Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells
We hypothesize that the anti - apoptotic response in mesenchymal stem cells (MSC) under low doses of radiation may develop through the participation of an extracellular mediator of signal transmission between cells - fragments of oxidized cell - free DNA (cfDNA).Aim. To investigate the effect of radi...
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| Format: | Article |
| Language: | Russian |
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Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2019-09-01
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| Series: | Сеченовский вестник |
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| Online Access: | https://www.sechenovmedj.com/jour/article/view/107 |
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| author | A. A. Kaliyanov M. S. Konkova L. V. Kameneva E. M. Malinovskaya M. S. Abramova O. A. Dolgikh E. S. Ershova N. N. Veiko N. K. Golubeva P. E. Umriukhin S. V. Kostyuk |
| author_facet | A. A. Kaliyanov M. S. Konkova L. V. Kameneva E. M. Malinovskaya M. S. Abramova O. A. Dolgikh E. S. Ershova N. N. Veiko N. K. Golubeva P. E. Umriukhin S. V. Kostyuk |
| author_sort | A. A. Kaliyanov |
| collection | DOAJ |
| description | We hypothesize that the anti - apoptotic response in mesenchymal stem cells (MSC) under low doses of radiation may develop through the participation of an extracellular mediator of signal transmission between cells - fragments of oxidized cell - free DNA (cfDNA).Aim. To investigate the effect of radiation in low doses (10 sGy) and oxidized fragments of cfDNA on the activation of genes in MSCs signalling cascades that regulate the cellular response to DNA damage. Materials and methods. We investigated the effect of radiation in low doses (10 cGy) and fragments of oxidized cfDNA (concentration 50 ng/ml) on the activation of signalling gene cascades in MSC that regulate the cellular response to DNA damage.Results. We have shown, that in MSC under small doses of radiation for 15-40 minutes the transcriptional activity of pro - apoptotic genes is activated, the level of apoptosis increases and due to the death of part of the cells in the culture of MSC, fragments of oxidized cfDNA are formed. 3-72 hours later the expression of genes ING2, HUWE1, TP53, BBC3, NOXA, P53AIP1, BAX, BAK1, MMP7, ENDOG decreases 1.5-3 times ( p |
| format | Article |
| id | doaj-art-19e0a3124f834b9e82aafed4785f6384 |
| institution | DOAJ |
| issn | 2218-7332 2658-3348 |
| language | Russian |
| publishDate | 2019-09-01 |
| publisher | Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) |
| record_format | Article |
| series | Сеченовский вестник |
| spelling | doaj-art-19e0a3124f834b9e82aafed4785f63842025-08-20T02:53:22ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482019-09-0110341210.47093/22187332.2019.3.4-12106Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cellsA. A. Kaliyanov0M. S. Konkova1L. V. Kameneva2E. M. Malinovskaya3M. S. Abramova4O. A. Dolgikh5E. S. Ershova6N. N. Veiko7N. K. Golubeva8P. E. Umriukhin9S. V. Kostyuk10Research Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical GeneticsResearch Centre for Medical Genetics; Sechenov First Moscow State Medical University (Sechenov University); Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Centre of Intensive Care Medicine and RehabilitologyResearch Centre for Medical GeneticsSechenov First Moscow State Medical University (Sechenov University)Research Centre for Medical Genetics; Sechenov First Moscow State Medical University (Sechenov University); Anokhin Institute of Normal PhysiologyResearch Centre for Medical Genetics; Sechenov First Moscow State Medical University (Sechenov University); Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Centre of Intensive Care Medicine and RehabilitologyWe hypothesize that the anti - apoptotic response in mesenchymal stem cells (MSC) under low doses of radiation may develop through the participation of an extracellular mediator of signal transmission between cells - fragments of oxidized cell - free DNA (cfDNA).Aim. To investigate the effect of radiation in low doses (10 sGy) and oxidized fragments of cfDNA on the activation of genes in MSCs signalling cascades that regulate the cellular response to DNA damage. Materials and methods. We investigated the effect of radiation in low doses (10 cGy) and fragments of oxidized cfDNA (concentration 50 ng/ml) on the activation of signalling gene cascades in MSC that regulate the cellular response to DNA damage.Results. We have shown, that in MSC under small doses of radiation for 15-40 minutes the transcriptional activity of pro - apoptotic genes is activated, the level of apoptosis increases and due to the death of part of the cells in the culture of MSC, fragments of oxidized cfDNA are formed. 3-72 hours later the expression of genes ING2, HUWE1, TP53, BBC3, NOXA, P53AIP1, BAX, BAK1, MMP7, ENDOG decreases 1.5-3 times ( phttps://www.sechenovmedj.com/jour/article/view/107cell free dnalow doses of ionizing radiationmesenchymal stem cellsapoptosis |
| spellingShingle | A. A. Kaliyanov M. S. Konkova L. V. Kameneva E. M. Malinovskaya M. S. Abramova O. A. Dolgikh E. S. Ershova N. N. Veiko N. K. Golubeva P. E. Umriukhin S. V. Kostyuk Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells Сеченовский вестник cell free dna low doses of ionizing radiation mesenchymal stem cells apoptosis |
| title | Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| title_full | Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| title_fullStr | Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| title_full_unstemmed | Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| title_short | Small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| title_sort | small doses of radiation activate a signaling pathway aimed at apoptosis inhibition in mesenchymal stem cells |
| topic | cell free dna low doses of ionizing radiation mesenchymal stem cells apoptosis |
| url | https://www.sechenovmedj.com/jour/article/view/107 |
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