Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis
Ophthalmic mycoses caused by infectious fungi are being recognized as a serious concern since they lead to total blindness. Cephalosporium is one amongst several opportunistic fungal species implicated in ophthalmic infections leading to mycotic keratitis. A mitogenic lectin has been purified from t...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2010/854656 |
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| author | Nagaraja N. Nagre Vishwanath B. Chachadi Sachin M. Eligar C. Shubhada Radha Pujari Padma Shastry Bale M. Swamy Shashikala R. Inamdar |
| author_facet | Nagaraja N. Nagre Vishwanath B. Chachadi Sachin M. Eligar C. Shubhada Radha Pujari Padma Shastry Bale M. Swamy Shashikala R. Inamdar |
| author_sort | Nagaraja N. Nagre |
| collection | DOAJ |
| description | Ophthalmic mycoses caused by infectious fungi are being recognized as a serious concern since they lead to total blindness. Cephalosporium is one amongst several opportunistic fungal species implicated in ophthalmic infections leading to mycotic keratitis. A mitogenic lectin has been purified from the mycelia of fungus Cephalosporium, isolated from the corneal smears of a keratitis patient. Cephalosporium lectin (CSL) is a tetramer with subunit mass of 14 kDa, agglutinates human A, B, and O erythrocytes, and exhibits high affinity for mucin compared to fetuin and asialofetuin but does not bind to simple sugars indicating its complex sugar specificity. CSL showed strong binding to normal
human peripheral blood mononuclear cells (PBMCs) to elicit mitogenic activity. The sugar specificity of the lectin and its interaction with PBMCs to exhibit mitogenic effect indicate its possible role in adhesion and infection process of Cephalosporium. |
| format | Article |
| id | doaj-art-1a19acf81d154e5abfbe5e5d82b87b56 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-1a19acf81d154e5abfbe5e5d82b87b562025-02-03T00:59:06ZengWileyBiochemistry Research International2090-22472090-22552010-01-01201010.1155/2010/854656854656Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic KeratitisNagaraja N. Nagre0Vishwanath B. Chachadi1Sachin M. Eligar2C. Shubhada3Radha Pujari4Padma Shastry5Bale M. Swamy6Shashikala R. Inamdar7Department of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaDepartment of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaDepartment of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaDepartment of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaNational Center for Cell Sciences, Ganeshkhind, Pune 411 007, IndiaNational Center for Cell Sciences, Ganeshkhind, Pune 411 007, IndiaDepartment of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaDepartment of Biochemistry, Karnatak University, Dharwad 580 003, Karnataka, IndiaOphthalmic mycoses caused by infectious fungi are being recognized as a serious concern since they lead to total blindness. Cephalosporium is one amongst several opportunistic fungal species implicated in ophthalmic infections leading to mycotic keratitis. A mitogenic lectin has been purified from the mycelia of fungus Cephalosporium, isolated from the corneal smears of a keratitis patient. Cephalosporium lectin (CSL) is a tetramer with subunit mass of 14 kDa, agglutinates human A, B, and O erythrocytes, and exhibits high affinity for mucin compared to fetuin and asialofetuin but does not bind to simple sugars indicating its complex sugar specificity. CSL showed strong binding to normal human peripheral blood mononuclear cells (PBMCs) to elicit mitogenic activity. The sugar specificity of the lectin and its interaction with PBMCs to exhibit mitogenic effect indicate its possible role in adhesion and infection process of Cephalosporium.http://dx.doi.org/10.1155/2010/854656 |
| spellingShingle | Nagaraja N. Nagre Vishwanath B. Chachadi Sachin M. Eligar C. Shubhada Radha Pujari Padma Shastry Bale M. Swamy Shashikala R. Inamdar Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis Biochemistry Research International |
| title | Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis |
| title_full | Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis |
| title_fullStr | Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis |
| title_full_unstemmed | Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis |
| title_short | Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis |
| title_sort | purification and characterization of a mitogenic lectin from cephalosporium a pathogenic fungus causing mycotic keratitis |
| url | http://dx.doi.org/10.1155/2010/854656 |
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