Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine

Abstract This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlorite (NaOCl) using conventional irrigation (CI). Teeth were distributed...

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Main Authors: Julia Menezes Savaris, Maria Eduarda Paz Dotto, Lucas da Fonseca Roberti Garcia, Emmanuel João Nogueira Leal da Silva, Bruno Alexandre Pacheco de Castro Henriques, Cleonice da Silveira Teixeira, Eduardo Antunes Bortoluzzi
Format: Article
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica 2024-12-01
Series:Brazilian Oral Research
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242024000101107&lng=en&tlng=en
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author Julia Menezes Savaris
Maria Eduarda Paz Dotto
Lucas da Fonseca Roberti Garcia
Emmanuel João Nogueira Leal da Silva
Bruno Alexandre Pacheco de Castro Henriques
Cleonice da Silveira Teixeira
Eduardo Antunes Bortoluzzi
author_facet Julia Menezes Savaris
Maria Eduarda Paz Dotto
Lucas da Fonseca Roberti Garcia
Emmanuel João Nogueira Leal da Silva
Bruno Alexandre Pacheco de Castro Henriques
Cleonice da Silveira Teixeira
Eduardo Antunes Bortoluzzi
author_sort Julia Menezes Savaris
collection DOAJ
description Abstract This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlorite (NaOCl) using conventional irrigation (CI). Teeth were distributed into four groups based on final irrigation protocols: Control Group (CG): 17%EDTA/CI + H2O; G1: 2.5%NaOCl/Passive Ultrasonic Irrigation (PUI) + EDTA/PUI + NaOCl/PUI (60s each); G2: EDTA/PUI + NaOCl/PUI (30s each); G3: EDTA/PUI + NaOCl/PUI + H2O/PUI + CHX/PUI (30s each). Four tests were conducted: three-point flexural strength test, Scanning Electron Microscopy analysis, microhardness assessment, and the push-out bond strength (POBS) of the filling material to the root dentine. Data were analyzed using ANOVA and Tukey tests (Flexural strength), and Student t-test (Microhardness). Erosion scores and POBS were analyzed by Kruskal-Wallis and Dunn tests (α = 0.05). Results indicated no significant differences in flexural strength (p > 0.05) among groups. CG exhibited the lowest erosion scores in the cervical third. In the middle third, CG had lower scores than G1 and G2, while in the apical third, CG had lower scores than G1 (p < 0.05). Microhardness values decreased following the protocols (p < 0.05), except for the CG (p > 0.05). G2 displayed higher POBS values in the middle and apical thirds (p < 0.05). The protocols did not significantly influence the flexural strength of root dentine. However, they did affect microhardness and promoted greater erosion. The best results for POBS were observed when the final irrigation involved the sequential use of EDTA and NaOCl employing PUI for 30 seconds in each solution.
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spelling doaj-art-19919a3c9dc841d996c3e34eca1885cd2025-08-20T01:57:54ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072024-12-013810.1590/1807-3107bor-2024.vol38.0072Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentineJulia Menezes Savarishttps://orcid.org/0000-0002-5753-5618Maria Eduarda Paz Dottohttps://orcid.org/0000-0003-1253-1274Lucas da Fonseca Roberti Garciahttps://orcid.org/0000-0002-8724-0124Emmanuel João Nogueira Leal da Silvahttps://orcid.org/0000-0002-6445-8243Bruno Alexandre Pacheco de Castro Henriqueshttps://orcid.org/0000-0002-6472-5479Cleonice da Silveira Teixeirahttps://orcid.org/0000-0002-0139-8159Eduardo Antunes Bortoluzzihttps://orcid.org/0000-0003-4426-9143Abstract This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlorite (NaOCl) using conventional irrigation (CI). Teeth were distributed into four groups based on final irrigation protocols: Control Group (CG): 17%EDTA/CI + H2O; G1: 2.5%NaOCl/Passive Ultrasonic Irrigation (PUI) + EDTA/PUI + NaOCl/PUI (60s each); G2: EDTA/PUI + NaOCl/PUI (30s each); G3: EDTA/PUI + NaOCl/PUI + H2O/PUI + CHX/PUI (30s each). Four tests were conducted: three-point flexural strength test, Scanning Electron Microscopy analysis, microhardness assessment, and the push-out bond strength (POBS) of the filling material to the root dentine. Data were analyzed using ANOVA and Tukey tests (Flexural strength), and Student t-test (Microhardness). Erosion scores and POBS were analyzed by Kruskal-Wallis and Dunn tests (α = 0.05). Results indicated no significant differences in flexural strength (p > 0.05) among groups. CG exhibited the lowest erosion scores in the cervical third. In the middle third, CG had lower scores than G1 and G2, while in the apical third, CG had lower scores than G1 (p < 0.05). Microhardness values decreased following the protocols (p < 0.05), except for the CG (p > 0.05). G2 displayed higher POBS values in the middle and apical thirds (p < 0.05). The protocols did not significantly influence the flexural strength of root dentine. However, they did affect microhardness and promoted greater erosion. The best results for POBS were observed when the final irrigation involved the sequential use of EDTA and NaOCl employing PUI for 30 seconds in each solution.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242024000101107&lng=en&tlng=enEndodonticsUltrasonicsRoot Canal IrrigantsDentin
spellingShingle Julia Menezes Savaris
Maria Eduarda Paz Dotto
Lucas da Fonseca Roberti Garcia
Emmanuel João Nogueira Leal da Silva
Bruno Alexandre Pacheco de Castro Henriques
Cleonice da Silveira Teixeira
Eduardo Antunes Bortoluzzi
Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
Brazilian Oral Research
Endodontics
Ultrasonics
Root Canal Irrigants
Dentin
title Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
title_full Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
title_fullStr Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
title_full_unstemmed Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
title_short Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
title_sort effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine
topic Endodontics
Ultrasonics
Root Canal Irrigants
Dentin
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242024000101107&lng=en&tlng=en
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