Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003
Genetic transformation has been achieved by incubating protoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth w...
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| Main Authors: | CHEN Zhen-ming, GUO Ze-jian, LIN Fu-cheng, LI De-bao |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2001-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.01.0019 |
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