Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques
Fumagillin is a complex biomolecule. Nowadays, the separation and purification techniques of fumagillin still need to be optimized. In this study, the yield of fumagillin was examined in relation to temperature, pH, inoculation volume, rotation speed, and liquid volume. By using single-factor optimi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/11/588 |
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| author | Zili Feng Pengfei Du Yunzhi Sheng Bin Wang Wang Chen Hao Peng |
| author_facet | Zili Feng Pengfei Du Yunzhi Sheng Bin Wang Wang Chen Hao Peng |
| author_sort | Zili Feng |
| collection | DOAJ |
| description | Fumagillin is a complex biomolecule. Nowadays, the separation and purification techniques of fumagillin still need to be optimized. In this study, the yield of fumagillin was examined in relation to temperature, pH, inoculation volume, rotation speed, and liquid volume. By using single-factor optimization, a factorial design, and response surface methodology, the fumagillin yield increased from 10~15% to 30~35%. After scaling up the fermentation process, the fumagillin yield remained stable at 30–35%. In addition, fumagillin was prepared via high-performance preparative liquid chromatography; the yield of the preparation was 77.29%, and the purity was greater than 99%. To sum up, these findings demonstrated that the issue of low fumagillin yield can be resolved by optimizing the fermentation process and introducing the high-performance liquid chromatographic method for the rapid preparation of fumagillin with high recoveries. The findings of this study may serve as a foundation for the large-scale production of fumagillin. |
| format | Article |
| id | doaj-art-19f04ed78fc342c7aa2c50e892455c42 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-19f04ed78fc342c7aa2c50e892455c422025-08-20T01:53:41ZengMDPI AGFermentation2311-56372024-11-01101158810.3390/fermentation10110588Enhanced Fumagillin Production by Optimizing Fermentation and Purification TechniquesZili Feng0Pengfei Du1Yunzhi Sheng2Bin Wang3Wang Chen4Hao Peng5College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaFumagillin is a complex biomolecule. Nowadays, the separation and purification techniques of fumagillin still need to be optimized. In this study, the yield of fumagillin was examined in relation to temperature, pH, inoculation volume, rotation speed, and liquid volume. By using single-factor optimization, a factorial design, and response surface methodology, the fumagillin yield increased from 10~15% to 30~35%. After scaling up the fermentation process, the fumagillin yield remained stable at 30–35%. In addition, fumagillin was prepared via high-performance preparative liquid chromatography; the yield of the preparation was 77.29%, and the purity was greater than 99%. To sum up, these findings demonstrated that the issue of low fumagillin yield can be resolved by optimizing the fermentation process and introducing the high-performance liquid chromatographic method for the rapid preparation of fumagillin with high recoveries. The findings of this study may serve as a foundation for the large-scale production of fumagillin.https://www.mdpi.com/2311-5637/10/11/588fumagillinfermentation process optimizationscaling fermentationoptimization of the extraction processpreparation chromatography purification |
| spellingShingle | Zili Feng Pengfei Du Yunzhi Sheng Bin Wang Wang Chen Hao Peng Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques Fermentation fumagillin fermentation process optimization scaling fermentation optimization of the extraction process preparation chromatography purification |
| title | Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques |
| title_full | Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques |
| title_fullStr | Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques |
| title_full_unstemmed | Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques |
| title_short | Enhanced Fumagillin Production by Optimizing Fermentation and Purification Techniques |
| title_sort | enhanced fumagillin production by optimizing fermentation and purification techniques |
| topic | fumagillin fermentation process optimization scaling fermentation optimization of the extraction process preparation chromatography purification |
| url | https://www.mdpi.com/2311-5637/10/11/588 |
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