Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer
Background: Aberrant methylation and expression of various noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), confer a great potential as tumor markers. This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MA...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2025-04-01
|
| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | https://mejc.sums.ac.ir/article_50236_38dc29b1d2af2e7f9a0f8e94d34c4c9a.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849731915397988352 |
|---|---|
| author | Marziyeh Memarzadeh Habib Zarredar Milad Asadi Armin Sadeghi Venus Zafari Shahryar Hashemzadeh Hamed Sabbagh-Jadid Mortaza Raeisi |
| author_facet | Marziyeh Memarzadeh Habib Zarredar Milad Asadi Armin Sadeghi Venus Zafari Shahryar Hashemzadeh Hamed Sabbagh-Jadid Mortaza Raeisi |
| author_sort | Marziyeh Memarzadeh |
| collection | DOAJ |
| description | Background: Aberrant methylation and expression of various noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), confer a great potential as tumor markers. This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MALAT-1) expression patterns as potential diagnostic biomarkers for non-small cell lung cancer (NSCLC).Method: In this cross-sectional study, miR-30b DNA methylation and MALAT-1 expression patterns were first explored using microarray data retrieved from the NSCLC dataset in the Cancer Genome Atlas (TCGA)-LUNG. Then, the obtained results were further validated in internal samples. Subsequently, genomic DNA was extracted and modified by sodium bisulfite to determine DNA methylation using q-MSP. Total RNA was extracted and transcribed to cDNA to measure transcription level by quantitative real-time polymerase chain reaction. GraphPad 6 Prism v.8 was used to perform the statistical analyses. Comparisons between groups in internal samples were conducted by paired student's t-test, while Mann-Whitney U test was used to analyze TCGA-LUNG data (P < 0.05).Results: Our results indicated miR-30b hypermethylation, miR-30b downregulation and lncRNA MALAT-1 overexpression in NSCLC tumor samples compared with marginal normal samples. These changes were significantly associated with the stage of malignancy like lymph node metastasis. Also, using receiver operating characteristic curve analysis, MALAT-1 expression, and miR-30b methylation and expression patterns were found as possible diagnostic biomarkers for NSCLC (area under the curve was 0.70, 0.67, and 0.74, respectively).Conclusion: We found involvement of miR-30b hypermethylation and downregulation as well as lncRNA MALAT-1 overexpression with tumor outcomes of NSCLC patients. |
| format | Article |
| id | doaj-art-1a10fb1d9e334481aa2d54869c6f31a2 |
| institution | DOAJ |
| issn | 2008-6709 2008-6687 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj-art-1a10fb1d9e334481aa2d54869c6f31a22025-08-20T03:08:24ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872025-04-0116212713810.30476/mejc.2024.101524.203550236Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung CancerMarziyeh Memarzadeh0Habib Zarredar1Milad Asadi2Armin Sadeghi3Venus Zafari4Shahryar Hashemzadeh5Hamed Sabbagh-Jadid6Mortaza Raeisi7Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Basic Oncology of Health Institute of Ege University, Izmir, TurkeyTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Basic Oncology of Health Institute of Ege University, Izmir, TurkeyTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranHematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, IranBackground: Aberrant methylation and expression of various noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), confer a great potential as tumor markers. This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MALAT-1) expression patterns as potential diagnostic biomarkers for non-small cell lung cancer (NSCLC).Method: In this cross-sectional study, miR-30b DNA methylation and MALAT-1 expression patterns were first explored using microarray data retrieved from the NSCLC dataset in the Cancer Genome Atlas (TCGA)-LUNG. Then, the obtained results were further validated in internal samples. Subsequently, genomic DNA was extracted and modified by sodium bisulfite to determine DNA methylation using q-MSP. Total RNA was extracted and transcribed to cDNA to measure transcription level by quantitative real-time polymerase chain reaction. GraphPad 6 Prism v.8 was used to perform the statistical analyses. Comparisons between groups in internal samples were conducted by paired student's t-test, while Mann-Whitney U test was used to analyze TCGA-LUNG data (P < 0.05).Results: Our results indicated miR-30b hypermethylation, miR-30b downregulation and lncRNA MALAT-1 overexpression in NSCLC tumor samples compared with marginal normal samples. These changes were significantly associated with the stage of malignancy like lymph node metastasis. Also, using receiver operating characteristic curve analysis, MALAT-1 expression, and miR-30b methylation and expression patterns were found as possible diagnostic biomarkers for NSCLC (area under the curve was 0.70, 0.67, and 0.74, respectively).Conclusion: We found involvement of miR-30b hypermethylation and downregulation as well as lncRNA MALAT-1 overexpression with tumor outcomes of NSCLC patients.https://mejc.sums.ac.ir/article_50236_38dc29b1d2af2e7f9a0f8e94d34c4c9a.pdfmir-30blncrna malt-1dna methylationcarcinoma, non-small-cell lung, neoplasms |
| spellingShingle | Marziyeh Memarzadeh Habib Zarredar Milad Asadi Armin Sadeghi Venus Zafari Shahryar Hashemzadeh Hamed Sabbagh-Jadid Mortaza Raeisi Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer Middle East Journal of Cancer mir-30b lncrna malt-1 dna methylation carcinoma, non-small-cell lung, neoplasms |
| title | Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer |
| title_full | Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer |
| title_fullStr | Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer |
| title_full_unstemmed | Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer |
| title_short | Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer |
| title_sort | exploring mir 30b methylation and malat 1 expression as diagnostic biomarkers for non small cell lung cancer |
| topic | mir-30b lncrna malt-1 dna methylation carcinoma, non-small-cell lung, neoplasms |
| url | https://mejc.sums.ac.ir/article_50236_38dc29b1d2af2e7f9a0f8e94d34c4c9a.pdf |
| work_keys_str_mv | AT marziyehmemarzadeh exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT habibzarredar exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT miladasadi exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT arminsadeghi exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT venuszafari exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT shahryarhashemzadeh exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT hamedsabbaghjadid exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer AT mortazaraeisi exploringmir30bmethylationandmalat1expressionasdiagnosticbiomarkersfornonsmallcelllungcancer |