Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line
The created cell line of human proximal renal tubules RPTEC/TERT1 showed maximum compliance with primary human RPTEC, which makes it optimal for use in studies of the nephrotoxic properties of xenobiotics. Transepithelial resistance measurement (TEER) is a valuable non-invasive method that can be us...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2024-01-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/1075 |
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| author | V. A. Evteev I. S. Semenova N. D. Bunyatyan A. B. Prokofiev V. G. Kukes |
| author_facet | V. A. Evteev I. S. Semenova N. D. Bunyatyan A. B. Prokofiev V. G. Kukes |
| author_sort | V. A. Evteev |
| collection | DOAJ |
| description | The created cell line of human proximal renal tubules RPTEC/TERT1 showed maximum compliance with primary human RPTEC, which makes it optimal for use in studies of the nephrotoxic properties of xenobiotics. Transepithelial resistance measurement (TEER) is a valuable non-invasive method that can be used to quantify the integrity of the cell barrier at various stages of cell growth and differentiation, as well as to predict the toxicity and permeability of drugs.The aim of the study was to examine the dynamics of changes in transepithelial resistance in the model of the RPTEC/TERT1 cell line during incubation with drugs with nephrotoxic effects.Material and methods. The human proximal renal tubule cell line RPTEC/TERT was obtained from the ATCC cell culture bank. Cells at passage 14 were used in the experiment. The following drugs were studied: cisplatin: 2.5 mcg/ml; vancomycin: 50 mcg/ml; doripenem — 20mcg/ml; cefepim — 150mcg/ml. 4–5 repetitions were performed for each concentration of the drug in the experiment. THEER measurements were carried out 4–5 times for each well.Results. Cisplatin, vancomycin, doripenem, and cefepim in the concentrations used do not show a cytotoxic effect on the RPTEC cell line according to TEER dynamics. |
| format | Article |
| id | doaj-art-198e692a97c5411991524a972ddefc21 |
| institution | DOAJ |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2024-01-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-198e692a97c5411991524a972ddefc212025-08-20T03:20:32ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902024-01-01689-10424510.37489/0235-2990-2023-68-9-10-42-45991Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell LineV. A. Evteev0I. S. Semenova1N. D. Bunyatyan2A. B. Prokofiev3V. G. Kukes4Scientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian FederationScientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian FederationScientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation; I. M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian FederationScientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation; I. M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian FederationScientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian FederationThe created cell line of human proximal renal tubules RPTEC/TERT1 showed maximum compliance with primary human RPTEC, which makes it optimal for use in studies of the nephrotoxic properties of xenobiotics. Transepithelial resistance measurement (TEER) is a valuable non-invasive method that can be used to quantify the integrity of the cell barrier at various stages of cell growth and differentiation, as well as to predict the toxicity and permeability of drugs.The aim of the study was to examine the dynamics of changes in transepithelial resistance in the model of the RPTEC/TERT1 cell line during incubation with drugs with nephrotoxic effects.Material and methods. The human proximal renal tubule cell line RPTEC/TERT was obtained from the ATCC cell culture bank. Cells at passage 14 were used in the experiment. The following drugs were studied: cisplatin: 2.5 mcg/ml; vancomycin: 50 mcg/ml; doripenem — 20mcg/ml; cefepim — 150mcg/ml. 4–5 repetitions were performed for each concentration of the drug in the experiment. THEER measurements were carried out 4–5 times for each well.Results. Cisplatin, vancomycin, doripenem, and cefepim in the concentrations used do not show a cytotoxic effect on the RPTEC cell line according to TEER dynamics.https://www.antibiotics-chemotherapy.ru/jour/article/view/1075antibioticscell linesnephrotoxicitytransepithelial resistance |
| spellingShingle | V. A. Evteev I. S. Semenova N. D. Bunyatyan A. B. Prokofiev V. G. Kukes Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line Антибиотики и Химиотерапия antibiotics cell lines nephrotoxicity transepithelial resistance |
| title | Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line |
| title_full | Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line |
| title_fullStr | Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line |
| title_full_unstemmed | Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line |
| title_short | Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line |
| title_sort | study of antibacterial drugs nephrotoxicity in the rptec cell line |
| topic | antibiotics cell lines nephrotoxicity transepithelial resistance |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/1075 |
| work_keys_str_mv | AT vaevteev studyofantibacterialdrugsnephrotoxicityintherpteccellline AT issemenova studyofantibacterialdrugsnephrotoxicityintherpteccellline AT ndbunyatyan studyofantibacterialdrugsnephrotoxicityintherpteccellline AT abprokofiev studyofantibacterialdrugsnephrotoxicityintherpteccellline AT vgkukes studyofantibacterialdrugsnephrotoxicityintherpteccellline |