Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells
Abstract Transplanted adipose stem cells (ASC) have a low survival rate in the body, and there are not many ASC that can be effectively used, which weakens their tissue repair function. Based on this status quo, a new type of copper‐based metal–organic network (Cu‐MON) was used to pretreat cells to...
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | FASEB BioAdvances |
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| Online Access: | https://doi.org/10.1096/fba.2024-00153 |
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| author | Ruixin Pang Kaijing Liu Biou Liu Jing Yang |
| author_facet | Ruixin Pang Kaijing Liu Biou Liu Jing Yang |
| author_sort | Ruixin Pang |
| collection | DOAJ |
| description | Abstract Transplanted adipose stem cells (ASC) have a low survival rate in the body, and there are not many ASC that can be effectively used, which weakens their tissue repair function. Based on this status quo, a new type of copper‐based metal–organic network (Cu‐MON) was used to pretreat cells to regulate cell activity in order to improve the efficacy of cell therapy or reduce the number of cells used, thus reducing the cost of clinical treatment. Gene expression changes before and after Cu‐MON treatment of normal donor adipose stem cells (ND‐ASC) and type 2 diabetes mellitus adipose stem cells (T2DM‐ASC) were evaluated through RNA sequencing, KEGG and GO enrichment analysis. The results showed that Cu‐MON improved ASC cell quality by regulating immune response and promoting paracrine secretion. IL‐17 signaling pathway and IL‐6, CXCL8, and MMP‐9 were key pathways and necessary genes that affected the ability of stem cells. In addition, Cu‐MON also improved stem cell antiviral ability through Type I interferon signaling pathway. Our research showed that Cu‐MON had improved the cell quality of ASC by regulating immune response, promoting paracrine secretion, and improving antiviral capabilities. This approach to biomaterial pretreatment is fast, convenient, and relatively safe, and provides new strategies for improving the efficiency of cell therapies. |
| format | Article |
| id | doaj-art-1a4236fb0c064b1bb3fd3e15edd25d0d |
| institution | DOAJ |
| issn | 2573-9832 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | FASEB BioAdvances |
| spelling | doaj-art-1a4236fb0c064b1bb3fd3e15edd25d0d2025-08-20T03:02:11ZengWileyFASEB BioAdvances2573-98322025-03-0173n/an/a10.1096/fba.2024-00153Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cellsRuixin Pang0Kaijing Liu1Biou Liu2Jing Yang3Fourth Clinical Medical College & Fourth Affiliated Hospital of Harbin Medical University Harbin ChinaState Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering Chinese Academy of Medical Science & Peking Union Medical College Tianjin ChinaDepartment of General Surgery Forth Affiliate Hospital of Harbin Medical University Harbin ChinaState Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering Chinese Academy of Medical Science & Peking Union Medical College Tianjin ChinaAbstract Transplanted adipose stem cells (ASC) have a low survival rate in the body, and there are not many ASC that can be effectively used, which weakens their tissue repair function. Based on this status quo, a new type of copper‐based metal–organic network (Cu‐MON) was used to pretreat cells to regulate cell activity in order to improve the efficacy of cell therapy or reduce the number of cells used, thus reducing the cost of clinical treatment. Gene expression changes before and after Cu‐MON treatment of normal donor adipose stem cells (ND‐ASC) and type 2 diabetes mellitus adipose stem cells (T2DM‐ASC) were evaluated through RNA sequencing, KEGG and GO enrichment analysis. The results showed that Cu‐MON improved ASC cell quality by regulating immune response and promoting paracrine secretion. IL‐17 signaling pathway and IL‐6, CXCL8, and MMP‐9 were key pathways and necessary genes that affected the ability of stem cells. In addition, Cu‐MON also improved stem cell antiviral ability through Type I interferon signaling pathway. Our research showed that Cu‐MON had improved the cell quality of ASC by regulating immune response, promoting paracrine secretion, and improving antiviral capabilities. This approach to biomaterial pretreatment is fast, convenient, and relatively safe, and provides new strategies for improving the efficiency of cell therapies.https://doi.org/10.1096/fba.2024-00153gene expressionRNA sequence analysisstem cellstranscriptometype 2 diabetes mellitus |
| spellingShingle | Ruixin Pang Kaijing Liu Biou Liu Jing Yang Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells FASEB BioAdvances gene expression RNA sequence analysis stem cells transcriptome type 2 diabetes mellitus |
| title | Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| title_full | Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| title_fullStr | Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| title_full_unstemmed | Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| title_short | Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| title_sort | enhancement of adipose stem cell quality via cu mon transcriptome and bioinformatics analysis of normal and diabetic stem cells |
| topic | gene expression RNA sequence analysis stem cells transcriptome type 2 diabetes mellitus |
| url | https://doi.org/10.1096/fba.2024-00153 |
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