Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury
Abstract Repeated closed-head injuries (rCHI) from activities like contact sports, falls, military combat, and traffic accidents pose a serious risk due to their cumulative impact on the brain. Often, rCHI is not diagnosed until symptoms of irreversible brain damage appear, highlighting the need for...
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Nature Publishing Group
2025-01-01
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| Series: | Translational Psychiatry |
| Online Access: | https://doi.org/10.1038/s41398-025-03228-3 |
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| author | Chongyun Wu Meng Li Zhe Chen Shu Feng Qianting Deng Rui Duan Timon Cheng-Yi Liu Luodan Yang |
| author_facet | Chongyun Wu Meng Li Zhe Chen Shu Feng Qianting Deng Rui Duan Timon Cheng-Yi Liu Luodan Yang |
| author_sort | Chongyun Wu |
| collection | DOAJ |
| description | Abstract Repeated closed-head injuries (rCHI) from activities like contact sports, falls, military combat, and traffic accidents pose a serious risk due to their cumulative impact on the brain. Often, rCHI is not diagnosed until symptoms of irreversible brain damage appear, highlighting the need for preventive measures. This study assessed the prophylactic efficacy of remote photobiomodulation (PBM) targeted at the lungs against rCHI-induced brain injury and associated behavioral deficits. Utilizing the “Marmarou” weight-drop model, rCHI was induced in rats on days 0, 5, and 10. Remote PBM, employing an 808 nm continuous wave laser, was administered daily in 2-min sessions per lung side over 20 days. Behavioral deficits were assessed through three-chamber social interaction, forced swim, grip strength, open field, elevated plus maze, and Barnes maze tests. Immunofluorescence staining and 3D reconstruction evaluated neuronal damage, apoptosis, degeneration, and the morphology of microglia and astrocytes, as well as astrocyte and microglia-mediated excessive synapse elimination. Additionally, 16S rDNA amplicon sequencing analyzed changes in the lung microbiome following remote PBM treatment. Results demonstrated that remote PBM significantly improved depressive-like behaviors, motor dysfunction, and social interaction impairment while enhancing grip strength and reducing neuronal damage, apoptosis, and degeneration induced by rCHI. Analysis of lung microbiome changes revealed an enrichment of lipopolysaccharide (LPS) biosynthesis pathways, suggesting a potential link to neuroprotection. Furthermore, remote PBM mitigated hyperactivation of cortical microglia and astrocytes and significantly reduced excessive synaptic phagocytosis by these cells, highlighting its potential as a preventive strategy for rCHI with neuroprotective effects. |
| format | Article |
| id | doaj-art-19afe72cb0584110a5583f4bcb03a3f1 |
| institution | Kabale University |
| issn | 2158-3188 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Translational Psychiatry |
| spelling | doaj-art-19afe72cb0584110a5583f4bcb03a3f12025-01-12T12:40:53ZengNature Publishing GroupTranslational Psychiatry2158-31882025-01-0115111210.1038/s41398-025-03228-3Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injuryChongyun Wu0Meng Li1Zhe Chen2Shu Feng3Qianting Deng4Rui Duan5Timon Cheng-Yi Liu6Luodan Yang7Laboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Laser Sports Medicine, School of Physical Education and Sports Science, South China Normal UniversityLaboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal UniversityAbstract Repeated closed-head injuries (rCHI) from activities like contact sports, falls, military combat, and traffic accidents pose a serious risk due to their cumulative impact on the brain. Often, rCHI is not diagnosed until symptoms of irreversible brain damage appear, highlighting the need for preventive measures. This study assessed the prophylactic efficacy of remote photobiomodulation (PBM) targeted at the lungs against rCHI-induced brain injury and associated behavioral deficits. Utilizing the “Marmarou” weight-drop model, rCHI was induced in rats on days 0, 5, and 10. Remote PBM, employing an 808 nm continuous wave laser, was administered daily in 2-min sessions per lung side over 20 days. Behavioral deficits were assessed through three-chamber social interaction, forced swim, grip strength, open field, elevated plus maze, and Barnes maze tests. Immunofluorescence staining and 3D reconstruction evaluated neuronal damage, apoptosis, degeneration, and the morphology of microglia and astrocytes, as well as astrocyte and microglia-mediated excessive synapse elimination. Additionally, 16S rDNA amplicon sequencing analyzed changes in the lung microbiome following remote PBM treatment. Results demonstrated that remote PBM significantly improved depressive-like behaviors, motor dysfunction, and social interaction impairment while enhancing grip strength and reducing neuronal damage, apoptosis, and degeneration induced by rCHI. Analysis of lung microbiome changes revealed an enrichment of lipopolysaccharide (LPS) biosynthesis pathways, suggesting a potential link to neuroprotection. Furthermore, remote PBM mitigated hyperactivation of cortical microglia and astrocytes and significantly reduced excessive synaptic phagocytosis by these cells, highlighting its potential as a preventive strategy for rCHI with neuroprotective effects.https://doi.org/10.1038/s41398-025-03228-3 |
| spellingShingle | Chongyun Wu Meng Li Zhe Chen Shu Feng Qianting Deng Rui Duan Timon Cheng-Yi Liu Luodan Yang Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury Translational Psychiatry |
| title | Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| title_full | Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| title_fullStr | Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| title_full_unstemmed | Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| title_short | Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| title_sort | remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury |
| url | https://doi.org/10.1038/s41398-025-03228-3 |
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