Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes
Glycogen is a natural polysaccharide used as an energy storage macromolecule. The role of glycogen metabolism in type 2 diabetes mellitus has been under investigation for several years, along with its implication in cancer and cardiovascular and neurodegenerative diseases. Previous studies using pig...
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MDPI AG
2024-07-01
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| Series: | Polysaccharides |
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| author | Pandora Karakousi Maria Karayianni Evangelia D. Chrysina Stergios Pispas |
| author_facet | Pandora Karakousi Maria Karayianni Evangelia D. Chrysina Stergios Pispas |
| author_sort | Pandora Karakousi |
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| description | Glycogen is a natural polysaccharide used as an energy storage macromolecule. The role of glycogen metabolism in type 2 diabetes mellitus has been under investigation for several years, along with its implication in cancer and cardiovascular and neurodegenerative diseases. Previous studies using pig liver glycogen with rabbit muscle glycogen phosphorylase (RMGP<i>b</i>), which catalyzes the first step of glycogen degradation to glucose-1-phosphate, showed that the surface of an average glycogen molecule is covered by a total of 20 RMGP<i>b</i> dimeric molecules. In this work, we selected oyster glycogen (Glyc) to investigate its interaction with RMGP<i>b</i> by employing biophysical techniques. Dynamic, static, and electrophoretic light scattering were used to investigate the solution behaviors and structures of both the Glyc molecule itself and the formed complexes between Glyc and GP<i>b</i> at different mixing ratios. It was established that the interaction between oyster Glyc and RMGP<i>b</i> is similar to that previously reported for pig liver glycogen. Moreover, the structure of the complexed GP<i>b</i> was monitored by fluorescence and FTIR spectroscopy. |
| format | Article |
| id | doaj-art-19a3bf877ee34c62a2f4b3fbe7d994c8 |
| institution | OA Journals |
| issn | 2673-4176 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | MDPI AG |
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| series | Polysaccharides |
| spelling | doaj-art-19a3bf877ee34c62a2f4b3fbe7d994c82025-08-20T01:55:48ZengMDPI AGPolysaccharides2673-41762024-07-015322524010.3390/polysaccharides5030017Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> ComplexesPandora Karakousi0Maria Karayianni1Evangelia D. Chrysina2Stergios Pispas3Institute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 116 35 Athens, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 116 35 Athens, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 116 35 Athens, GreeceTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 116 35 Athens, GreeceGlycogen is a natural polysaccharide used as an energy storage macromolecule. The role of glycogen metabolism in type 2 diabetes mellitus has been under investigation for several years, along with its implication in cancer and cardiovascular and neurodegenerative diseases. Previous studies using pig liver glycogen with rabbit muscle glycogen phosphorylase (RMGP<i>b</i>), which catalyzes the first step of glycogen degradation to glucose-1-phosphate, showed that the surface of an average glycogen molecule is covered by a total of 20 RMGP<i>b</i> dimeric molecules. In this work, we selected oyster glycogen (Glyc) to investigate its interaction with RMGP<i>b</i> by employing biophysical techniques. Dynamic, static, and electrophoretic light scattering were used to investigate the solution behaviors and structures of both the Glyc molecule itself and the formed complexes between Glyc and GP<i>b</i> at different mixing ratios. It was established that the interaction between oyster Glyc and RMGP<i>b</i> is similar to that previously reported for pig liver glycogen. Moreover, the structure of the complexed GP<i>b</i> was monitored by fluorescence and FTIR spectroscopy.https://www.mdpi.com/2673-4176/5/3/17glycogenbiopolymerglycogen phosphorylase <i>b</i>biophysical characterizationenzyme–biopolymer complexes |
| spellingShingle | Pandora Karakousi Maria Karayianni Evangelia D. Chrysina Stergios Pispas Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes Polysaccharides glycogen biopolymer glycogen phosphorylase <i>b</i> biophysical characterization enzyme–biopolymer complexes |
| title | Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes |
| title_full | Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes |
| title_fullStr | Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes |
| title_full_unstemmed | Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes |
| title_short | Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase <i>b</i> Complexes |
| title_sort | physicochemical and spectroscopic characterization of glycogen and glycogen phosphorylase i b i complexes |
| topic | glycogen biopolymer glycogen phosphorylase <i>b</i> biophysical characterization enzyme–biopolymer complexes |
| url | https://www.mdpi.com/2673-4176/5/3/17 |
| work_keys_str_mv | AT pandorakarakousi physicochemicalandspectroscopiccharacterizationofglycogenandglycogenphosphorylaseibicomplexes AT mariakarayianni physicochemicalandspectroscopiccharacterizationofglycogenandglycogenphosphorylaseibicomplexes AT evangeliadchrysina physicochemicalandspectroscopiccharacterizationofglycogenandglycogenphosphorylaseibicomplexes AT stergiospispas physicochemicalandspectroscopiccharacterizationofglycogenandglycogenphosphorylaseibicomplexes |