Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003
Genetic transformation has been achieved by incubating protoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth w...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2001-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.01.0019 |
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| author | CHEN Zhen-ming GUO Ze-jian LIN Fu-cheng LI De-bao |
| author_facet | CHEN Zhen-ming GUO Ze-jian LIN Fu-cheng LI De-bao |
| author_sort | CHEN Zhen-ming |
| collection | DOAJ |
| description | Genetic transformation has been achieved by incubating protoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth was inhibited completely when the medium contained 150 μg/mL hygromycin B. Integration of hph gene was verified by PCR and PCR product hybridization analysis. There was no evidence of mitotic of hygromycin B resistance in the transformants. |
| format | Article |
| id | doaj-art-1a088aaf192a48eeadc79c584d9b4b35 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-1a088aaf192a48eeadc79c584d9b4b352025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552001-01-0127192210.3785/1008-9209.2001.01.001910089209Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003CHEN Zhen-mingGUO Ze-jianLIN Fu-chengLI De-baoGenetic transformation has been achieved by incubating protoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth was inhibited completely when the medium contained 150 μg/mL hygromycin B. Integration of hph gene was verified by PCR and PCR product hybridization analysis. There was no evidence of mitotic of hygromycin B resistance in the transformants.https://www.academax.com/doi/10.3785/1008-9209.2001.01.0019Chaetomium globosumtransformationhygromycin B resistance |
| spellingShingle | CHEN Zhen-ming GUO Ze-jian LIN Fu-cheng LI De-bao Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 浙江大学学报. 农业与生命科学版 Chaetomium globosum transformation hygromycin B resistance |
| title | Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 |
| title_full | Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 |
| title_fullStr | Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 |
| title_full_unstemmed | Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 |
| title_short | Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003 |
| title_sort | transformation of chaetomium globosum with hygromycin b resistance plasmids pcb1003 |
| topic | Chaetomium globosum transformation hygromycin B resistance |
| url | https://www.academax.com/doi/10.3785/1008-9209.2001.01.0019 |
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