<i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall
Aluminum (Al) dissolves from soil at low pH and is absorbed by plants, inhibiting their growth. Since most of the Al absorbed by plants is present in the cell wall, it is thought that the binding of Al to cell wall polysaccharides alters the properties of the cell wall and inhibits cell elongation....
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/13/1912 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849429198923366400 |
|---|---|
| author | Akane Tatsumi Teruki Nagayama Ayumi Teramoto Atsuko Nakamura Ryusuke Yokoyama Jun Furukawa Hiroaki Iwai |
| author_facet | Akane Tatsumi Teruki Nagayama Ayumi Teramoto Atsuko Nakamura Ryusuke Yokoyama Jun Furukawa Hiroaki Iwai |
| author_sort | Akane Tatsumi |
| collection | DOAJ |
| description | Aluminum (Al) dissolves from soil at low pH and is absorbed by plants, inhibiting their growth. Since most of the Al absorbed by plants is present in the cell wall, it is thought that the binding of Al to cell wall polysaccharides alters the properties of the cell wall and inhibits cell elongation. However, it remains unclear in which component of the cell wall Al accumulates. In this study, we determined the distribution of Al in rice root cell wall fractions under Al stress conditions. The results show that Al accumulates predominantly in the hemicellulose fraction, with <i>star1</i> mutants accumulating significantly more Al than WT plants. An analysis of cell wall sugars revealed an increase in xyloglucan content under Al stress, which influenced the inhibition of root elongation. <i>OsXTH19</i>, a member of the xyloglucan endotransglucosylase/hydrolase (XTH) family, exhibits only xyloglucan endohydrolase (XEH) activity and lacks endotransglucosylase (XET) activity. <i>OsXTH19</i> overexpressor rice (<i>OsXTH19-OX</i>) enhances the degradation of xyloglucan. Furthermore, <i>OsXTH19-OX</i> rice with reduced xyloglucan levels exhibited reduced Al accumulation and enhanced root growth under Al stress. |
| format | Article |
| id | doaj-art-19751091e3bb4635b9db9cfb8b3cd9a3 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-19751091e3bb4635b9db9cfb8b3cd9a32025-08-20T03:28:26ZengMDPI AGPlants2223-77472025-06-011413191210.3390/plants14131912<i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell WallAkane Tatsumi0Teruki Nagayama1Ayumi Teramoto2Atsuko Nakamura3Ryusuke Yokoyama4Jun Furukawa5Hiroaki Iwai6Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanNational Agriculture and Food Research Organization, Tsukuba 305-8602, Ibaraki, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanGraduate School of Life Sciences, Tohoku University, Sendai 980-8578, Miyagi, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanDepartment of Biology, School of Biological Sciences, Tokai University, Sapporo 005-8601, Hokkaido, JapanAluminum (Al) dissolves from soil at low pH and is absorbed by plants, inhibiting their growth. Since most of the Al absorbed by plants is present in the cell wall, it is thought that the binding of Al to cell wall polysaccharides alters the properties of the cell wall and inhibits cell elongation. However, it remains unclear in which component of the cell wall Al accumulates. In this study, we determined the distribution of Al in rice root cell wall fractions under Al stress conditions. The results show that Al accumulates predominantly in the hemicellulose fraction, with <i>star1</i> mutants accumulating significantly more Al than WT plants. An analysis of cell wall sugars revealed an increase in xyloglucan content under Al stress, which influenced the inhibition of root elongation. <i>OsXTH19</i>, a member of the xyloglucan endotransglucosylase/hydrolase (XTH) family, exhibits only xyloglucan endohydrolase (XEH) activity and lacks endotransglucosylase (XET) activity. <i>OsXTH19</i> overexpressor rice (<i>OsXTH19-OX</i>) enhances the degradation of xyloglucan. Furthermore, <i>OsXTH19-OX</i> rice with reduced xyloglucan levels exhibited reduced Al accumulation and enhanced root growth under Al stress.https://www.mdpi.com/2223-7747/14/13/1912Aluminumxyloglucan<i>OsXTH19</i><i>Oryza sativa</i> |
| spellingShingle | Akane Tatsumi Teruki Nagayama Ayumi Teramoto Atsuko Nakamura Ryusuke Yokoyama Jun Furukawa Hiroaki Iwai <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall Plants Aluminum xyloglucan <i>OsXTH19</i> <i>Oryza sativa</i> |
| title | <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall |
| title_full | <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall |
| title_fullStr | <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall |
| title_full_unstemmed | <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall |
| title_short | <i>OsXTH19</i> Overexpression Improves Aluminum Tolerance via Xyloglucan Reduction in Rice Root Cell Wall |
| title_sort | i osxth19 i overexpression improves aluminum tolerance via xyloglucan reduction in rice root cell wall |
| topic | Aluminum xyloglucan <i>OsXTH19</i> <i>Oryza sativa</i> |
| url | https://www.mdpi.com/2223-7747/14/13/1912 |
| work_keys_str_mv | AT akanetatsumi iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT terukinagayama iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT ayumiteramoto iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT atsukonakamura iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT ryusukeyokoyama iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT junfurukawa iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall AT hiroakiiwai iosxth19ioverexpressionimprovesaluminumtoleranceviaxyloglucanreductioninricerootcellwall |