The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella
Abstract Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood....
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BMC
2024-12-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-024-01431-6 |
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| author | Pei Sun Chaoyue Wang Fujie Xie Linlin Chen Yuanyuan Zhang Xinming Tang Dandan Hu Yang Gao Ning Zhang Zhenkai Hao Yonglan Yu Jingxia Suo Xun Suo Xianyong Liu |
| author_facet | Pei Sun Chaoyue Wang Fujie Xie Linlin Chen Yuanyuan Zhang Xinming Tang Dandan Hu Yang Gao Ning Zhang Zhenkai Hao Yonglan Yu Jingxia Suo Xun Suo Xianyong Liu |
| author_sort | Pei Sun |
| collection | DOAJ |
| description | Abstract Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies. |
| format | Article |
| id | doaj-art-1978fc31a5054ebfa2e9f148ff058607 |
| institution | DOAJ |
| issn | 1297-9716 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-1978fc31a5054ebfa2e9f148ff0586072025-08-20T02:39:41ZengBMCVeterinary Research1297-97162024-12-0155111410.1186/s13567-024-01431-6The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenellaPei Sun0Chaoyue Wang1Fujie Xie2Linlin Chen3Yuanyuan Zhang4Xinming Tang5Dandan Hu6Yang Gao7Ning Zhang8Zhenkai Hao9Yonglan Yu10Jingxia Suo11Xun Suo12Xianyong Liu13National Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityDepartment of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture & Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural UniversityKey Laboratory of Animal Biosafety Risk Prevention and Control (North) of MARA, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesCollege of Animal Science and Technology, Guangxi UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityDepartment of Clinic Veterinary Medicine, College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityAbstract Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies.https://doi.org/10.1186/s13567-024-01431-6Eimeria tenellasalinomycindrug resistancepoint mutation |
| spellingShingle | Pei Sun Chaoyue Wang Fujie Xie Linlin Chen Yuanyuan Zhang Xinming Tang Dandan Hu Yang Gao Ning Zhang Zhenkai Hao Yonglan Yu Jingxia Suo Xun Suo Xianyong Liu The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella Veterinary Research Eimeria tenella salinomycin drug resistance point mutation |
| title | The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella |
| title_full | The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella |
| title_fullStr | The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella |
| title_full_unstemmed | The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella |
| title_short | The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella |
| title_sort | f204s mutation in adrenodoxin oxidoreductase drives salinomycin resistance in eimeria tenella |
| topic | Eimeria tenella salinomycin drug resistance point mutation |
| url | https://doi.org/10.1186/s13567-024-01431-6 |
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