Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli
Calcium-dependent protein kinase (CDPK) is an important mediator for Ca2 + signal recognition and transduction, playing a crucial role in plant stress response. Previous studies have shown that PcCDPK5 may be involved in the response of patchouli to p-hydroxybenzoic acid (p-HBA) stress. In this stud...
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001435 |
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| author | Xiaofeng Liu Jingru Zhou Muhammad Zeeshan Ul Haq Zhineng Fu Guangtao Gu Jing Yu Ya Liu Dongmei Yang Huageng Yang Yougen Wu |
| author_facet | Xiaofeng Liu Jingru Zhou Muhammad Zeeshan Ul Haq Zhineng Fu Guangtao Gu Jing Yu Ya Liu Dongmei Yang Huageng Yang Yougen Wu |
| author_sort | Xiaofeng Liu |
| collection | DOAJ |
| description | Calcium-dependent protein kinase (CDPK) is an important mediator for Ca2 + signal recognition and transduction, playing a crucial role in plant stress response. Previous studies have shown that PcCDPK5 may be involved in the response of patchouli to p-hydroxybenzoic acid (p-HBA) stress. In this study, we further found that the subcellular localization of PcCDPK5 protein is in the cytoplasm, and its gene expression is closely related to continuous cropping (CC) and p-HBA stress. Under p-HBA stress, silencing the PcCDPK5 homologous gene in Nicotiana tabacum leads to decreased antioxidant enzyme activity and increased malondialdehyde (MDA) content, significantly accumulating reactive oxygen species (ROS) and affecting normal plant growth. On the contrary, overexpression of PcCDPK5 can effectively alleviate the damage caused by p-HBA stress to plant bodies. Through this research, the function of PcCDPK5 in response to p-HBA stress has been preliminarily analyzed, laying a theoretical foundation for alleviating CC obstacles in patchouli. |
| format | Article |
| id | doaj-art-1a0dcfa24e1c4de2ac392cbea19d50f2 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-1a0dcfa24e1c4de2ac392cbea19d50f22025-01-31T05:10:00ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117807Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouliXiaofeng Liu0Jingru Zhou1Muhammad Zeeshan Ul Haq2Zhineng Fu3Guangtao Gu4Jing Yu5Ya Liu6Dongmei Yang7Huageng Yang8Yougen Wu9School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaCorresponding author.; School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaCalcium-dependent protein kinase (CDPK) is an important mediator for Ca2 + signal recognition and transduction, playing a crucial role in plant stress response. Previous studies have shown that PcCDPK5 may be involved in the response of patchouli to p-hydroxybenzoic acid (p-HBA) stress. In this study, we further found that the subcellular localization of PcCDPK5 protein is in the cytoplasm, and its gene expression is closely related to continuous cropping (CC) and p-HBA stress. Under p-HBA stress, silencing the PcCDPK5 homologous gene in Nicotiana tabacum leads to decreased antioxidant enzyme activity and increased malondialdehyde (MDA) content, significantly accumulating reactive oxygen species (ROS) and affecting normal plant growth. On the contrary, overexpression of PcCDPK5 can effectively alleviate the damage caused by p-HBA stress to plant bodies. Through this research, the function of PcCDPK5 in response to p-HBA stress has been preliminarily analyzed, laying a theoretical foundation for alleviating CC obstacles in patchouli.http://www.sciencedirect.com/science/article/pii/S0147651325001435Pogostemon cablinContinuous cropping obstacles (CCO)Virus-induced gene silencing (VIGS)Overexpression |
| spellingShingle | Xiaofeng Liu Jingru Zhou Muhammad Zeeshan Ul Haq Zhineng Fu Guangtao Gu Jing Yu Ya Liu Dongmei Yang Huageng Yang Yougen Wu Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli Ecotoxicology and Environmental Safety Pogostemon cablin Continuous cropping obstacles (CCO) Virus-induced gene silencing (VIGS) Overexpression |
| title | Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli |
| title_full | Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli |
| title_fullStr | Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli |
| title_full_unstemmed | Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli |
| title_short | Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli |
| title_sort | functional analysis of the pccdpk5 gene in response to allelopathic substances on p hydroxybenzoic acid p hba stress in patchouli |
| topic | Pogostemon cablin Continuous cropping obstacles (CCO) Virus-induced gene silencing (VIGS) Overexpression |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001435 |
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