Comparative morphological characteristics for stable and progressive pulmonary tuberculoma
The objective of the study was to identify a leading link in local and lung neuroendocrine immune during tuberculoma. Materials and methods. 60 cases of stable and progressive tuberculoma were studied. The study was conducted in 2 anatomical zones: tuberculoma capsule and adjacent areas. A compariso...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Sankt-Peterburg : NIIÈM imeni Pastera
2025-04-01
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| Series: | Инфекция и иммунитет |
| Subjects: | |
| Online Access: | https://iimmun.ru/iimm/article/viewFile/17638/2067 |
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| Summary: | The objective of the study was to identify a leading link in local and lung neuroendocrine immune during tuberculoma. Materials and methods. 60 cases of stable and progressive tuberculoma were studied. The study was conducted in 2 anatomical zones: tuberculoma capsule and adjacent areas. A comparison (apparently healthy) group contained resected samples of the upper lobes in the right lung collected from 10 men died in an accident lacking tuberculosis over lifespan. An immunohistochemistry with monoclonal antibodies against CD8 and CD4 markers was used to assess local immune response. Lung catecholamine level was analyzed by the Falk–Hillarp luminescent histochemical method. Results. When assessing localized lung expression of the studied markers in “apparently healthy” group, the number of CD4+ and CD8+ cells was observed mainly in the interstitial space: the former prevailed over the latter. In stable tuberculoma, higher lymphocyte number in the capsule was revealed that dominated by T-killers vs T-helpers. In the perifocal zone of stable tuberculoma vs apparently healthy group, the number of immune cells decreased, but at the same time zones where CD4+ lymphocytes form contacts with interstitial macrophages were detected. In the capsule of progressive tuberculoma, the number of CD4+ and CD8+ cells increased, containing more T-killers than T-helpers. In the perifocal zone, there is a decrease in CD4+ T lymphocyte number paralleled with increase in CD8+ cells. The number of intraalveolar and interstitial macrophages sometimes contacting CD4+ lymphocytes increases. Cytotoxic lymphocytes concentrate around blood vessels. When studying catecholamine level, it was found to increase in mast cells in all studied areas during stable and progressive tuberculoma. In macrophages, the catecholamine level decreases in stable disease, and increases sharply in progressive progressive course. Conclusion. The regulation of the phases of tuberculous inflammation course is carried out by various cellular interactions, depends on mycobacteria load in lung tissue and macrophage catecholamine level. |
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| ISSN: | 2220-7619 2313-7398 |