Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins
In this retrospective study, it was evaluated the antimicrobial susceptibility profiles of Bacillus anthracis isolates obtained from human, various samples (blood, spleen, lung, liver, meat) of dead animals (cattle, sheep, dog, horse) that died from anthrax and soil samples from the animal burial ar...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2024-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3170 |
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| author | Elif ÇELİK Aliye GÜLMEZ SAĞLAM Fatih BÜYÜK Salih OTLU Mitat ŞAHİN Özgür ÇELEBİ Mustafa Reha COŞKUN Seda GÖKDEMİR Yaren ERSOY Eray BÜYÜK Bengisu ERŞAHİN |
| author_facet | Elif ÇELİK Aliye GÜLMEZ SAĞLAM Fatih BÜYÜK Salih OTLU Mitat ŞAHİN Özgür ÇELEBİ Mustafa Reha COŞKUN Seda GÖKDEMİR Yaren ERSOY Eray BÜYÜK Bengisu ERŞAHİN |
| author_sort | Elif ÇELİK |
| collection | DOAJ |
| description | In this retrospective study, it was evaluated the antimicrobial susceptibility profiles
of Bacillus anthracis isolates obtained from human, various samples (blood, spleen,
lung, liver, meat) of dead animals (cattle, sheep, dog, horse) that died from anthrax
and soil samples from the animal burial areas between 2012 and 2023 from Kars
province of Türkiye. In this context, a total of 87 B. anthracis isolates obtained from 1
human, two horses, 51 cattle, 7 sheep, 1 dog, and 25 soil were investigated. The isolates
were confirmed as B. anthracis using protective antigen (PA) and capsule (Cap) gen
specific PCRs. The Kirby-Bauer disk diffusion method was used for deternination
of antimicrobial susceptibility. Ten antimicrobials including penicillin, amoxicillin,
trimethoprim-sulfometoxazole, erythromycin, meropenem, streptomycin, ofloxacin,
ciprofloxacin, chloramphenicol, and clindamycin were tested. As a result of PCR, all
isolates were confirmed as fully virulent field strains of B. anthracis. All isolates were
found as susceptible to penicillin, amoxicillin, ofloxacin and ciprofloxacin. Since the
last studies in the region, a change in the antimicrobial profile of B. anthracis strains
was observed only for trimethoprim-sulfomethoxazole among the antimicrobials
tested, a transition from susceptibility to resistance. In conclusion, penicillin and
amoxicillin, are still the antibiotic of first choice for the prophylaxis and treatment
of anthrax. Ofloxacin and ciprofloxacin are also effective enough to be prescribed for
treatment. |
| format | Article |
| id | doaj-art-19b295a9eeb8412cbac5c18d11db10aa |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-19b295a9eeb8412cbac5c18d11db10aa2025-01-04T21:15:10ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-12-0131112513210.9775/kvfd.2024.330333170Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different originsElif ÇELİK0Aliye GÜLMEZ SAĞLAM1Fatih BÜYÜK2Salih OTLU3Mitat ŞAHİNÖzgür ÇELEBİ4Mustafa Reha COŞKUN5Seda GÖKDEMİR6Yaren ERSOY7Eray BÜYÜK8Bengisu ERŞAHİN9Kafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Medicine, Basic Medical Sciences, Department of Medical Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36300 Kars - TÜRKİYEKafkas University, Institute of Health Sciences, TR-36300 Kars - TÜRKİYEIn this retrospective study, it was evaluated the antimicrobial susceptibility profiles of Bacillus anthracis isolates obtained from human, various samples (blood, spleen, lung, liver, meat) of dead animals (cattle, sheep, dog, horse) that died from anthrax and soil samples from the animal burial areas between 2012 and 2023 from Kars province of Türkiye. In this context, a total of 87 B. anthracis isolates obtained from 1 human, two horses, 51 cattle, 7 sheep, 1 dog, and 25 soil were investigated. The isolates were confirmed as B. anthracis using protective antigen (PA) and capsule (Cap) gen specific PCRs. The Kirby-Bauer disk diffusion method was used for deternination of antimicrobial susceptibility. Ten antimicrobials including penicillin, amoxicillin, trimethoprim-sulfometoxazole, erythromycin, meropenem, streptomycin, ofloxacin, ciprofloxacin, chloramphenicol, and clindamycin were tested. As a result of PCR, all isolates were confirmed as fully virulent field strains of B. anthracis. All isolates were found as susceptible to penicillin, amoxicillin, ofloxacin and ciprofloxacin. Since the last studies in the region, a change in the antimicrobial profile of B. anthracis strains was observed only for trimethoprim-sulfomethoxazole among the antimicrobials tested, a transition from susceptibility to resistance. In conclusion, penicillin and amoxicillin, are still the antibiotic of first choice for the prophylaxis and treatment of anthrax. Ofloxacin and ciprofloxacin are also effective enough to be prescribed for treatment.https://vetdergikafkas.org/pdf.php?id=3170animalantimicrobial susceptibilitybacillus anthracishumanpcrsoil |
| spellingShingle | Elif ÇELİK Aliye GÜLMEZ SAĞLAM Fatih BÜYÜK Salih OTLU Mitat ŞAHİN Özgür ÇELEBİ Mustafa Reha COŞKUN Seda GÖKDEMİR Yaren ERSOY Eray BÜYÜK Bengisu ERŞAHİN Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins Kafkas Universitesi Veteriner Fakültesi Dergisi animal antimicrobial susceptibility bacillus anthracis human pcr soil |
| title | Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| title_full | Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| title_fullStr | Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| title_full_unstemmed | Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| title_short | Antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| title_sort | antimicrobial susceptibility patterns of bacillus anthracis isolates obtained from different origins |
| topic | animal antimicrobial susceptibility bacillus anthracis human pcr soil |
| url | https://vetdergikafkas.org/pdf.php?id=3170 |
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