How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones
Elevated concentrations of pharmaceutically active compounds (PhACs) in the water bodies are posing a serious threat to the aquatic microbiota and other organisms. In this context, anaerobic ammonium oxidizing (anammox) bacteria carry a great potential to degrade PhACs through their innate metabolic...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024173707 |
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| author | Faysal-Al Mamun Rohit Kumar Kelvin Ugochukwu Anwuta Sovik Das Madis Jaagura Koit Herodes Tetyana Kyrpel Agnieszka Fiszka Borzyszkowska Anna Zielińska-Jurek Zane Vincevica-Gaile Juris Burlakovs Andrey E. Krauklis Mohamad Nor Azra Md Salauddin Jiexi Zhong Taavo Tenno Kai Bester Ivar Zekker |
| author_facet | Faysal-Al Mamun Rohit Kumar Kelvin Ugochukwu Anwuta Sovik Das Madis Jaagura Koit Herodes Tetyana Kyrpel Agnieszka Fiszka Borzyszkowska Anna Zielińska-Jurek Zane Vincevica-Gaile Juris Burlakovs Andrey E. Krauklis Mohamad Nor Azra Md Salauddin Jiexi Zhong Taavo Tenno Kai Bester Ivar Zekker |
| author_sort | Faysal-Al Mamun |
| collection | DOAJ |
| description | Elevated concentrations of pharmaceutically active compounds (PhACs) in the water bodies are posing a serious threat to the aquatic microbiota and other organisms. In this context, anaerobic ammonium oxidizing (anammox) bacteria carry a great potential to degrade PhACs through their innate metabolic pathways. This study investigates the influence of short-term exposure to lower and higher concentrations (0.8 mg L−1, 0.06 mg L−1, respectively) of antibiotics on the anammox process under distinct operational conditions (starvation/non-starvation) in moving bed biofilm reactor (MBBR). During batch operations that lasted for up to 6 h, the total nitrogen removal efficiency (TNRE) and total nitrogen conversion rate (TNCR) reached a maximum of 93 ± 5 % and 6.97 ± 1.30 mg N g−1 TSS d−1, respectively. Evidently, at higher PhAC levels, the anammox process was active, and up to 75 % PhAC removal efficiency was obtained within 6 h of the batch cycle. Most importantly, the anammox biofilm effectively eliminated the PhACs compounds, i.e., ciprofloxacin (CIP), ofloxacin (OFL), and norfloxacin (NOR) present at higher (0.8 mg L−1) and lower (0.06 mg L−1) total PhACs (sum of CIP, NOR, OFL) concentrations. Furthermore, 16S rRNA sequencing analyses showed a mixture of nitrifying, denitrifying, and anammox bacterial commodities enriched on the carriers' surface with a high relative abundance of Candidatus Brocadia, primarily responsible for catalyzing the anammox process. This study showed the intricate relationship between PhAC concentrations, TNCR, and antibiotic elimination in the wastewater treatment, and the results obtained set up a new breakthrough in wastewater treatment. Future research should investigate the mechanisms that underlie the anammox biofilms' resistance to various types of PhACs and investigate the long-term stability and scalability of this process with real wastewater influents. |
| format | Article |
| id | doaj-art-19c22f62f254402590066146dafba65e |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-19c22f62f254402590066146dafba65e2025-01-17T04:50:58ZengElsevierHeliyon2405-84402025-01-01111e41339How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolonesFaysal-Al Mamun0Rohit Kumar1Kelvin Ugochukwu Anwuta2Sovik Das3Madis Jaagura4Koit Herodes5Tetyana Kyrpel6Agnieszka Fiszka Borzyszkowska7Anna Zielińska-Jurek8Zane Vincevica-Gaile9Juris Burlakovs10Andrey E. Krauklis11Mohamad Nor Azra12Md Salauddin13Jiexi Zhong14Taavo Tenno15Kai Bester16Ivar Zekker17Institute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaInstitute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaInstitute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaIndian Institute of Technology Delhi, Hauz Khas, New Delhi, 110 016, IndiaInstitute of Genomics, University of Tartu, Riia 23b, Tartu, 51010, EstoniaInstitute of Chemistry, Chair of Analytics, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaInstitute of Chemistry, Chair of Analytics, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaDepartment of Environmental Toxicology, Faculty of Health Sciences, Medical University of Gdansk, Dębowa Str. 23A, 80-204, Gdansk, PolandDepartment of Process Engineering and Chemical Technology, Gdansk University of Technology, Narutowicza 11/12, 