Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and b...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.736301/full |
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| author | Yi Zhan Bing Deng Huixian Wu Changpeng Xu Ruiying Wang Wenqiang Li Zhixiong Pan Zhixiong Pan |
| author_facet | Yi Zhan Bing Deng Huixian Wu Changpeng Xu Ruiying Wang Wenqiang Li Zhixiong Pan Zhixiong Pan |
| author_sort | Yi Zhan |
| collection | DOAJ |
| description | Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis in vitro. The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, in vivo studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas. |
| format | Article |
| id | doaj-art-19df11f067eb4317af9af98c6097bf4e |
| institution | DOAJ |
| issn | 1663-9812 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-19df11f067eb4317af9af98c6097bf4e2025-08-20T03:20:37ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-11-011210.3389/fphar.2021.736301736301Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and AngiogenesisYi Zhan0Bing Deng1Huixian Wu2Changpeng Xu3Ruiying Wang4Wenqiang Li5Zhixiong Pan6Zhixiong Pan7Department of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, ChinaDepartment of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, ChinaDepartment of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, ChinaDepartment of Orthopaedics, Guangdong Second Provincial General Hospital, Guangzhou, ChinaDepartment of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, ChinaEngineering Technology Research Center for Sports Assistive Devices of Guangdong, Guangzhou Sport University, Guangzhou, ChinaDepartment of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, ChinaGuangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaLiquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis in vitro. The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, in vivo studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas.https://www.frontiersin.org/articles/10.3389/fphar.2021.736301/fullliquid crystal fiberbiomimetic mineralizationosteogenic differentiationvascularizationregeneration |
| spellingShingle | Yi Zhan Bing Deng Huixian Wu Changpeng Xu Ruiying Wang Wenqiang Li Zhixiong Pan Zhixiong Pan Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis Frontiers in Pharmacology liquid crystal fiber biomimetic mineralization osteogenic differentiation vascularization regeneration |
| title | Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis |
| title_full | Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis |
| title_fullStr | Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis |
| title_full_unstemmed | Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis |
| title_short | Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis |
| title_sort | biomineralized composite liquid crystal fiber scaffold promotes bone regeneration by enhancement of osteogenesis and angiogenesis |
| topic | liquid crystal fiber biomimetic mineralization osteogenic differentiation vascularization regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.736301/full |
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