Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response
The family of <i>GRAS</i> transcription factors plays an essential role in the regulation of plant development, the transmission of hormonal signals, and the adaptation to environmental stresses seen in numerous species. However, a comprehensive analysis of the <i>GRAS</i> fa...
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2025-06-01
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| author | Weijia Li Hongying Sun Zhiwen Chen Yue Zhang Jianguo Zhao |
| author_facet | Weijia Li Hongying Sun Zhiwen Chen Yue Zhang Jianguo Zhao |
| author_sort | Weijia Li |
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| description | The family of <i>GRAS</i> transcription factors plays an essential role in the regulation of plant development, the transmission of hormonal signals, and the adaptation to environmental stresses seen in numerous species. However, a comprehensive analysis of the <i>GRAS</i> family of <i>Hemerocallis citrina</i> (daylily) is lacking, despite its potential to help understand the stress content and developmental processes of the monospecies. This study identified <i>78 GRAS</i> genes (<i>HcGRAS</i>) in <i>H. citrina</i>, which were classified into 15 subfamilies based on chromosomal location, gene structure, conserved motifs, and expression patterns. An analysis of promoter regions indicated a significant presence of elements related to hormones and stress, showcasing these genes’ role in adapting to environmental pressures. The GO and KEGG analyses indicated that <i>HcGRAS</i> genes were engaged in pathways associated with developmental processes and responses to environmental stress. Notably, <i>HcGRAS38</i> was identified as a key interacting protein for SHR, SCR, and DELLA subfamily members, which suggested its central role in coordinating stress response and developmental signaling. Comparative genomic mapping with seven representative monocot and dicot species underscored evolutionary conservation and divergence in functions of the <i>GRAS</i> family. The expression profiling of 22 <i>HcGRAS</i> genes across different daylily tissues provided insights into their tissue-specific roles. This research may promote the further exploration of the functional characteristics of <i>HcGRAS</i> genes. |
| format | Article |
| id | doaj-art-19a151b03b5e4208bf2db3d60d42428e |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-19a151b03b5e4208bf2db3d60d42428e2025-08-20T02:45:33ZengMDPI AGBiology2079-77372025-06-0114777010.3390/biology14070770Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress ResponseWeijia Li0Hongying Sun1Zhiwen Chen2Yue Zhang3Jianguo Zhao4Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education, Shanxi Datong University, Datong 037009, ChinaDepartment of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaEngineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education, Shanxi Datong University, Datong 037009, ChinaCollege of Agronomy and Life Science, Shanxi Datong University, Datong 037009, ChinaEngineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education, Shanxi Datong University, Datong 037009, ChinaThe family of <i>GRAS</i> transcription factors plays an essential role in the regulation of plant development, the transmission of hormonal signals, and the adaptation to environmental stresses seen in numerous species. However, a comprehensive analysis of the <i>GRAS</i> family of <i>Hemerocallis citrina</i> (daylily) is lacking, despite its potential to help understand the stress content and developmental processes of the monospecies. This study identified <i>78 GRAS</i> genes (<i>HcGRAS</i>) in <i>H. citrina</i>, which were classified into 15 subfamilies based on chromosomal location, gene structure, conserved motifs, and expression patterns. An analysis of promoter regions indicated a significant presence of elements related to hormones and stress, showcasing these genes’ role in adapting to environmental pressures. The GO and KEGG analyses indicated that <i>HcGRAS</i> genes were engaged in pathways associated with developmental processes and responses to environmental stress. Notably, <i>HcGRAS38</i> was identified as a key interacting protein for SHR, SCR, and DELLA subfamily members, which suggested its central role in coordinating stress response and developmental signaling. Comparative genomic mapping with seven representative monocot and dicot species underscored evolutionary conservation and divergence in functions of the <i>GRAS</i> family. The expression profiling of 22 <i>HcGRAS</i> genes across different daylily tissues provided insights into their tissue-specific roles. This research may promote the further exploration of the functional characteristics of <i>HcGRAS</i> genes.https://www.mdpi.com/2079-7737/14/7/770<i>Hemerocallis citrina</i> Baroni<i>GRAS</i> gene familygenome-wide analysisexpression pattern |
| spellingShingle | Weijia Li Hongying Sun Zhiwen Chen Yue Zhang Jianguo Zhao Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response Biology <i>Hemerocallis citrina</i> Baroni <i>GRAS</i> gene family genome-wide analysis expression pattern |
| title | Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response |
| title_full | Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response |
| title_fullStr | Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response |
| title_full_unstemmed | Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response |
| title_short | Genome-Wide Identification of <i>GRAS</i> Gene Family in Daylily (<i>Hemerocallis citrina</i> Baroni) and Its Expression Profiles in Development, Hormone and Biotic Stress Response |
| title_sort | genome wide identification of i gras i gene family in daylily i hemerocallis citrina i baroni and its expression profiles in development hormone and biotic stress response |
| topic | <i>Hemerocallis citrina</i> Baroni <i>GRAS</i> gene family genome-wide analysis expression pattern |
| url | https://www.mdpi.com/2079-7737/14/7/770 |
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