Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world

Abstract The Ki-67 index, which is a proliferative index, has become more important in making treatment decisions for patients with breast cancer (BC) and plays both a predictive role and a prognostic role. However, a few factors limit its use in clinical practice, particularly the assessment of the...

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Main Authors: Changsong Wang, JingChang Chen, Xuexia Lv, Tian Yun, Yaxi Wang, Nianlong Meng, Fulin Li, Yansha Cao, Naijun Fan, Xiaoyue Wang
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Cancer
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Online Access:https://doi.org/10.1186/s12885-025-14374-8
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author Changsong Wang
JingChang Chen
Xuexia Lv
Tian Yun
Yaxi Wang
Nianlong Meng
Fulin Li
Yansha Cao
Naijun Fan
Xiaoyue Wang
author_facet Changsong Wang
JingChang Chen
Xuexia Lv
Tian Yun
Yaxi Wang
Nianlong Meng
Fulin Li
Yansha Cao
Naijun Fan
Xiaoyue Wang
author_sort Changsong Wang
collection DOAJ
description Abstract The Ki-67 index, which is a proliferative index, has become more important in making treatment decisions for patients with breast cancer (BC) and plays both a predictive role and a prognostic role. However, a few factors limit its use in clinical practice, particularly the assessment of the percentage of Ki-67-positive cells and the cutoff value of Ki-67. In this study, we examined the expression of Ki-67 via immunohistochemistry and systematically evaluated the value of the Ki-67 index in patients with BC. This was a retrospective study including 280 patients diagnosed with BC. There were marked differences in overall survival (OS) between patients with BC when the Ki-67 index ranged from 46 to 68% (χ2 = 5.87, P = 0.0154; χ2 = 7.64, P = 0.0057, respectively), and the same results were also found when the staining density was added to the Ki-67 index; however, the staining density alone has limited value in assessing the value of Ki-67. There were marked differences in disease-free survival (DFS) among BC patients when the Ki-67 index ranged from 50 to 58% (χ2 = 7.31, P = 0.0069; χ2 = 7.88, P = 0.005). When 14% was used as a cutoff point to classify the molecular type of BC, the luminal A-type patients were significantly different from patients with HER2-overexpressing subtype BC in terms of OS (χ2 = 5.33, P = 0.021). There was a significant difference in the OS of patients with human epidermal growth factor receptor 2 (HER-2)-overexpressing subtype BC when the Ki-67 index fell within the range of 49-60% (χ2 = 4.86, P = 0.0275; χ2 = 5.50, P = 0.019, respectively). There were significant differences between luminal A-type BC and HER2-overexpressing subtype BC in terms of OS (χ2 = 5.53, P = 0.019), according to suggestions of the 2019 CSCO consensus. There were significant differences between the two groups of luminal B HER-2(-) BC when the Ki-67 index was 52% (χ2 = 6.61, P = 0.0101). The differentiated Ki-67 index can be used to assess the OS and DFS of patients with BC, and the staining density of Ki-67 has little value in assessing prognosis in these patients. Different molecular classification methods may influence the assessment of prognosis and the results of molecular subtype in patients with BC. To predict the prognosis of BC patients, it is more scientifically feasible to use the interval values of Ki-67 than a specific value.
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publishDate 2025-05-01
publisher BMC
record_format Article
series BMC Cancer
spelling doaj-art-199f07044fc74cac907c3e40f288025e2025-08-20T03:16:55ZengBMCBMC Cancer1471-24072025-05-0125111110.1186/s12885-025-14374-8Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real worldChangsong Wang0JingChang Chen1Xuexia Lv2Tian Yun3Yaxi Wang4Nianlong Meng5Fulin Li6Yansha Cao7Naijun Fan8Xiaoyue Wang9Department of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalSchool of Nursing, Henan University of Science and TechnologyDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalDepartment of Pathology, People’s Liberation Army Joint Logistic Support Force 989 th HospitalAbstract The Ki-67 index, which is a proliferative index, has become more important in making treatment decisions for patients with breast cancer (BC) and plays both a predictive role and a prognostic role. However, a few factors limit its use in clinical practice, particularly the assessment of the percentage of Ki-67-positive cells and the cutoff value of Ki-67. In this study, we examined the expression of Ki-67 via immunohistochemistry and systematically evaluated the value of the Ki-67 index in patients with BC. This was a retrospective study including 280 patients diagnosed with BC. There were marked differences in overall survival (OS) between patients with BC when the Ki-67 index ranged from 46 to 68% (χ2 = 5.87, P = 0.0154; χ2 = 7.64, P = 0.0057, respectively), and the same results were also found when the staining density was added to the Ki-67 index; however, the staining density alone has limited value in assessing the value of Ki-67. There were marked differences in disease-free survival (DFS) among BC patients when the Ki-67 index ranged from 50 to 58% (χ2 = 7.31, P = 0.0069; χ2 = 7.88, P = 0.005). When 14% was used as a cutoff point to classify the molecular type of BC, the luminal A-type patients were significantly different from patients with HER2-overexpressing subtype BC in terms of OS (χ2 = 5.33, P = 0.021). There was a significant difference in the OS of patients with human epidermal growth factor receptor 2 (HER-2)-overexpressing subtype BC when the Ki-67 index fell within the range of 49-60% (χ2 = 4.86, P = 0.0275; χ2 = 5.50, P = 0.019, respectively). There were significant differences between luminal A-type BC and HER2-overexpressing subtype BC in terms of OS (χ2 = 5.53, P = 0.019), according to suggestions of the 2019 CSCO consensus. There were significant differences between the two groups of luminal B HER-2(-) BC when the Ki-67 index was 52% (χ2 = 6.61, P = 0.0101). The differentiated Ki-67 index can be used to assess the OS and DFS of patients with BC, and the staining density of Ki-67 has little value in assessing prognosis in these patients. Different molecular classification methods may influence the assessment of prognosis and the results of molecular subtype in patients with BC. To predict the prognosis of BC patients, it is more scientifically feasible to use the interval values of Ki-67 than a specific value.https://doi.org/10.1186/s12885-025-14374-8Ki-67Breast CancerSurviveMolecular TypePrognosis
spellingShingle Changsong Wang
JingChang Chen
Xuexia Lv
Tian Yun
Yaxi Wang
Nianlong Meng
Fulin Li
Yansha Cao
Naijun Fan
Xiaoyue Wang
Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
BMC Cancer
Ki-67
Breast Cancer
Survive
Molecular Type
Prognosis
title Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
title_full Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
title_fullStr Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
title_full_unstemmed Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
title_short Ki-67—Playing a key role in breast cancer but difficult to apply precisely in the real world
title_sort ki 67 playing a key role in breast cancer but difficult to apply precisely in the real world
topic Ki-67
Breast Cancer
Survive
Molecular Type
Prognosis
url https://doi.org/10.1186/s12885-025-14374-8
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