Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)

Abstract The diversity of fish coloration is a fascinating scientific question. The pawak croaker (Pennahia pawak) is distinguished by its silver-white colour, setting it apart from other Sciaenidae species. However, the genomic information of this genus remains largely unexplored. This study aims t...

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Main Authors: Lihua Jiang, Peng Zheng, Jialang Zheng, Yifan Liu, Weihua Song, Shun Chen, Wangyang Jin, Xiaojun Yan
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04745-y
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author Lihua Jiang
Peng Zheng
Jialang Zheng
Yifan Liu
Weihua Song
Shun Chen
Wangyang Jin
Xiaojun Yan
author_facet Lihua Jiang
Peng Zheng
Jialang Zheng
Yifan Liu
Weihua Song
Shun Chen
Wangyang Jin
Xiaojun Yan
author_sort Lihua Jiang
collection DOAJ
description Abstract The diversity of fish coloration is a fascinating scientific question. The pawak croaker (Pennahia pawak) is distinguished by its silver-white colour, setting it apart from other Sciaenidae species. However, the genomic information of this genus remains largely unexplored. This study aims to advance our understanding by assembling and annotating the genome of P. pawak at the chromosome level using multi-platform sequencing data. The assembled genome size of P. pawak is 613.02 Mb, closely matching the estimated size of 570.32 Mb from 21-mer analysis. The assembly features a scaffold N50 of 27.09 Mb, with 24 chromosomes successfully constructed using Hi-C technology, achieving a mounting rate of 99.31%. Genome annotation revealed that 26.20% of the genome consists of repetitive sequences and identified 26,361 protein-coding genes, of which 25,885 have functional annotations. This chromosome-level genome assembly of P. pawak provides valuable resources for comparative genomic studies within the Sciaenidae family and offers foundational data for researchers to understand its unique silver-white pigmentation.
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spelling doaj-art-19570a02fac44edebb3cb3b6a931be342025-08-20T02:56:14ZengNature PortfolioScientific Data2052-44632025-03-0112111010.1038/s41597-025-04745-yChromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)Lihua Jiang0Peng Zheng1Jialang Zheng2Yifan Liu3Weihua Song4Shun Chen5Wangyang Jin6Xiaojun Yan7National Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityMarine Science and Technology College, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityNational Engineering Research Center for Marine Facilities Aquaculture, Zhejiang Ocean UniversityAbstract The diversity of fish coloration is a fascinating scientific question. The pawak croaker (Pennahia pawak) is distinguished by its silver-white colour, setting it apart from other Sciaenidae species. However, the genomic information of this genus remains largely unexplored. This study aims to advance our understanding by assembling and annotating the genome of P. pawak at the chromosome level using multi-platform sequencing data. The assembled genome size of P. pawak is 613.02 Mb, closely matching the estimated size of 570.32 Mb from 21-mer analysis. The assembly features a scaffold N50 of 27.09 Mb, with 24 chromosomes successfully constructed using Hi-C technology, achieving a mounting rate of 99.31%. Genome annotation revealed that 26.20% of the genome consists of repetitive sequences and identified 26,361 protein-coding genes, of which 25,885 have functional annotations. This chromosome-level genome assembly of P. pawak provides valuable resources for comparative genomic studies within the Sciaenidae family and offers foundational data for researchers to understand its unique silver-white pigmentation.https://doi.org/10.1038/s41597-025-04745-y
spellingShingle Lihua Jiang
Peng Zheng
Jialang Zheng
Yifan Liu
Weihua Song
Shun Chen
Wangyang Jin
Xiaojun Yan
Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
Scientific Data
title Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
title_full Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
title_fullStr Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
title_full_unstemmed Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
title_short Chromosome-level genome assembly and annotation of pawak croaker (Pennahia pawak)
title_sort chromosome level genome assembly and annotation of pawak croaker pennahia pawak
url https://doi.org/10.1038/s41597-025-04745-y
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