Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study

Abstract Laryngeal squamous cell carcinoma (LSCC) presents significant treatment challenges, especially regarding recurrence after larynx-preservation therapy. We identified distinct microbial community structures between recurrence and non-recurrence groups, particularly highlighting the genera abu...

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Main Authors: Chi-Yao Hsueh, Xiaohui Yuan, Huiying Huang, Yujie Shen, Qiang Huang, Weida Dong, Danhui Li, Hui-Ching Lau, Xinhui Mao, Simin Liang, Lei Tao, Ming Zhang, Ji Sun, Hongli Gong
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:npj Biofilms and Microbiomes
Online Access:https://doi.org/10.1038/s41522-025-00789-5
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author Chi-Yao Hsueh
Xiaohui Yuan
Huiying Huang
Yujie Shen
Qiang Huang
Weida Dong
Danhui Li
Hui-Ching Lau
Xinhui Mao
Simin Liang
Lei Tao
Ming Zhang
Ji Sun
Hongli Gong
author_facet Chi-Yao Hsueh
Xiaohui Yuan
Huiying Huang
Yujie Shen
Qiang Huang
Weida Dong
Danhui Li
Hui-Ching Lau
Xinhui Mao
Simin Liang
Lei Tao
Ming Zhang
Ji Sun
Hongli Gong
author_sort Chi-Yao Hsueh
collection DOAJ
description Abstract Laryngeal squamous cell carcinoma (LSCC) presents significant treatment challenges, especially regarding recurrence after larynx-preservation therapy. We identified distinct microbial community structures between recurrence and non-recurrence groups, particularly highlighting the genera abundance of Fusobacterium and Serratia. However, larynx-preserving therapy did not significantly alter microbial diversity in recurrent patients. Survival analysis identified Fusobacterium and Serratia as independent prognostic factors for recurrence, leading to the development of a Serratia-Fusobacterium (SF) prognostic scoring model. The SF model achieved an AUC of 81.37% for predicting recurrence, outperforming the TNM staging system. LSCC patients classified as high-risk by the SF model exhibited significantly shorter disease-free survival (DFS) compared to low-risk patients in the LSCC cohort. Furthermore, the SF model demonstrated an AUC of 78.48% in the multi-center cohort for predicting recurrence. In conclusion, the Serratia-Fusobacterium prognostic scoring model can predict LSCC recurrence after larynx-preserving therapy and provide valuable insights to inform recommendations for LSCC surveillance.
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spelling doaj-art-199817a8427341c4a60d0ec3d2ff34662025-08-20T03:42:40ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-08-0111111010.1038/s41522-025-00789-5Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control studyChi-Yao Hsueh0Xiaohui Yuan1Huiying Huang2Yujie Shen3Qiang Huang4Weida Dong5Danhui Li6Hui-Ching Lau7Xinhui Mao8Simin Liang9Lei Tao10Ming Zhang11Ji Sun12Hongli Gong13ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityDepartment of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Pathology, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityDepartment of Pathology, Eye & ENT Hospital, Fudan UniversityENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityAbstract Laryngeal squamous cell carcinoma (LSCC) presents significant treatment challenges, especially regarding recurrence after larynx-preservation therapy. We identified distinct microbial community structures between recurrence and non-recurrence groups, particularly highlighting the genera abundance of Fusobacterium and Serratia. However, larynx-preserving therapy did not significantly alter microbial diversity in recurrent patients. Survival analysis identified Fusobacterium and Serratia as independent prognostic factors for recurrence, leading to the development of a Serratia-Fusobacterium (SF) prognostic scoring model. The SF model achieved an AUC of 81.37% for predicting recurrence, outperforming the TNM staging system. LSCC patients classified as high-risk by the SF model exhibited significantly shorter disease-free survival (DFS) compared to low-risk patients in the LSCC cohort. Furthermore, the SF model demonstrated an AUC of 78.48% in the multi-center cohort for predicting recurrence. In conclusion, the Serratia-Fusobacterium prognostic scoring model can predict LSCC recurrence after larynx-preserving therapy and provide valuable insights to inform recommendations for LSCC surveillance.https://doi.org/10.1038/s41522-025-00789-5
spellingShingle Chi-Yao Hsueh
Xiaohui Yuan
Huiying Huang
Yujie Shen
Qiang Huang
Weida Dong
Danhui Li
Hui-Ching Lau
Xinhui Mao
Simin Liang
Lei Tao
Ming Zhang
Ji Sun
Hongli Gong
Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
npj Biofilms and Microbiomes
title Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
title_full Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
title_fullStr Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
title_full_unstemmed Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
title_short Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study
title_sort evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma a multicenter case control study
url https://doi.org/10.1038/s41522-025-00789-5
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