The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)
Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal...
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/2808241 |
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| author | Márcia Regina Ribeiro Alves Abraham Damian Giraldo Zuñiga Rita de Cássia Superbi Sousa Carmelita Zacchi Scolforo |
| author_facet | Márcia Regina Ribeiro Alves Abraham Damian Giraldo Zuñiga Rita de Cássia Superbi Sousa Carmelita Zacchi Scolforo |
| author_sort | Márcia Regina Ribeiro Alves |
| collection | DOAJ |
| description | Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (where qm=87.95 mg g−1 and 74.2%; 68.26 mg g−1 and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively. |
| format | Article |
| id | doaj-art-1a581aaf7c734993b2c9e9bcb1dbab47 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-1a581aaf7c734993b2c9e9bcb1dbab472025-08-20T02:01:57ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/28082412808241The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)Márcia Regina Ribeiro Alves0Abraham Damian Giraldo Zuñiga1Rita de Cássia Superbi Sousa2Carmelita Zacchi Scolforo3Biodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, BrazilBiodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, BrazilDepartment of Chemistry, Federal University of Viçosa, Avenida PH Holfs, 36570-000 Viçosa, MG, BrazilDepartment of Nutrition, Federal University of Espírito Santo, Avenida Fernando Ferrari, No. 514, Goiabeiras, 29075-010 Vitória, ES, BrazilBovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (where qm=87.95 mg g−1 and 74.2%; 68.26 mg g−1 and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.http://dx.doi.org/10.1155/2016/2808241 |
| spellingShingle | Márcia Regina Ribeiro Alves Abraham Damian Giraldo Zuñiga Rita de Cássia Superbi Sousa Carmelita Zacchi Scolforo The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) The Scientific World Journal |
| title | The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) |
| title_full | The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) |
| title_fullStr | The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) |
| title_full_unstemmed | The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) |
| title_short | The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana) |
| title_sort | process of separating bovine serum albumin using hydroxyapatite and active babassu coal orbignya martiana |
| url | http://dx.doi.org/10.1155/2016/2808241 |
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