Investigation of dgat1 and prnp gene polymorphism of various cattle breeds in turkey
<b>Aim:</b> In the present study, DGAT1 gene K232A and promoter and intron 1 indel polymorphisms of PRNP gene were studied.<p> <b>Materials and Methods:</b> Samples were collected a total of 122 animals including Turkish Grey (AG), Anatolian Black (AB), South Anatolian...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1147 |
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| Summary: | <b>Aim:</b> In the present study, DGAT1 gene K232A and promoter and
intron 1 indel polymorphisms of PRNP gene were studied.<p>
<b>Materials and Methods:</b> Samples were collected a total of 122
animals including Turkish Grey (AG), Anatolian Black (AB), South
Anatolian Red (SAR), East Anatolian Red (EAR) ve Zavot breeds
were used for the study. The samples were genotyped for
DGAT1 K232A polymorphism (A and K allel) using the polymerase
chain reaction-restriction fragment lenght polymorphisms
(PCR-RFLP) technique. PRNP gene polymorphism in the promoter
(23 bp indel) and intron 1 regions (12 bp indel) were determined
by PCR analysis.<p>
<b>Results:</b> The highest frequency of A allele in DGAT1 gene was
found in the AG (0.58), followed by SAR and Zavot (0.50), AG and
EAR showed the lowest frequencies of A allele (0.38). The highest
frequency of del/del genotype in promoter region of PRNP
gene was found in the EAR and SAR (0.53 and 0.52, respectively)
followed by AG (0.46); however, AB (0.19) and Zavot (0.17) showed
the low frequencies. In intron 1 region of PRNP, the highest
frequency of del/del genotype was found in the AG (0.25) and
EAR (0.13), followed by AB (0.08). The lowest frequency (0.05)
of del/del genotype was observed in EAR and Zavot.<p>
<b>Conclusion:</b> Results of the present study on AG, AB, SAR, EAR
and Zavot breeds could be used to guide association studies between
DGAT1 and PRNP gene polymorphisms may be useful for
selection of milk traits on animal husbandry and reclamation
studies. |
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| ISSN: | 1309-6958 2146-1953 |