Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA
The binding of TiCl2(dpme)2 (1), (dpme = 6,6′-dimethyl-2,2′-bipyridine), Ti(ada)2(bzac)2 (2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4′-dimethyl-2,2′-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, a...
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Wiley
2015-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2015/836928 |
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| author | Pamita Awasthi Nitesh Kumar Raj Kaushal Mohan Kumar Shrikant Kukreti |
| author_facet | Pamita Awasthi Nitesh Kumar Raj Kaushal Mohan Kumar Shrikant Kukreti |
| author_sort | Pamita Awasthi |
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| description | The binding of TiCl2(dpme)2 (1), (dpme = 6,6′-dimethyl-2,2′-bipyridine), Ti(ada)2(bzac)2 (2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4′-dimethyl-2,2′-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, and circular dichroism spectroscopy. In UV-visible study complexes 1, 2, and 3 showed red, blue, and red shifts, respectively, upon the addition of ct-DNA along with a significant hyperchromism. The intrinsic binding constants (Kb) calculated from UV-visible absorption studies were 2.3 × 103 M−1, 3.3 × 103 M−1 and, 7.1 × 103 M−1 for complexes 1, 2, and 3, respectively. The change in melting temperature (ΔTm) was calculated to be 2-3°C for each complex. Circular dichroism (CD) study showed blue shift for complex 2 and red shift for complexes 1 and 3 along with rise in molecular ellipticity upon the addition of complexes. Results suggest a binding mode of complex 2 different than 1 and 3. |
| format | Article |
| id | doaj-art-19e30e3d8ed341c48660156f7667bd33 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | Biochemistry Research International |
| spelling | doaj-art-19e30e3d8ed341c48660156f7667bd332025-02-03T01:25:05ZengWileyBiochemistry Research International2090-22472090-22552015-01-01201510.1155/2015/836928836928Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNAPamita Awasthi0Nitesh Kumar1Raj Kaushal2Mohan Kumar3Shrikant Kukreti4Department of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, IndiaDepartment of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, IndiaDepartment of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, IndiaDepartment of Chemistry North Campus, University of Delhi, Delhi 110007, IndiaDepartment of Chemistry North Campus, University of Delhi, Delhi 110007, IndiaThe binding of TiCl2(dpme)2 (1), (dpme = 6,6′-dimethyl-2,2′-bipyridine), Ti(ada)2(bzac)2 (2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4′-dimethyl-2,2′-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, and circular dichroism spectroscopy. In UV-visible study complexes 1, 2, and 3 showed red, blue, and red shifts, respectively, upon the addition of ct-DNA along with a significant hyperchromism. The intrinsic binding constants (Kb) calculated from UV-visible absorption studies were 2.3 × 103 M−1, 3.3 × 103 M−1 and, 7.1 × 103 M−1 for complexes 1, 2, and 3, respectively. The change in melting temperature (ΔTm) was calculated to be 2-3°C for each complex. Circular dichroism (CD) study showed blue shift for complex 2 and red shift for complexes 1 and 3 along with rise in molecular ellipticity upon the addition of complexes. Results suggest a binding mode of complex 2 different than 1 and 3.http://dx.doi.org/10.1155/2015/836928 |
| spellingShingle | Pamita Awasthi Nitesh Kumar Raj Kaushal Mohan Kumar Shrikant Kukreti Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA Biochemistry Research International |
| title | Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA |
| title_full | Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA |
| title_fullStr | Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA |
| title_full_unstemmed | Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA |
| title_short | Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA |
| title_sort | comparative in vitro binding studies of ticl2 dpme 2 ti ada 2 bzac 2 and ticl2 bzac bpme titanium complexes with calf thymus dna |
| url | http://dx.doi.org/10.1155/2015/836928 |
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