Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques.
In the present study, a 2,4-dichlorophenoxyacetic acid (2,4-D) degrading bacterial strain CY-1 was isolated from the forest soil. Based on physiological, biochemical and 16S rRNA gene sequence analysis it was identified as Cupriavidus sp. CY-1. Further 2,4-D degradation experiments at different conc...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145057&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722980998840320 |
|---|---|
| author | Young-Cheol Chang M Venkateswar Reddy Honoka Umemoto Yuki Sato Mi-Hye Kang Yuka Yajima Shintaro Kikuchi |
| author_facet | Young-Cheol Chang M Venkateswar Reddy Honoka Umemoto Yuki Sato Mi-Hye Kang Yuka Yajima Shintaro Kikuchi |
| author_sort | Young-Cheol Chang |
| collection | DOAJ |
| description | In the present study, a 2,4-dichlorophenoxyacetic acid (2,4-D) degrading bacterial strain CY-1 was isolated from the forest soil. Based on physiological, biochemical and 16S rRNA gene sequence analysis it was identified as Cupriavidus sp. CY-1. Further 2,4-D degradation experiments at different concentrations (200 to 800 mg l(-1)) were carried out using CY-1. Effect of NaCl and KNO3 on 2,4-D degradation was also evaluated. Degradation of 2,4-D and the metabolites produced during degradation process were analyzed using high pressure liquid chromatography (HPLC) and GC-MS respectively. The amount of chloride ions produced during the 2,4-D degradation were analyzed by Ion chromatography (IC) and it is stoichiometric with 2,4-D dechlorination. Furthermore two different types of soils collected from two different sources were used for 2,4-D degradation studies. The isolated strain CY-1 was bio-augmented into 2,4-D contaminated soils to analyze its degradation ability. Culture independent methods like denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP), and culture dependent methods like colony forming units (CFU) and most probable number (MPN) were used to analyze the survivability of strain CY-1 in contaminated soil. Results of T-RFLP were coincident with the DGGE analysis. From the DGGE, T-RFLP, MPN and HPLC results it was concluded that strain CY-1 effectively degraded 2,4-D without disturbing the ecosystem of soil indigenous microorganisms. |
| format | Article |
| id | doaj-art-19d8ec0a94d04c8d8c4764cbc146bd9c |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-19d8ec0a94d04c8d8c4764cbc146bd9c2025-08-20T03:11:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014505710.1371/journal.pone.0145057Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques.Young-Cheol ChangM Venkateswar ReddyHonoka UmemotoYuki SatoMi-Hye KangYuka YajimaShintaro KikuchiIn the present study, a 2,4-dichlorophenoxyacetic acid (2,4-D) degrading bacterial strain CY-1 was isolated from the forest soil. Based on physiological, biochemical and 16S rRNA gene sequence analysis it was identified as Cupriavidus sp. CY-1. Further 2,4-D degradation experiments at different concentrations (200 to 800 mg l(-1)) were carried out using CY-1. Effect of NaCl and KNO3 on 2,4-D degradation was also evaluated. Degradation of 2,4-D and the metabolites produced during degradation process were analyzed using high pressure liquid chromatography (HPLC) and GC-MS respectively. The amount of chloride ions produced during the 2,4-D degradation were analyzed by Ion chromatography (IC) and it is stoichiometric with 2,4-D dechlorination. Furthermore two different types of soils collected from two different sources were used for 2,4-D degradation studies. The isolated strain CY-1 was bio-augmented into 2,4-D contaminated soils to analyze its degradation ability. Culture independent methods like denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP), and culture dependent methods like colony forming units (CFU) and most probable number (MPN) were used to analyze the survivability of strain CY-1 in contaminated soil. Results of T-RFLP were coincident with the DGGE analysis. From the DGGE, T-RFLP, MPN and HPLC results it was concluded that strain CY-1 effectively degraded 2,4-D without disturbing the ecosystem of soil indigenous microorganisms.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145057&type=printable |
| spellingShingle | Young-Cheol Chang M Venkateswar Reddy Honoka Umemoto Yuki Sato Mi-Hye Kang Yuka Yajima Shintaro Kikuchi Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. PLoS ONE |
| title | Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. |
| title_full | Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. |
| title_fullStr | Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. |
| title_full_unstemmed | Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. |
| title_short | Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques. |
| title_sort | bio augmentation of cupriavidus sp cy 1 into 2 4 d contaminated soil microbial community analysis by culture dependent and independent techniques |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145057&type=printable |
| work_keys_str_mv | AT youngcheolchang bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT mvenkateswarreddy bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT honokaumemoto bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT yukisato bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT mihyekang bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT yukayajima bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques AT shintarokikuchi bioaugmentationofcupriavidusspcy1into24dcontaminatedsoilmicrobialcommunityanalysisbyculturedependentandindependenttechniques |