Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia
Introduction: Despite the numerous studies demonstrating gut microbiota dysbiosis in obese subjects, there is no data on the association between obesity and gastric microbiota. The aim of this study was to address this gap in literature by comparing the composition of gastric microbiota in obese pa...
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The Journal of Infection in Developing Countries
2024-06-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/19304 |
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| author | Umut Gazi Gunnur Kocer Emrah Ruh Can Holyavkin Ozgur Tosun Mustafa Celik Aysegul Cort Donmez Onur Birsen |
| author_facet | Umut Gazi Gunnur Kocer Emrah Ruh Can Holyavkin Ozgur Tosun Mustafa Celik Aysegul Cort Donmez Onur Birsen |
| author_sort | Umut Gazi |
| collection | DOAJ |
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Introduction: Despite the numerous studies demonstrating gut microbiota dysbiosis in obese subjects, there is no data on the association between obesity and gastric microbiota. The aim of this study was to address this gap in literature by comparing the composition of gastric microbiota in obese patients and a control group which included normal weight volunteers diagnosed with functional dyspepsia (FD).
Methodology: A total of 19 obese patients, and 18 normal weight subjects with FD and normal endoscopy results were included in the study. The gastric tissue samples were collected from participants in both groups by bariatric surgery and endoscopy, respectively, and profiled using 16S ribosomal RNA gene sequencing.
Results: There was no significant difference in the α-diversity scores, while distinct gastric microbial compositions were detected in both groups. Significantly lower levels of Bacteroidetes and Fusobacteria, and higher Firmicutes/Bacteroidetes ratio were recorded in the obese patients. A total of 15 bacterial genera exhibited significant difference in gastric abundance with Prevotella_7, Veillonella, Cupriavidus, and Acinetobacter, present in frequencies higher than 3% in at least one subject group.
Conclusions: Our study suggests a significant association between obesity and gastric microbiome composition. Future studies with larger sample size and gastric samples from subjects without any gastrointestinal complications are required to confirm our conclusions.
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| format | Article |
| id | doaj-art-1964fd1e32424cb3965ddcbca22e49c8 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-1964fd1e32424cb3965ddcbca22e49c82025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-06-01180610.3855/jidc.19304Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsiaUmut Gazi0Gunnur Kocer1Emrah Ruh2Can Holyavkin3Ozgur Tosun4Mustafa Celik5Aysegul Cort Donmez6Onur Birsen7Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, CyprusDepartment of Physiology, Faculty of Medicine, Near East University, Nicosia, CyprusDepartment of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, CyprusGen Era Diagnostics Inc. Barbaros, Istanbul, TurkeyDepartment of Biostatistics, Faculty of Medicine, Near East University, Nicosia, CyprusDepartment of Internal Medicine, Faculty of Medicine, Pamukkale University, Denizli, TurkeyDepartment of Biochemistry, Faculty of Medicine, Pamukkale University, Denizli, TurkeyDepartment of Surgery, Faculty of Medicine, Pamukkale University, Denizli, Turkey Introduction: Despite the numerous studies demonstrating gut microbiota dysbiosis in obese subjects, there is no data on the association between obesity and gastric microbiota. The aim of this study was to address this gap in literature by comparing the composition of gastric microbiota in obese patients and a control group which included normal weight volunteers diagnosed with functional dyspepsia (FD). Methodology: A total of 19 obese patients, and 18 normal weight subjects with FD and normal endoscopy results were included in the study. The gastric tissue samples were collected from participants in both groups by bariatric surgery and endoscopy, respectively, and profiled using 16S ribosomal RNA gene sequencing. Results: There was no significant difference in the α-diversity scores, while distinct gastric microbial compositions were detected in both groups. Significantly lower levels of Bacteroidetes and Fusobacteria, and higher Firmicutes/Bacteroidetes ratio were recorded in the obese patients. A total of 15 bacterial genera exhibited significant difference in gastric abundance with Prevotella_7, Veillonella, Cupriavidus, and Acinetobacter, present in frequencies higher than 3% in at least one subject group. Conclusions: Our study suggests a significant association between obesity and gastric microbiome composition. Future studies with larger sample size and gastric samples from subjects without any gastrointestinal complications are required to confirm our conclusions. https://jidc.org/index.php/journal/article/view/19304obesenormalgastricmicrobiota16S rRNA |
| spellingShingle | Umut Gazi Gunnur Kocer Emrah Ruh Can Holyavkin Ozgur Tosun Mustafa Celik Aysegul Cort Donmez Onur Birsen Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia Journal of Infection in Developing Countries obese normal gastric microbiota 16S rRNA |
| title | Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| title_full | Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| title_fullStr | Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| title_full_unstemmed | Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| title_short | Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| title_sort | gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia |
| topic | obese normal gastric microbiota 16S rRNA |
| url | https://jidc.org/index.php/journal/article/view/19304 |
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