐Bo Feng5Jun‐Xian Mo6Chang‐Bo Li7Jun Liu8Department of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Cardio‐Thoracic Surgery The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Cardio‐Thoracic Surgery The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Cardio‐Thoracic Surgery The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Pathology The First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Cardio‐Thoracic Surgery The Seventh Affiliated Hospital of Guangxi Medical University Wuzhou ChinaDepartment of Cardio‐Thoracic Surgery The Seventh Affiliated Hospital of Guangxi Medical University Wuzhou ChinaDepartment of Cardio‐Thoracic Surgery The First Affiliated Hospital of Guangxi Medical University Nanning ChinaCyclin‐dependent kinase 1 (CDK1) plays a significant role in certain malignancies. However, it remains unclear whether CDK1 plays a role in esophageal squamous cell carcinoma (ESCC). The aim of this study was to analyze the expression and clinical value of CDK1 in ESCC. CDK1 protein in 151 ESCC tissues and 138 normal esophageal tissues was detected by immunohistochemistry. RNA‐seq of eight pairs of ESCC and adjacent esophageal specimens was performed to evaluate the levels of CDK1 mRNA. Microarray and external RNA‐seq data from 664 cases of ESCC and 1733 cases of control tissues were used to verify the difference in CDK1 expression between the two groups. A comprehensive analysis of all data was performed to evaluate the difference in CDK1 between ESCC tissues and control tissues. Further, functional enrichment analyses were performed based on differentially expressed genes (DEGs) of ESCC and co‐expressed genes (CEGs) of CDK1. In addition, a lncRNA‐miRNA‐CDK1 network was constructed. The expression of CDK1 protein was obviously increased in ESCC tissues (3.540 ± 2.923 vs. 1.040 ± 1.632, P < 0.001). RNA‐seq indicated that the mRNA level of CDK1 was also highly expressed in ESCC tissues (5.261 ± 0.703 vs. 2.229 ± 1.161, P < 0.0001). Comprehensive analysis revealed consistent up‐regulation of CDK1 (SMD = 1.41; 95% CI 1.00–1.83). Further, functional enrichment analyses revealed that the functions of these genes were mainly concentrated in the cell cycle. A triple regulatory network of PVT1‐hsa‐miR‐145‐5p/hsa‐miR‐30c‐5p‐CDK1 was constructed using in silico analysis. In summary, overexpression of CDK1 is closely related to ESCC tumorigenesis.https://doi.org/10.1002/2211-5463.13306cyclin dependent kinase 1esophageal squamous cell carcinomaimmunohistochemistryRNA‐seq |
| spellingShingle | Han‐Jie Zhang Gang Chen Shang‐Wei Chen Zong‐Wang Fu Hua‐Fu Zhou Zhen‐Bo Feng Jun‐Xian Mo Chang‐Bo Li Jun Liu Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance FEBS Open Bio cyclin dependent kinase 1 esophageal squamous cell carcinoma immunohistochemistry RNA‐seq |
| title | Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| title_full | Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| title_fullStr | Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| title_full_unstemmed | Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| title_short | Overexpression of cyclin‐dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| title_sort | overexpression of cyclin dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance |
| topic | cyclin dependent kinase 1 esophageal squamous cell carcinoma immunohistochemistry RNA‐seq |
| url | https://doi.org/10.1002/2211-5463.13306 |
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