Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba
Abstract Fusarium wilt causes destructive effects on Vicia faba (VF). This study is innovative because it evaluates the comparative effects of various biological treatments on different VF cultivars to determine the therapeutic potential of each treatment against Fusarium wilt. In this study, the su...
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Nature Portfolio
2025-05-01
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| author | Mohamed S. Attia Deiaa A. El-Wakil Amr H. Hashem Abdulaziz A. Al-Askar Hamada AbdElgawad Rawan Saif Alotaibi Saad. A. Abdel-Kader Amer M. Abdelaziz |
| author_facet | Mohamed S. Attia Deiaa A. El-Wakil Amr H. Hashem Abdulaziz A. Al-Askar Hamada AbdElgawad Rawan Saif Alotaibi Saad. A. Abdel-Kader Amer M. Abdelaziz |
| author_sort | Mohamed S. Attia |
| collection | DOAJ |
| description | Abstract Fusarium wilt causes destructive effects on Vicia faba (VF). This study is innovative because it evaluates the comparative effects of various biological treatments on different VF cultivars to determine the therapeutic potential of each treatment against Fusarium wilt. In this study, the suppression of F. verticillioides (FV) by Bacillus subtilis and Trichoderma harzianum was compared at both the pot level and in vitro. The findings indicated that both B. subtilis and T. harzianum have antifungal effects on F. verticillioides in vitro, exhibiting mycelium inhibition percentages of 45.88% and 68.20%. Our results showed that treatments with T. harzianum significantly reduced the disease incidence (DI) in both the V. faba ‘Giza-716’ (G) and ‘Sakha-101’ (S) cultivars, providing comparable protection at 70.58% and 72.22%, respectively. After the afflicted plant received biological therapies, a notable increase in all yield components was observed. The G and S cultivars treated with T. harzianum exhibited the highest PPO activity, measuring 116.70% and 227.70%. The S cultivar treated with T. harzianum showed the highest POD activity at 289.60%. In conclusion, T. harzianum and B. subtilis as potential bio pesticides exhibited high efficacy against F. verticillioides, which causes wilt in several Vicia faba cultivars, due to their effectiveness and environmental friendliness. |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-1a579a2f660d49ca83dc96574a7e16c22025-08-20T03:53:12ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-99381-2Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia fabaMohamed S. Attia0Deiaa A. El-Wakil1Amr H. Hashem2Abdulaziz A. Al-Askar3Hamada AbdElgawad4Rawan Saif Alotaibi5Saad. A. Abdel-Kader6Amer M. Abdelaziz7Department of Botany and Microbiology, Faculty of Science, Al-Azhar UniversitySeed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research CenterDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar UniversityDepartment of Botany and Microbiology, Faculty of Science, King Saud UniversityIntegrated Molecular Plant Physiology Research, Department of Biology, University of AntwerpDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityPlant Protection Department, Faculty of Agriculture, Al-Azhar UniversityDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar UniversityAbstract Fusarium wilt causes destructive effects on Vicia faba (VF). This study is innovative because it evaluates the comparative effects of various biological treatments on different VF cultivars to determine the therapeutic potential of each treatment against Fusarium wilt. In this study, the suppression of F. verticillioides (FV) by Bacillus subtilis and Trichoderma harzianum was compared at both the pot level and in vitro. The findings indicated that both B. subtilis and T. harzianum have antifungal effects on F. verticillioides in vitro, exhibiting mycelium inhibition percentages of 45.88% and 68.20%. Our results showed that treatments with T. harzianum significantly reduced the disease incidence (DI) in both the V. faba ‘Giza-716’ (G) and ‘Sakha-101’ (S) cultivars, providing comparable protection at 70.58% and 72.22%, respectively. After the afflicted plant received biological therapies, a notable increase in all yield components was observed. The G and S cultivars treated with T. harzianum exhibited the highest PPO activity, measuring 116.70% and 227.70%. The S cultivar treated with T. harzianum showed the highest POD activity at 289.60%. In conclusion, T. harzianum and B. subtilis as potential bio pesticides exhibited high efficacy against F. verticillioides, which causes wilt in several Vicia faba cultivars, due to their effectiveness and environmental friendliness.https://doi.org/10.1038/s41598-025-99381-2FusariumVicia fabaTrichodermaBiocontrolFungicidal |
| spellingShingle | Mohamed S. Attia Deiaa A. El-Wakil Amr H. Hashem Abdulaziz A. Al-Askar Hamada AbdElgawad Rawan Saif Alotaibi Saad. A. Abdel-Kader Amer M. Abdelaziz Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba Scientific Reports Fusarium Vicia faba Trichoderma Biocontrol Fungicidal |
| title | Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba |
| title_full | Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba |
| title_fullStr | Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba |
| title_full_unstemmed | Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba |
| title_short | Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba |
| title_sort | investigating the activity of bacillus subtilis and trichoderma harzianum to mitigate fusarium wilt disease of diverse cultivars of vicia faba |
| topic | Fusarium Vicia faba Trichoderma Biocontrol Fungicidal |
| url | https://doi.org/10.1038/s41598-025-99381-2 |
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