Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis
Inflammatory periodontal diseases, despite all the efforts of modern dentistry, remain an important predictor of tooth loss worldwide. Oxidative stress plays a crucial role in the pathogenesis of periodontitis, making the use of antioxidants an attractive option for its treatment. Our attention was...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/3/186 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849342036403027968 |
|---|---|
| author | Valeriy Salnykov Igor Belenichev Lyudmyla Makyeyeva Dmytro Skoryna Valentyn Oksenych Oleksandr Kamyshnyi |
| author_facet | Valeriy Salnykov Igor Belenichev Lyudmyla Makyeyeva Dmytro Skoryna Valentyn Oksenych Oleksandr Kamyshnyi |
| author_sort | Valeriy Salnykov |
| collection | DOAJ |
| description | Inflammatory periodontal diseases, despite all the efforts of modern dentistry, remain an important predictor of tooth loss worldwide. Oxidative stress plays a crucial role in the pathogenesis of periodontitis, making the use of antioxidants an attractive option for its treatment. Our attention was drawn to the selenium compound Selenase as an antioxidant therapeutic agent. In this study, we modeled a calcium-deficient prooxidant chronic generalized periodontitis (CGP) model in white non-linear rats. Then, after 14 days, Selenase (50 μg/kg) and Mexidol (250 mg/kg) were administered intragastrically. Blood samples from the animals were analyzed using ELISA and biochemical methods to determine Cu-Zn SOD, nitrotyrosine, GPX-4, iNOS, NO<sub>x</sub>, GSH, and GSSG levels. The CGP model led to the typical clinical signs of periodontitis, including hyperemia, edema, gingival pocket formation, bleeding, tooth mobility, as well as an increase in molecular–biochemical markers of nitrosative stress and a reduction of endogenous antioxidants in the blood. Selenase resulted in a decrease in the clinical manifestations of CGP, reduced iNOS, nitrotyrosine, and NO<sub>x</sub> levels, and an increase in Cu-Zn SOD and GPX-4 compared to the control group (<i>p</i> < 0.05). Mexidol had a less pronounced effect on these markers compared to Selenase (<i>p</i> < 0.05). |
| format | Article |
| id | doaj-art-19a93d9de3de4333bafcb496e36498f9 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-19a93d9de3de4333bafcb496e36498f92025-08-20T03:43:30ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-03-0147318610.3390/cimb47030186Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized PeriodontitisValeriy Salnykov0Igor Belenichev1Lyudmyla Makyeyeva2Dmytro Skoryna3Valentyn Oksenych4Oleksandr Kamyshnyi5Department of Surgical and Propaedeutic Dentistry, Zaporizhzhia State Medical and Pharmaceutical University, 69035 Zaporizhzhia, UkraineDepartment of Pharmacology and Medical Formulation with Course of Normal Physiology, Zaporizhzhia State Medical and Pharmaceutical University, 69035 Zaporizhzhia, UkraineDepartment of Histology, Cytology and Embryology, State Medical and Pharmaceutical University, 69035 Zaporizhzhia, UkraineDepartment of Pharmaceutical, Organic, and Bioorganic Chemistry, Zaporizhzhia State Medical and Pharmaceutical University, 69035 Zaporizhzhia, UkraineBroegelmann Research Laboratory, Department of Clinical Science, University of Bergen, 5021 Bergen, NorwayDepartment of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, UkraineInflammatory periodontal diseases, despite all the efforts of modern dentistry, remain an important predictor of tooth loss worldwide. Oxidative stress plays a crucial role in the pathogenesis of periodontitis, making the use of antioxidants an attractive option for its treatment. Our attention was drawn to the selenium compound Selenase as an antioxidant therapeutic agent. In this study, we modeled a calcium-deficient prooxidant chronic generalized periodontitis (CGP) model in white non-linear rats. Then, after 14 days, Selenase (50 μg/kg) and Mexidol (250 mg/kg) were administered intragastrically. Blood samples from the animals were analyzed using ELISA and biochemical methods to determine Cu-Zn SOD, nitrotyrosine, GPX-4, iNOS, NO<sub>x</sub>, GSH, and GSSG levels. The CGP model led to the typical clinical signs of periodontitis, including hyperemia, edema, gingival pocket formation, bleeding, tooth mobility, as well as an increase in molecular–biochemical markers of nitrosative stress and a reduction of endogenous antioxidants in the blood. Selenase resulted in a decrease in the clinical manifestations of CGP, reduced iNOS, nitrotyrosine, and NO<sub>x</sub> levels, and an increase in Cu-Zn SOD and GPX-4 compared to the control group (<i>p</i> < 0.05). Mexidol had a less pronounced effect on these markers compared to Selenase (<i>p</i> < 0.05).https://www.mdpi.com/1467-3045/47/3/186chronic generalized periodontitisSelenasenitrosative stressantioxidant system |
| spellingShingle | Valeriy Salnykov Igor Belenichev Lyudmyla Makyeyeva Dmytro Skoryna Valentyn Oksenych Oleksandr Kamyshnyi Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis Current Issues in Molecular Biology chronic generalized periodontitis Selenase nitrosative stress antioxidant system |
| title | Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis |
| title_full | Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis |
| title_fullStr | Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis |
| title_full_unstemmed | Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis |
| title_short | Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis |
| title_sort | antioxidant mechanisms of the protective action of selenase in experimental chronic generalized periodontitis |
| topic | chronic generalized periodontitis Selenase nitrosative stress antioxidant system |
| url | https://www.mdpi.com/1467-3045/47/3/186 |
| work_keys_str_mv | AT valeriysalnykov antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis AT igorbelenichev antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis AT lyudmylamakyeyeva antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis AT dmytroskoryna antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis AT valentynoksenych antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis AT oleksandrkamyshnyi antioxidantmechanismsoftheprotectiveactionofselenaseinexperimentalchronicgeneralizedperiodontitis |