HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway
ObjectiveTo investigate the molecular mechanism by which HMGA2 participates in the TGF-β/Smad pathway in the regulation of the proliferation, aggression, and metastasis of laryngeal cancer. MethodsshRNA transfection was used to construct the HMGA2 knockdown laryngeal cancer TU686 cell model, and sub...
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Magazine House of Cancer Research on Prevention and Treatment
2025-07-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1216 |
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| _version_ | 1850078011677736960 |
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| author | Xianxue WEN Ruting LI Xi WU Renbin GUO Jun WU Lijuan MA |
| author_facet | Xianxue WEN Ruting LI Xi WU Renbin GUO Jun WU Lijuan MA |
| author_sort | Xianxue WEN |
| collection | DOAJ |
| description | ObjectiveTo investigate the molecular mechanism by which HMGA2 participates in the TGF-β/Smad pathway in the regulation of the proliferation, aggression, and metastasis of laryngeal cancer. MethodsshRNA transfection was used to construct the HMGA2 knockdown laryngeal cancer TU686 cell model, and subcutaneous transplantation tumor model and tail vein metastasis tumor model were established in nude mice. Western blot was conducted to detect the expression of HMGA2 and TGF-β/Smad pathway-related molecules in cells and tumor tissues. ResultsThe proliferation, invasion, and metastasis of TU686 cells with HMGA2 knockdown decreased. The expression of TGF-β, Smad2, Smad3, and phosphorylated Smad2/3 protein also decreased. TGF-β1 stimulation of the TGF-β/Smad pathway could partially offset the antitumor effect caused by HMGA2 knockdown. Through in vitro experiments, we determined that low expression of HMGA2 significantly inhibited the growth of subcutaneously transplanted tumors, and TGF-β1 stimulation of the TGF-β/Smad pathway reduced the tumor-inhibitory effect resulting from the low expression of HMGA2. In tail vein metastases of nude mice, E-cadherin expression was elevated but N-cadherin expression was reduced in the HMGA2 knockdown group, suggesting that HMGA2 could inhibit the progression of EMT. After TGF-β1 stimulated the TGF-β/Smad pathway, the EMT effect due to HMGA2 knockdown was lessened. ConclusionHMGA2 may promote the proliferation, invasion, and metastasis of laryngeal cancer by upregulating the TGF-β/Smad signaling pathway. |
| format | Article |
| id | doaj-art-1a1a60b9dfde49febfc69978bf649553 |
| institution | DOAJ |
| issn | 1000-8578 |
| language | zho |
| publishDate | 2025-07-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj-art-1a1a60b9dfde49febfc69978bf6495532025-08-20T02:45:41ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782025-07-0152757157710.3971/j.issn.1000-8578.2025.24.121620241216HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling PathwayXianxue WEN0Ruting LI1Xi WU2Renbin GUO3Jun WU4Lijuan MA5Department of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaDepartment of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaDepartment of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaDepartment of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaDepartment of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaDepartment of Otolaryngology and Head/Neck Surgery, First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha 410005, ChinaObjectiveTo investigate the molecular mechanism by which HMGA2 participates in the TGF-β/Smad pathway in the regulation of the proliferation, aggression, and metastasis of laryngeal cancer. MethodsshRNA transfection was used to construct the HMGA2 knockdown laryngeal cancer TU686 cell model, and subcutaneous transplantation tumor model and tail vein metastasis tumor model were established in nude mice. Western blot was conducted to detect the expression of HMGA2 and TGF-β/Smad pathway-related molecules in cells and tumor tissues. ResultsThe proliferation, invasion, and metastasis of TU686 cells with HMGA2 knockdown decreased. The expression of TGF-β, Smad2, Smad3, and phosphorylated Smad2/3 protein also decreased. TGF-β1 stimulation of the TGF-β/Smad pathway could partially offset the antitumor effect caused by HMGA2 knockdown. Through in vitro experiments, we determined that low expression of HMGA2 significantly inhibited the growth of subcutaneously transplanted tumors, and TGF-β1 stimulation of the TGF-β/Smad pathway reduced the tumor-inhibitory effect resulting from the low expression of HMGA2. In tail vein metastases of nude mice, E-cadherin expression was elevated but N-cadherin expression was reduced in the HMGA2 knockdown group, suggesting that HMGA2 could inhibit the progression of EMT. After TGF-β1 stimulated the TGF-β/Smad pathway, the EMT effect due to HMGA2 knockdown was lessened. ConclusionHMGA2 may promote the proliferation, invasion, and metastasis of laryngeal cancer by upregulating the TGF-β/Smad signaling pathway.http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1216laryngeal cancertu686 cellhmga2tgf-β/smademt |
| spellingShingle | Xianxue WEN Ruting LI Xi WU Renbin GUO Jun WU Lijuan MA HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway Zhongliu Fangzhi Yanjiu laryngeal cancer tu686 cell hmga2 tgf-β/smad emt |
| title | HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway |
| title_full | HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway |
| title_fullStr | HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway |
| title_full_unstemmed | HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway |
| title_short | HMGA2 Promotes Cellular Proliferation, Invasion and Metastasis of Laryngeal Cancer Through TGF-β/Smad Signaling Pathway |
| title_sort | hmga2 promotes cellular proliferation invasion and metastasis of laryngeal cancer through tgf β smad signaling pathway |
| topic | laryngeal cancer tu686 cell hmga2 tgf-β/smad emt |
| url | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1216 |
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