Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer
Saliva has already proven to be a prospective diagnostic bioresource for both early disease detection and timely intervention due to its easy accessibility, noninvasiveness, and reproducibility. However, the in-depth identification of salivary proteins needs to be further improved. Until now, only 3...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | IET Nanobiotechnology |
| Online Access: | http://dx.doi.org/10.1049/nbt2/6616433 |
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| author | Lingling Jiao Huilin Dong Changjian Wu Jing Liu Chenhui Wang Yi Cao Fan Cao Ying Zhu Huaiyuan Zhu |
| author_facet | Lingling Jiao Huilin Dong Changjian Wu Jing Liu Chenhui Wang Yi Cao Fan Cao Ying Zhu Huaiyuan Zhu |
| author_sort | Lingling Jiao |
| collection | DOAJ |
| description | Saliva has already proven to be a prospective diagnostic bioresource for both early disease detection and timely intervention due to its easy accessibility, noninvasiveness, and reproducibility. However, the in-depth identification of salivary proteins needs to be further improved. Until now, only 3427 proteins are included in the human salivary proteome (HSP), which is far from the millions of proteins that make up humans. Here, we set out to quantitatively map the HSP in rapid and in-depth Orbitrap Astral mass spectrometer (MS) and coronal nanomagnetic bead–based proteomics workflow. Our study reported 5937 salivary proteins, which was about 73% more than that recorded in HSP. Moreover, we compared the differences between the young and aged salivary proteins. The predominant functions of the upregulated proteins in the young were related to motor proteins and cardiomyopathy, whereas those of the aged were primarily upregulated with oxidation reaction, as well as neurodegenerative disorders. It is the first study to carry out salivary proteomics using a fast and deep Orbitrap Astral MS and remarkably enlarged the number of proteins with HSP, furthermore, salivary proteomics was found to be characterized in the young and aged. With the rapidly advancing MS and proteomics technologies, we believe that salivary protein biomarkers will be more promising for clinical diagnosis and prognosis of human diseases in the future. |
| format | Article |
| id | doaj-art-1a3fbcb4ee304be2be9611882a1d20b3 |
| institution | DOAJ |
| issn | 1751-875X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-1a3fbcb4ee304be2be9611882a1d20b32025-08-20T03:12:27ZengWileyIET Nanobiotechnology1751-875X2025-01-01202510.1049/nbt2/6616433Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass SpectrometerLingling Jiao0Huilin Dong1Changjian Wu2Jing Liu3Chenhui Wang4Yi Cao5Fan Cao6Ying Zhu7Huaiyuan Zhu8China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.China Tobacco Jiangsu Industrial Co. Ltd.Saliva has already proven to be a prospective diagnostic bioresource for both early disease detection and timely intervention due to its easy accessibility, noninvasiveness, and reproducibility. However, the in-depth identification of salivary proteins needs to be further improved. Until now, only 3427 proteins are included in the human salivary proteome (HSP), which is far from the millions of proteins that make up humans. Here, we set out to quantitatively map the HSP in rapid and in-depth Orbitrap Astral mass spectrometer (MS) and coronal nanomagnetic bead–based proteomics workflow. Our study reported 5937 salivary proteins, which was about 73% more than that recorded in HSP. Moreover, we compared the differences between the young and aged salivary proteins. The predominant functions of the upregulated proteins in the young were related to motor proteins and cardiomyopathy, whereas those of the aged were primarily upregulated with oxidation reaction, as well as neurodegenerative disorders. It is the first study to carry out salivary proteomics using a fast and deep Orbitrap Astral MS and remarkably enlarged the number of proteins with HSP, furthermore, salivary proteomics was found to be characterized in the young and aged. With the rapidly advancing MS and proteomics technologies, we believe that salivary protein biomarkers will be more promising for clinical diagnosis and prognosis of human diseases in the future.http://dx.doi.org/10.1049/nbt2/6616433 |
| spellingShingle | Lingling Jiao Huilin Dong Changjian Wu Jing Liu Chenhui Wang Yi Cao Fan Cao Ying Zhu Huaiyuan Zhu Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer IET Nanobiotechnology |
| title | Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer |
| title_full | Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer |
| title_fullStr | Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer |
| title_full_unstemmed | Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer |
| title_short | Ultrafast and Deep Saliva Proteome Reveals the Dynamic of Human Saliva With Aging by Orbitrap Astral Mass Spectrometer |
| title_sort | ultrafast and deep saliva proteome reveals the dynamic of human saliva with aging by orbitrap astral mass spectrometer |
| url | http://dx.doi.org/10.1049/nbt2/6616433 |
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