80-233, Gdansk, PolandDepartment of Environmental Science, University of Latvia, Jelgavas Street 1, LV-1004, Riga, LatviaFaculty of Civil and Mechanical Engineering, Riga Technical University, LV-1048, Riga, LatviaASEMlab – Laboratory of Advanced and Sustainable Engineering Materials, Department of Manufacturing and Civil Engineering, NTNU – Norwegian University of Science and Technology, 2815, Gjøvik, NorwayInstitute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaUCD Dooge Centre for Water Resources Research, School of Civil Engineering, University College Dublin, IrelandDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, DenmarkInstitute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, EstoniaDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark; WATEC – Centre for Water Technology, Aarhus University, Ny Munkegade 120, Aarhus, 8000, Denmark; Corresponding author. Institute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, Estonia.Institute of Chemistry, University of Tartu, 14a Ravila St., 50411, Tartu, Estonia; Corresponding author.Elevated concentrations of pharmaceutically active compounds (PhACs) in the water bodies are posing a serious threat to the aquatic microbiota and other organisms. In this context, anaerobic ammonium oxidizing (anammox) bacteria carry a great potential to degrade PhACs through their innate metabolic pathways. This study investigates the influence of short-term exposure to lower and higher concentrations (0.8 mg L−1, 0.06 mg L−1, respectively) of antibiotics on the anammox process under distinct operational conditions (starvation/non-starvation) in moving bed biofilm reactor (MBBR). During batch operations that lasted for up to 6 h, the total nitrogen removal efficiency (TNRE) and total nitrogen conversion rate (TNCR) reached a maximum of 93 ± 5 % and 6.97 ± 1.30 mg N g−1 TSS d−1, respectively. Evidently, at higher PhAC levels, the anammox process was active, and up to 75 % PhAC removal efficiency was obtained within 6 h of the batch cycle. Most importantly, the anammox biofilm effectively eliminated the PhACs compounds, i.e., ciprofloxacin (CIP), ofloxacin (OFL), and norfloxacin (NOR) present at higher (0.8 mg L−1) and lower (0.06 mg L−1) total PhACs (sum of CIP, NOR, OFL) concentrations. Furthermore, 16S rRNA sequencing analyses showed a mixture of nitrifying, denitrifying, and anammox bacterial commodities enriched on the carriers' surface with a high relative abundance of Candidatus Brocadia, primarily responsible for catalyzing the anammox process. This study showed the intricate relationship between PhAC concentrations, TNCR, and antibiotic elimination in the wastewater treatment, and the results obtained set up a new breakthrough in wastewater treatment. Future research should investigate the mechanisms that underlie the anammox biofilms' resistance to various types of PhACs and investigate the long-term stability and scalability of this process with real wastewater influents.http://www.sciencedirect.com/science/article/pii/S2405844024173707Nitrogen removalMoving bed biofilm reactorAnammoxPharmaceutically active compoundsAdsorption |
| spellingShingle | Faysal-Al Mamun Rohit Kumar Kelvin Ugochukwu Anwuta Sovik Das Madis Jaagura Koit Herodes Tetyana Kyrpel Agnieszka Fiszka Borzyszkowska Anna Zielińska-Jurek Zane Vincevica-Gaile Juris Burlakovs Andrey E. Krauklis Mohamad Nor Azra Md Salauddin Jiexi Zhong Taavo Tenno Kai Bester Ivar Zekker How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones Heliyon Nitrogen removal Moving bed biofilm reactor Anammox Pharmaceutically active compounds Adsorption |
| title | How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones |
| title_full | How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones |
| title_fullStr | How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones |
| title_full_unstemmed | How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones |
| title_short | How resistant is anammox biofilm against antibiotics: A special insight into anammox response towards fluoroquinolones |
| title_sort | how resistant is anammox biofilm against antibiotics a special insight into anammox response towards fluoroquinolones |
| topic | Nitrogen removal Moving bed biofilm reactor Anammox Pharmaceutically active compounds Adsorption |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024173707 |
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