Anterior chamber depth in mice is controlled by several quantitative trait loci.
Anterior chamber depth (ACD) is a quantitative trait associated with primary angle closure glaucoma (PACG). Although ACD is highly heritable, known genetic variations explain a small fraction of the phenotypic variability. The purpose of this study was to identify additional ACD-influencing loci usi...
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0286897 |
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| author | Demelza R Larson Allysa J Kimber Kacie J Meyer Michael G Anderson |
| author_facet | Demelza R Larson Allysa J Kimber Kacie J Meyer Michael G Anderson |
| author_sort | Demelza R Larson |
| collection | DOAJ |
| description | Anterior chamber depth (ACD) is a quantitative trait associated with primary angle closure glaucoma (PACG). Although ACD is highly heritable, known genetic variations explain a small fraction of the phenotypic variability. The purpose of this study was to identify additional ACD-influencing loci using strains of mice. Cohorts of 86 N2 and 111 F2 mice were generated from crosses between recombinant inbred BXD24/TyJ and wild-derived CAST/EiJ mice. Using anterior chamber optical coherence tomography, mice were phenotyped at 10-12 weeks of age, genotyped based on 93 genome-wide SNPs, and subjected to quantitative trait locus (QTL) analysis. In an analysis of ACD among all mice, six loci passed the significance threshold of p = 0.05 and persisted after multiple regression analysis. These were on chromosomes 6, 7, 11, 12, 15 and 17 (named Acdq6, Acdq7, Acdq11, Acdq12, Acdq15, and Acdq17, respectively). Our findings demonstrate a quantitative multi-genic pattern of ACD inheritance in mice and identify six previously unrecognized ACD-influencing loci. We have taken a unique approach to studying the anterior chamber depth phenotype by using mice as genetic tool to examine this continuously distributed trait. |
| format | Article |
| id | doaj-art-19ece14a7dfc440f81f862df0bcec414 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-19ece14a7dfc440f81f862df0bcec4142025-08-20T03:42:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01188e028689710.1371/journal.pone.0286897Anterior chamber depth in mice is controlled by several quantitative trait loci.Demelza R LarsonAllysa J KimberKacie J MeyerMichael G AndersonAnterior chamber depth (ACD) is a quantitative trait associated with primary angle closure glaucoma (PACG). Although ACD is highly heritable, known genetic variations explain a small fraction of the phenotypic variability. The purpose of this study was to identify additional ACD-influencing loci using strains of mice. Cohorts of 86 N2 and 111 F2 mice were generated from crosses between recombinant inbred BXD24/TyJ and wild-derived CAST/EiJ mice. Using anterior chamber optical coherence tomography, mice were phenotyped at 10-12 weeks of age, genotyped based on 93 genome-wide SNPs, and subjected to quantitative trait locus (QTL) analysis. In an analysis of ACD among all mice, six loci passed the significance threshold of p = 0.05 and persisted after multiple regression analysis. These were on chromosomes 6, 7, 11, 12, 15 and 17 (named Acdq6, Acdq7, Acdq11, Acdq12, Acdq15, and Acdq17, respectively). Our findings demonstrate a quantitative multi-genic pattern of ACD inheritance in mice and identify six previously unrecognized ACD-influencing loci. We have taken a unique approach to studying the anterior chamber depth phenotype by using mice as genetic tool to examine this continuously distributed trait.https://doi.org/10.1371/journal.pone.0286897 |
| spellingShingle | Demelza R Larson Allysa J Kimber Kacie J Meyer Michael G Anderson Anterior chamber depth in mice is controlled by several quantitative trait loci. PLoS ONE |
| title | Anterior chamber depth in mice is controlled by several quantitative trait loci. |
| title_full | Anterior chamber depth in mice is controlled by several quantitative trait loci. |
| title_fullStr | Anterior chamber depth in mice is controlled by several quantitative trait loci. |
| title_full_unstemmed | Anterior chamber depth in mice is controlled by several quantitative trait loci. |
| title_short | Anterior chamber depth in mice is controlled by several quantitative trait loci. |
| title_sort | anterior chamber depth in mice is controlled by several quantitative trait loci |
| url | https://doi.org/10.1371/journal.pone.0286897 |
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