Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey
<b>Aim:</b> In the present study, DGAT1 gene K232A and promoter and intron 1 indel polymorphisms of PRNP gene were studied.<p> <b>Materials and Methods:</b> Samples were collected a total of 122 animals including Turkish Grey (AG), Anatolian Black (AB), South Anatolian...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1147 |
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| author | İclal Şahin Zafer Bulut Ercan Kurar Yusuf Özşensoy Müge Doğan Mehmet Nizamlıoğlu |
| author_facet | İclal Şahin Zafer Bulut Ercan Kurar Yusuf Özşensoy Müge Doğan Mehmet Nizamlıoğlu |
| author_sort | İclal Şahin |
| collection | DOAJ |
| description | <b>Aim:</b> In the present study, DGAT1 gene K232A and promoter and
intron 1 indel polymorphisms of PRNP gene were studied.<p>
<b>Materials and Methods:</b> Samples were collected a total of 122
animals including Turkish Grey (AG), Anatolian Black (AB), South
Anatolian Red (SAR), East Anatolian Red (EAR) ve Zavot breeds
were used for the study. The samples were genotyped for
DGAT1 K232A polymorphism (A and K allel) using the polymerase
chain reaction-restriction fragment lenght polymorphisms
(PCR-RFLP) technique. PRNP gene polymorphism in the promoter
(23 bp indel) and intron 1 regions (12 bp indel) were determined
by PCR analysis.<p>
<b>Results:</b> The highest frequency of A allele in DGAT1 gene was
found in the AG (0.58), followed by SAR and Zavot (0.50), AG and
EAR showed the lowest frequencies of A allele (0.38). The highest
frequency of del/del genotype in promoter region of PRNP
gene was found in the EAR and SAR (0.53 and 0.52, respectively)
followed by AG (0.46); however, AB (0.19) and Zavot (0.17) showed
the low frequencies. In intron 1 region of PRNP, the highest
frequency of del/del genotype was found in the AG (0.25) and
EAR (0.13), followed by AB (0.08). The lowest frequency (0.05)
of del/del genotype was observed in EAR and Zavot.<p>
<b>Conclusion:</b> Results of the present study on AG, AB, SAR, EAR
and Zavot breeds could be used to guide association studies between
DGAT1 and PRNP gene polymorphisms may be useful for
selection of milk traits on animal husbandry and reclamation
studies. |
| format | Article |
| id | doaj-art-197775fbe6de4d9598b78b809647286d |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-197775fbe6de4d9598b78b809647286d2025-01-02T22:36:19ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195333120251147Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkeyİclal Şahin0Zafer Bulut1Ercan Kurar2Yusuf Özşensoy3Müge Doğan4Mehmet Nizamlıoğlu5Selçuk üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampüs, Konya, TürkiyeNecmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Meram, Konya, TürkiyeCumhuriyet Üniversitesi, Veteriner Fakültesi, Biyometri ve Genetik Anabilim Dalı, Sivas, TürkiyeKonya Veteriner Kontrol Enstitüsü Müdürlüğü, Konya, TürkiyeSelçuk üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampüs, Konya, Türkiye<b>Aim:</b> In the present study, DGAT1 gene K232A and promoter and intron 1 indel polymorphisms of PRNP gene were studied.<p> <b>Materials and Methods:</b> Samples were collected a total of 122 animals including Turkish Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR) ve Zavot breeds were used for the study. The samples were genotyped for DGAT1 K232A polymorphism (A and K allel) using the polymerase chain reaction-restriction fragment lenght polymorphisms (PCR-RFLP) technique. PRNP gene polymorphism in the promoter (23 bp indel) and intron 1 regions (12 bp indel) were determined by PCR analysis.<p> <b>Results:</b> The highest frequency of A allele in DGAT1 gene was found in the AG (0.58), followed by SAR and Zavot (0.50), AG and EAR showed the lowest frequencies of A allele (0.38). The highest frequency of del/del genotype in promoter region of PRNP gene was found in the EAR and SAR (0.53 and 0.52, respectively) followed by AG (0.46); however, AB (0.19) and Zavot (0.17) showed the low frequencies. In intron 1 region of PRNP, the highest frequency of del/del genotype was found in the AG (0.25) and EAR (0.13), followed by AB (0.08). The lowest frequency (0.05) of del/del genotype was observed in EAR and Zavot.<p> <b>Conclusion:</b> Results of the present study on AG, AB, SAR, EAR and Zavot breeds could be used to guide association studies between DGAT1 and PRNP gene polymorphisms may be useful for selection of milk traits on animal husbandry and reclamation studies.http://eurasianjvetsci.org/pdf.php3?id=1147cattledgat1 geneprnp gene |
| spellingShingle | İclal Şahin Zafer Bulut Ercan Kurar Yusuf Özşensoy Müge Doğan Mehmet Nizamlıoğlu Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey Eurasian Journal of Veterinary Sciences cattle dgat1 gene prnp gene |
| title | Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| title_full | Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| title_fullStr | Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| title_full_unstemmed | Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| title_short | Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| title_sort | investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey |
| topic | cattle dgat1 gene prnp gene |
| url | http://eurasianjvetsci.org/pdf.php3?id=1147 |
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