Possibilities of MMSC secretome application for osteorepair stimulation: an experimental study
Abstract. The article presents the results of the experimental study of osteoreparation under the action of multipotent mesenchymal stromal cells (MMSC) secretome. White mongrel rats, distributed into control (natural healing) and experimental (with application of MMSCs secretome) groups with applic...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
LLC "Agency of Creative Technologies"
2025-02-01
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| Series: | Ветеринария и кормление |
| Subjects: | |
| Online Access: | https://vetkorm.ru/en/doi/2025-1-10-30917-att-vk-1814-9588-2025-1-10-possibilities-of-mmsc-secretome-application-for-osteorepair-stimulation-an-experimental-study-kuznetsova-m-a-borhunova-e-n-stepanishin-v-v-pozyabin-s-v-2/ |
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| Summary: | Abstract. The article presents the results of the experimental study of osteoreparation under the action of multipotent mesenchymal stromal cells (MMSC) secretome. White mongrel rats, distributed into control (natural healing) and experimental (with application of MMSCs secretome) groups with application of a defect in the diaphysis region of the femur were studied in the experiment. The animals were taken out of the experiment by anesthesia overdosing on the 7th, 14th and 90th days. On the basis of light and scanning electron microscopy methods it was shown that in the experimental group when secretome was used in comparison with the control by the 7th day there was more active vascularization and more pronounced stimulation of reparative osteogenesis, which was manifested by the formation of a developed network of bone trabeculae and in the connective tissue between the edges of the bone defect. By 14 days, in the experimental group in comparison with the control group there is a better consolidation of bone trabeculae of the regenerate with the defect edges and abundant vascularization, and in 90 days the regenerate has signs of histotypic structure. Morphological data positively correlate with the results of blood biochemical studies and computer tomographic data. MMSCs secretome stimulates reparative osteogenesis and leads to earlier defect closure and more active remodeling of the regenerate, which acquires a structure close to the histotypic structure. The established correlations between the study of histological characteristics of bone regenerate and densitometric indices of bone tissue and can serve as criteria for assessing reparative regeneration in experiment and clinical practice. |
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| ISSN: | 1814-9588 |